Clone Read LE_HBa0120H21_T7_87545

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0120H21_T7_87545
Primer
T7
ID from Seq. Facility
LE_HBa_120_H21-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
676 trimmed for vector and quality, 816 raw
Sequence
>LE_HBa0120H21_T7_87545 (raw untrimmed)
CTCGGTAACCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTGTGGATCCCTTAGCATTGGTCTTGCCCAATCCACCAAGATTCGCTCTTT
GAGGGGGCGTTTCTTGTCCATGGCTCGTCGTCCTGTTATAAGTTCTAACAAAACTACTCCGAAGCTGTACACATCGCTCTTGCTTGTCAAATGACCTGTT
TAAAGAAAATGAAAGTATAAGTATTCGTAATAAGTGGGACTACTAAGAAGTAACGCGATAAATGAGACACATAACATCTTAAAGTACACTGATAATCTAA
AAAATTACCAGTCATGATGTACTCCGGAGCAGCATAACCATGAGTGCCCATCACACGAGTTGAGACATGTGTGTCATCGCCTTCCGGTCCATCCTTTGCT
AGCCCAAAATCAGATAGTTTGGCTCTGTAATCCTGATAATTCATAGCATTATGAGTTTCTAGCTGAAAAAGGTGTTGAACTTTTCAATGTCAAAACAATA
CTTCGTAGATAACTTACCGAGTCTAAGAGGATGTTAGAAGCCTTAAAATCGCGGTAGATTACTGGTTTTTCTTCTCCGTGAAGGAAAGCCAGTCCCTTTG
CAGCACCAACCATAATTTTTATTCTGGTCAACCATGGCAAACAACTCGAATATCCTGTTTTGATACATCACATGAAAAATCAGAAACCGAGAACGGGGAC
AAAAGTTTTTTGTTCATTTTACAATCGATTCATACAAAAAAATTCATGCCTTACCCGAAAAAAGTTGATTCCTTCGAGGTTTTCCCTTTGCCATTATCCT
CGTTAAACCAAAAAAT
>LE_HBa0120H21_T7_87545 (trimmed for quality and cloning vector)
AGCTTGTGTGGATCCCTTAGCATTGGTCTTGCCCAATCCACCAAGATTCGCTCTTTGAGGGGGCGTTTCTTGTCCATGGCTCGTCGTCCTGTTATAAGTT
CTAACAAAACTACTCCGAAGCTGTACACATCGCTCTTGCTTGTCAAATGACCTGTTTAAAGAAAATGAAAGTATAAGTATTCGTAATAAGTGGGACTACT
AAGAAGTAACGCGATAAATGAGACACATAACATCTTAAAGTACACTGATAATCTAAAAAATTACCAGTCATGATGTACTCCGGAGCAGCATAACCATGAG
TGCCCATCACACGAGTTGAGACATGTGTGTCATCGCCTTCCGGTCCATCCTTTGCTAGCCCAAAATCAGATAGTTTGGCTCTGTAATCCTGATAATTCAT
AGCATTATGAGTTTCTAGCTGAAAAAGGTGTTGAACTTTTCAATGTCAAAACAATACTTCGTAGATAACTTACCGAGTCTAAGAGGATGTTAGAAGCCTT
AAAATCGCGGTAGATTACTGGTTTTTCTTCTCCGTGAAGGAAAGCCAGTCCCTTTGCAGCACCAACCATAATTTTTATTCTGGTCAACCATGGCAAACAA
CTCGAATATCCTGTTTTGATACATCACATGAAAAATCAGAAACCGAGAACGGGGACAAAAGTTTTTTGTTCATTTT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.974267
Expected error %
0.429762
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g05940.1 Score 87 E Value 7e-52 Identity 73.70% Align. Length 605bp (89.50%) Frame 1
68415.m00645 protein kinase, putative similar to auxin-regulated dual specificity cytosolic kinase [Lycopersicon esculentum] gi|14484938|gb|AAK62821
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g35580.1 Score 86 E Value 5e-47 Identity 68.40% Align. Length 593bp (87.72%) Frame 1
68418.m04236 protein kinase, putative similar to auxin-regulated dual specificity cytosolic kinase [Lycopersicon esculentum] gi|14484938|gb|AAK62821
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAK62821.1| Score 107 E Value 1e-65 Identity 97.00% Align. Length 605bp (89.50%) Frame 1
auxin-regulated dual specificity cytosolic kinase [Lycopersicon esculentum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_479146.1| Score 92 E Value 5e-51 Identity 77.40% Align. Length 587bp (86.83%) Frame 1
putative auxin-regulated dual specificity cytosolic kinase [Oryza sativa (japonica cultivar-group)] dbj|BAC80085.1| putative auxin-regulated dual specificity cytosolic kinase [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0120H21
Clone
Clone name
hba-120h21
LE_HBa0120H21
C06HBa0120H21
P120H21
Clone type
BAC
Contamination
none
Estimated length
124,794
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found