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Clone Read LE_HBa0075L24_SP6_85438

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0075L24_SP6_85438
Primer
SP6
ID from Seq. Facility
LE_HBa_075_L24-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
688 trimmed for vector and quality, 847 raw
Sequence
>LE_HBa0075L24_SP6_85438 (raw untrimmed)
GGCCCGAGGTTTCAGCAAACACGCCATCGATAATAAGTTTAAGAAAATTTCATACACAATTAAAGAATGTTAAAGCACAGCATAGTCGGTTCCAAACCTC
GAAATGATTAAGGTTGCAGTAAATTGAAAACTAATCGAACTATGACGCATTCATTGCAGTAAAATACTGATATAACGGATTGCATATAATCTGCCCCAAC
TAATTTAGGGTTCAGGTTTGGTTGATTGATTGATAGTGGCATAGCAACAATAAAACCTCAACAAACGAATCTGAAACACTGATGTACCTTTATTGTGATA
GTGCCCCGATCCTTCTTATCTTTGACTTTGAGCATATCAAGTTTTGGAAGTAATCTTAATTCAATTTCTTTAGCAGCATCAGCTACAAGCTCATTCAGAG
GCAACTTTGCGACACCCATTCGCTGGTCTTGCCCAATATTGTCTTTATCAAAGACCTGCTCAAAATGACGTGAAGCTCAACTATCACACATATCCACTTA
AAACAGCAGGAAGTACAGCACCCGTAGATATTCAAGACAGAGCAGAGCACAGGAATGTTGAAATCAAACCTCAATGAATAGGGATTGCGTCTCCTTGTCT
TCTGCAATTAATTCAAACGTCTGATCCCAAACAGGATTTAGGTTGTTGTCAATGGTTTTTGTCTTAACCTTGAAAAGGGGGCGTATGTGTACAACAGCAT
ATGGATCGGATTTTCCTATCATTTCGTGGTTCTTCAAGCCATTAGCCTTGACAATAGTTTACTGTGAGCTTCCCTTGTGGTTAAGTTCCAAATTACTGCA
GGGAGGGAAATACTGGTACCATTGATGAAAATAAGCCGGAGAGAAGA
>LE_HBa0075L24_SP6_85438 (trimmed for quality and cloning vector)
GCCATCGATAATAAGTTTAAGAAAATTTCATACACAATTAAAGAATGTTAAAGCACAGCATAGTCGGTTCCAAACCTCGAAATGATTAAGGTTGCAGTAA
ATTGAAAACTAATCGAACTATGACGCATTCATTGCAGTAAAATACTGATATAACGGATTGCATATAATCTGCCCCAACTAATTTAGGGTTCAGGTTTGGT
TGATTGATTGATAGTGGCATAGCAACAATAAAACCTCAACAAACGAATCTGAAACACTGATGTACCTTTATTGTGATAGTGCCCCGATCCTTCTTATCTT
TGACTTTGAGCATATCAAGTTTTGGAAGTAATCTTAATTCAATTTCTTTAGCAGCATCAGCTACAAGCTCATTCAGAGGCAACTTTGCGACACCCATTCG
CTGGTCTTGCCCAATATTGTCTTTATCAAAGACCTGCTCAAAATGACGTGAAGCTCAACTATCACACATATCCACTTAAAACAGCAGGAAGTACAGCACC
CGTAGATATTCAAGACAGAGCAGAGCACAGGAATGTTGAAATCAAACCTCAATGAATAGGGATTGCGTCTCCTTGTCTTCTGCAATTAATTCAAACGTCT
GATCCCAAACAGGATTTAGGTTGTTGTCAATGGTTTTTGTCTTAACCTTGAAAAGGGGGCGTATGTGTACAACAGCATATGGATCGGA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.972136
Expected error %
0.163626
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g61050.1 Score 114 E Value 4e-26 Identity 15.40% Align. Length 428bp (62.21%) Frame 3
68416.m06832 calcium-dependent lipid-binding protein, putative strong similarity to CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam profile PF00168: C2 domain
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g61030.1 Score 74 E Value 7e-14 Identity 14.60% Align. Length 308bp (44.77%) Frame 3
68416.m06828 C2 domain-containing protein similar to CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam profile PF00168: C2 domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T04143 Score 153 E Value 3e-36 Identity 21.10% Align. Length 425bp (61.77%) Frame 3
CLB1 protein - tomato dbj|BAA24382.1| CLB1 [Lycopersicon esculentum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB94141.1| Score 114 E Value 2e-24 Identity 15.40% Align. Length 428bp (62.21%) Frame 3
CaLB protein [Arabidopsis thaliana] gb|AAO42810.1| At3g61050 [Arabidopsis thaliana] ref|NP_191664.1| calcium-dependent lipid-binding protein, putative [Arabidopsis thaliana] pir||T50526 CaLB protein - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0075L24
Clone
Clone name
hba-75l24
LE_HBa0075L24
C01HBa0075L24
P075L24
Clone type
BAC
Contamination
none
Estimated length
89,282
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found