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Clone Read LE_HBa0166L22_SP6_78522

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0166L22_SP6_78522
Primer
SP6
ID from Seq. Facility
LE_HBa_166_L22-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
660 trimmed for vector and quality, 778 raw
Sequence
>LE_HBa0166L22_SP6_78522 (raw untrimmed)
CATTGGAGGCTAGTGGACTAAAGGGGGAATTTCAATGCTATGGGATAGTAGGGCCTGGGAAGGCGACATTCTGGAAACTGGTATATACTTTTTGATCTGT
TTTGAGTCTCAATTTTATAACTGCTCTTGCCATATCACGGGGGTGTATGCCCCTAACTATTTTATAGAAAAAAGATCTGTCTGGGAGGAAATGGGGCTAC
AAGGGGAATGAAGAAGTTTTTGAGAAAAACTTTCATGCAACATTCATTGCACTCATACTAAAGAAGAAATGAGCAAATGAACTAAGGCACTTCAGACCTA
TAAGCCTGATAGGTAGCATCTATAAACTACTCTCCAAAGTACTGACTGAGAGGCTGATGTTAATGATTGGAAAATTGGTTGATTCCCAACAGACCGCATT
TATTAAAGGTAGACAGATCATGGATGTTGTGTTGATTGCTTATGGAGTTGTAGATTCCAGAATCAAACAGAAGAAGCCTGATATACTATGCAAGTTGGAC
TTAGAGAAAGCATATGATTATGTCAACTGGAGGTATTTATTGAAGATTCTGGATAGAATGGGCTTCGAAAAAAAAGACTGAGACGGGTAAAGTATTATAT
TTCAACTGTCAAATTTTCCGTGCTTACTAATGGAGCCTCTATAGGATTTTTTCAATACTCAGAGAGGTCTCCGGCAAGGTGACCCTTTGTCACCTTTTCT
GTTTTTAATTCTTATTGGAGGGGCTGAAATATATGAATAAAACAACCAAATAATAAAAGCTGGGTTTAAAATGGTTTT
>LE_HBa0166L22_SP6_78522 (trimmed for quality and cloning vector)
TGGACTAAAGGGGGAATTTCAATGCTATGGGATAGTAGGGCCTGGGAAGGCGACATTCTGGAAACTGGTATATACTTTTTGATCTGTTTTGAGTCTCAAT
TTTATAACTGCTCTTGCCATATCACGGGGGTGTATGCCCCTAACTATTTTATAGAAAAAAGATCTGTCTGGGAGGAAATGGGGCTACAAGGGGAATGAAG
AAGTTTTTGAGAAAAACTTTCATGCAACATTCATTGCACTCATACTAAAGAAGAAATGAGCAAATGAACTAAGGCACTTCAGACCTATAAGCCTGATAGG
TAGCATCTATAAACTACTCTCCAAAGTACTGACTGAGAGGCTGATGTTAATGATTGGAAAATTGGTTGATTCCCAACAGACCGCATTTATTAAAGGTAGA
CAGATCATGGATGTTGTGTTGATTGCTTATGGAGTTGTAGATTCCAGAATCAAACAGAAGAAGCCTGATATACTATGCAAGTTGGACTTAGAGAAAGCAT
ATGATTATGTCAACTGGAGGTATTTATTGAAGATTCTGGATAGAATGGGCTTCGAAAAAAAAGACTGAGACGGGTAAAGTATTATATTTCAACTGTCAAA
TTTTCCGTGCTTACTAATGGAGCCTCTATAGGATTTTTTCAATACTCAGAGAGGTCTCCG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.952714
Expected error %
0.232171
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL78659.1| Score 117 E Value 6e-28 Identity 61.10% Align. Length 370bp (56.06%) Frame 3
reverse transcriptase [Fagus sylvatica]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA73819.1| Score 100 E Value 5e-20 Identity 16.30% Align. Length 275bp (41.67%) Frame 3
reverse transcriptase [Antirrhinum majus] pir||T17102 RNA-directed DNA polymerase (EC 2.7.7.49) (clone AmLi2) - garden snapdragon (fragment)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0166L22
Clone
Clone name
hba-166l22
LE_HBa0166L22
P166L22
Clone type
BAC
Contamination
none
Estimated length
91,286
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found