Clone Read LE_HBa0157G16_SP6_75966

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0157G16_SP6_75966
Primer
SP6
ID from Seq. Facility
LE_HBa_157_G16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
521 trimmed for vector and quality, 858 raw
Genbank Accession
DU063130
Sequence
>LE_HBa0157G16_SP6_75966 (raw untrimmed)
GTGGCTGGACCTCATCACTGTTATGTAACCACTTCCATCCTTGTCAAAATATTGAAATGCTGCCATGAGACCTTTAACGCGCTCCAATTTATTAAGATGA
ACAGTTGCCGCACTGAATTCTCCATAGTCAATAGTTCCACTATTGTCCACATCAGCCTGAAATTGTGAGTTAGCTACATCAATCACCTTAGATAATTTAA
AGGAGCTTTGCATGAACCTCATAGAAACACATGTTTGTAGTTGAGGGACACTAGAAAGATCACCAGAGAAATCATGTGGATTGAAATAAGAGATAAATGA
TTCCAAGTCAGAGTTTAGCTAGAACAATTTACCTCAAACTTTCAAGGACTTGTGACACACAAAAAAAAAGCTGAGTAACTTGAAAAATGAACTTCAGAAG
CTACAGAAGTTCTTGATAATTTAACGCAGGAGAGTGTTTATCCCCGCCTCAACTCTCCACTTTCCAAACAGATAGAATTCACCATCATTAACTTGTGCAA
TTCTACAGAGATGAAGATTCTGAACTTCCATAGTAGACAGTCTCGTCATTTTTTTTTTTTTTTTTTATGTTTTTTTTTTTTCTTTTAATGACTTTGCAAT
AAAGATTTCCTCACCGCATCCATAATTTTTTTTTTTTTTTTTCCCTTAGAANGNAGAGGCGTATCGTCTTAACCCATGCATTTAGATCATCAAGTGTAAT
TGCAGCAATATTGGTGGGATTAGATGGATATGAAGATTTGTAGCGGAGTGTATGTTATTTGTGCAAATAACCTTTTTGTCAATTACCTTGTAAGAACCCA
CGAGGGAAACCTAATAAATTTTGATAAAAGATAATAAAAGATAAGGGTTATGGAAACA
>LE_HBa0157G16_SP6_75966 (trimmed for quality and cloning vector)
TAACCACTTCCATCCTTGTCAAAATATTGAAATGCTGCCATGAGACCTTTAACGCGCTCCAATTTATTAAGATGAACAGTTGCCGCACTGAATTCTCCAT
AGTCAATAGTTCCACTATTGTCCACATCAGCCTGAAATTGTGAGTTAGCTACATCAATCACCTTAGATAATTTAAAGGAGCTTTGCATGAACCTCATAGA
AACACATGTTTGTAGTTGAGGGACACTAGAAAGATCACCAGAGAAATCATGTGGATTGAAATAAGAGATAAATGATTCCAAGTCAGAGTTTAGCTAGAAC
AATTTACCTCAAACTTTCAAGGACTTGTGACACACAAAAAAAAAGCTGAGTAACTTGAAAAATGAACTTCAGAAGCTACAGAAGTTCTTGATAATTTAAC
GCAGGAGAGTGTTTATCCCCGCCTCAACTCTCCACTTTCCAAACAGATAGAATTCACCATCATTAACTTGTGCAATTCTACAGAGATGAAGATTCTGAAC
TTCCATAGTAGACAGTCTCGT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.954242
Expected error %
0.93572
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g35310.1 Score 78 E Value 3e-15 Identity 20.50% Align. Length 194bp (37.24%) Frame 1
68417.m05019 calcium-dependent protein kinase, putative / CDPK, putative similar to calmodulin-domain protein kinase CDPK isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g17290.1 Score 76 E Value 9e-15 Identity 20.00% Align. Length 194bp (37.24%) Frame 1
68415.m01997 calcium-dependent protein kinase isoform 6 (CPK6) identical to calmodulin-domain protein kinase CDPK isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAW31900.1| Score 82 E Value 9e-15 Identity 21.50% Align. Length 194bp (37.24%) Frame 1
calcium-dependent/calmodulin-independent protein kinase [Panax ginseng]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP55748.1| Score 81 E Value 1e-14 Identity 24.10% Align. Length 161bp (30.90%) Frame 1
calcium-dependent protein kinase 3 [Capsicum annuum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0157G16
Clone
Clone name
hba-157g16
LE_HBa0157G16
P157G16
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found