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Clone Read LE_HBa0154K21_T7_75401

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0154K21_T7_75401
Primer
T7
ID from Seq. Facility
LE_HBa_154_K21-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
628 trimmed for vector and quality, 914 raw
Genbank Accession
DU066154
Sequence
>LE_HBa0154K21_T7_75401 (raw untrimmed)
CCTAACCGGGTCCGGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGCATTATAGTCCTGTCACACCCCTTGAAACCAACATTTTATTAGTA
AAACTAGTATTGTCAATCTCATAAAACGTATATTCTATTTTAAGAAAAAAAAAAAAAGAAGAATTACCGAGTCCAGTAAAATATTGGCCGTTTTAAAATC
GCGGTAAATAACTTGTTTTTCTATGGTGTGTAAGAAATCAAGTCCCTTCGCTGCTCCGATTGCTATTTTCAGCCTTGTACCCCATGACAATGCTTCAGCG
CCTTCTGCTAATAAAATCGAATATTTTCTTTTTATAATGTCAATGTACATAAATTAAATCGAGTTAATGAAAGAATAGTATTTGTAAGGTGCCTTACTTC
TGAAAAGATGACTTTCTAAGCTCCCTTTTTGCATGTGCTCATAGACTAGAAGAAATGTTTTGTCTTCCCAACAATATCCAATTAGTTTTACTAAATTTGG
GTGGCTAAGTTTTCCCAAAAACTTTACTTCAGCCTGCAAAAACAAATTTTACATAATCAATTTTCGATAGAAAACACTAATATATTTTTATTATTGATAG
AAATTTTTTTTTGAACTGTTGCATCATTTATATTTTTATTATTTTACTATTTCAAATTAGAAAGTCGAGTCATACATAATGAATGGAATGCAGTTTCAAT
TAGAGGAAAAGACTTAAGGATTTTAGTTAGAGGGGCTGACTTCCGAACCCCCTAATGCCTTCATCAGAATGTTCTTGCATTTTTTATCTTTTTCTTACAT
TAGATCATCGAATCATGTTTTCATTGTCCATTGCAATTTATGTAGAAAAATGGAGTTCTTAAAGCGACCTGAACGCGGTTTTTTTTTTTATCTTTTATGA
TCTTTTATTTTTTC
>LE_HBa0154K21_T7_75401 (trimmed for quality and cloning vector)
AGCTTTGCATTATAGTCCTGTCACACCCCTTGAAACCAACATTTTATTAGTAAAACTAGTATTGTCAATCTCATAAAACGTATATTCTATTTTAAGAAAA
AAAAAAAAAGAAGAATTACCGAGTCCAGTAAAATATTGGCCGTTTTAAAATCGCGGTAAATAACTTGTTTTTCTATGGTGTGTAAGAAATCAAGTCCCTT
CGCTGCTCCGATTGCTATTTTCAGCCTTGTACCCCATGACAATGCTTCAGCGCCTTCTGCTAATAAAATCGAATATTTTCTTTTTATAATGTCAATGTAC
ATAAATTAAATCGAGTTAATGAAAGAATAGTATTTGTAAGGTGCCTTACTTCTGAAAAGATGACTTTCTAAGCTCCCTTTTTGCATGTGCTCATAGACTA
GAAGAAATGTTTTGTCTTCCCAACAATATCCAATTAGTTTTACTAAATTTGGGTGGCTAAGTTTTCCCAAAAACTTTACTTCAGCCTGCAAAAACAAATT
TTACATAATCAATTTTCGATAGAAAACACTAATATATTTTTATTATTGATAGAAATTTTTTTTTGAACTGTTGCATCATTTATATTTTTATTATTTTACT
ATTTCAAATTAGAAAGTCGAGTCATACA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.932663
Expected error %
0.814239
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g76360.1 Score 128 E Value 2e-30 Identity 18.40% Align. Length 368bp (58.60%) Frame 1
68414.m08872 protein kinase, putative similar to protein kinase APK1B, SWISS-PROT:P46573; contains protein kinase domain, Pfam:PF00069
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g17220.2 Score 123 E Value 9e-29 Identity 18.30% Align. Length 365bp (58.12%) Frame 1
68415.m01989 protein kinase, putative similar to protein kinase APK1A [Arabidopsis thaliana] Swiss-Prot:Q06548
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_177762.3| Score 128 E Value 1e-28 Identity 18.40% Align. Length 368bp (58.60%) Frame 1
protein kinase, putative [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF16665.1| Score 128 E Value 1e-28 Identity 18.40% Align. Length 368bp (58.60%) Frame 1
putative protein kinase; 59396-62219 [Arabidopsis thaliana] pir||B96791 hypothetical protein F15M4.14 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0154K21
Clone
Clone name
hba-154k21
LE_HBa0154K21
P154K21
Clone type
BAC
Contamination
none
Estimated length
145,318
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found