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Clone Read LE_HBa0033P12_T7_7271

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0033P12_T7_7271
Primer
T7
ID from Seq. Facility
LE_HBa_033_P12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
835 trimmed for vector and quality, 872 raw
Genbank Accession
DU026630
Sequence
>LE_HBa0033P12_T7_7271 (raw untrimmed)
CGTTGAATCATTAAGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCCAGAGATATCTCATCAACTGGTTCACTGTCTTTCGCAAAATGCAA
CACCCTTGTGCACCGTGTCAATATAAAAAGCATGCGTGTCAACAACCACTTCAAAGGACCGATTTGGCATTCTTGTCTTCTTTTTGTCAGGTCCTGGACA
ATTGGTTCACACTTGGAGCGAAACTCTAAGCAGGTCATCTTGATACACTCTTGAGCAAGAACAACAAGTTCTTCAGCCATCTTTTGTCCATCCAGAGGCA
AGGATTCATTCTTCTGTACAATTTCTACAACCTCCTGCAAGAAACCACCTAGCTCTGTGTTCACCGCTTCTTTTGCAGCATCAAATCTCAAACGAAGAGA
TCCAAGAAGTTCCTGCATGTAATGCCATGGAATCAAAGTTAATAATGAACATCAAAAACCATATCAGCCACCCAACTGACCTACTTATCTTGATCATTAT
ACACTGAAACGAATGTAATTCCTTCATATGCTTATCTCTTAACACCCTTACATAATATCTGGAAGTACCAGAACCTAATTCCTTCCTATGCTTATCACTT
ACCACCCCTCACATAATAACTGGAAATACCAGAACCAGAAATGTAAAAAAAATTACCTTAAGATCATTATAGCTGTGAGCTCGTGGAGGTGATGGTTGAA
TGCCACCTTTTGGACCTAGCTCATGTGAAAAACTTTTGAATGTTTTGCTTGCTTTACTAATAGGCATTGCTCCTTGGGAAGATTGCCTCACTGTCACATG
CTTTCCCATCATATGCACATTCCTTCCATGACCCTCCTTGTCAGCCCCCTAAAATTGCAAACTATCATCCAC
>LE_HBa0033P12_T7_7271 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCCAGAGATATCTCATCAACTGGTTCACTGTCTTTCGCAAAATGCAACACCCTTGTGCACCGTGTCAAT
ATAAAAAGCATGCGTGTCAACAACCACTTCAAAGGACCGATTTGGCATTCTTGTCTTCTTTTTGTCAGGTCCTGGACAATTGGTTCACACTTGGAGCGAA
ACTCTAAGCAGGTCATCTTGATACACTCTTGAGCAAGAACAACAAGTTCTTCAGCCATCTTTTGTCCATCCAGAGGCAAGGATTCATTCTTCTGTACAAT
TTCTACAACCTCCTGCAAGAAACCACCTAGCTCTGTGTTCACCGCTTCTTTTGCAGCATCAAATCTCAAACGAAGAGATCCAAGAAGTTCCTGCATGTAA
TGCCATGGAATCAAAGTTAATAATGAACATCAAAAACCATATCAGCCACCCAACTGACCTACTTATCTTGATCATTATACACTGAAACGAATGTAATTCC
TTCATATGCTTATCTCTTAACACCCTTACATAATATCTGGAAGTACCAGAACCTAATTCCTTCCTATGCTTATCACTTACCACCCCTCACATAATAACTG
GAAATACCAGAACCAGAAATGTAAAAAAAATTACCTTAAGATCATTATAGCTGTGAGCTCGTGGAGGTGATGGTTGAATGCCACCTTTTGGACCTAGCTC
ATGTGAAAAACTTTTGAATGTTTTGCTTGCTTTACTAATAGGCATTGCTCCTTGGGAAGATTGCCTCACTGTCACATGCTTTCCCATCATATGCACATTC
CTTCCATGACCCTCCTTGTCAGCCCCCTAAAATTG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.967095
Expected error %
0.0395773
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|S52393.1:14-153 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (3.83%) Frame 2
{polylinker} [pIC20HE, Plasmid Recombinant, 153 nt]
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U46493.1:1-95 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (3.83%) Frame 2
Cloning vector pFlp recombinase gene, complete cds
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g45160.1 Score 126 E Value 1e-29 Identity 15.90% Align. Length 383bp (45.87%) Frame 2
68414.m05177 protein kinase family protein contains eukaryotic protein kinase domain, INTERPRO:IPR000719
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g17850.1 Score 99 E Value 3e-21 Identity 15.30% Align. Length 338bp (40.48%) Frame 2
68416.m02275 protein kinase, putative similar to IRE (incomplete root hair elongation) [Arabidopsis thaliana] gi|6729346|dbj|BAA89783; contains protein kinase domain Pfam:PF00069
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_175130.1| Score 126 E Value 6e-28 Identity 15.90% Align. Length 383bp (45.87%) Frame 2
protein kinase family protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||H96509 Score 126 E Value 6e-28 Identity 15.90% Align. Length 383bp (45.87%) Frame 2
protein F27F5.23 [imported] - Arabidopsis thaliana gb|AAF69167.1| F27F5.23 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0033P12
Clone
Clone name
hba-33p12
LE_HBa0033P12
P033P12
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found