Clone Read LE_HBa0081A20_T7_67239

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0081A20_T7_67239
Primer
T7
ID from Seq. Facility
LE_HBa_081_A20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
726 trimmed for vector and quality, 801 raw
Sequence
>LE_HBa0081A20_T7_67239 (raw untrimmed)
GCCTCGGTCCGCGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAACACCACTACTATATAACTGCACTCAAAACTCTCCAGCAACTGAAATCAT
TAAGATTCTACTTGTTCATCCTCCTCATCTTCATAGTCATAACCTTCCTCTTCAGCTGTTGCATCTTGATACTGCTGGTACTCGGAAACTAAATCGTTCA
TGTTGCTTTCAGCCTCGGTAAACTCCATCTCATCCATACCTTCTCCAGTGTACCAATGCAAGAAAGCCTTTCTTCGGAACATAGCTGTAAACTGCTCACT
GACCCTACGGAACATCTCCTGAATCGAAGTGGAGTTACCAATGAAAGTTGAGGCCATCTTCAGACCAGTAGGAGGGATGTCACAGACTGTTGACTTAACA
TTGTTAGGGATCCACTCAACAAAATAGGAAGAGTTCTTGTTCTGCACATTAATCATCTGTTCATCGACTTCCTTGGTGCTCATCTTTCCACGGAACATAG
CTGAAGCAGTCAAATATCGACCATGACGAGGATCAGCAGCACACATCATATTCTTTGCATCCCACATCTGCTGTGTTAGCTCAGGAACAGTCAATGCTCG
ATATTGCTGCGACCCACGAGAAGTGAGCGGAGCAAATCCAACCATGAAGAAATGCAACCGAGGGAATGGGATGAGGTTAACAGCAAGCTTGCGGAGATCA
GAGTTGAGTTGACCAGGGAAACGGAGACAGCAAGTTACACCACTCATGGTGGCAGAAATCAAATGGTTTAGGTCCCCAACTGAGGCAAACATCAATTCAT
A
>LE_HBa0081A20_T7_67239 (trimmed for quality and cloning vector)
AGCTTAACACCACTACTATATAACTGCACTCAAAACTCTCCAGCAACTGAAATCATTAAGATTCTACTTGTTCATCCTCCTCATCTTCATAGTCATAACC
TTCCTCTTCAGCTGTTGCATCTTGATACTGCTGGTACTCGGAAACTAAATCGTTCATGTTGCTTTCAGCCTCGGTAAACTCCATCTCATCCATACCTTCT
CCAGTGTACCAATGCAAGAAAGCCTTTCTTCGGAACATAGCTGTAAACTGCTCACTGACCCTACGGAACATCTCCTGAATCGAAGTGGAGTTACCAATGA
AAGTTGAGGCCATCTTCAGACCAGTAGGAGGGATGTCACAGACTGTTGACTTAACATTGTTAGGGATCCACTCAACAAAATAGGAAGAGTTCTTGTTCTG
CACATTAATCATCTGTTCATCGACTTCCTTGGTGCTCATCTTTCCACGGAACATAGCTGAAGCAGTCAAATATCGACCATGACGAGGATCAGCAGCACAC
ATCATATTCTTTGCATCCCACATCTGCTGTGTTAGCTCAGGAACAGTCAATGCTCGATATTGCTGCGACCCACGAGAAGTGAGCGGAGCAAATCCAACCA
TGAAGAAATGCAACCGAGGGAATGGGATGAGGTTAACAGCAAGCTTGCGGAGATCAGAGTTGAGTTGACCAGGGAAACGGAGACAGCAAGTTACACCACT
CATGGTGGCAGAAATCAAATGGTTTA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.979687
Expected error %
0.313271
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g23860.1 Score 421 E Value 1e-118 Identity 33.30% Align. Length 614bp (84.57%) Frame 3
68418.m02801 tubulin beta-8 chain (TUB8) (TUBB8) identical to SP|P29516 Tubulin beta-8 chain {Arabidopsis thaliana}; supporting cDNA gi|15451225|gb|AY054693.1|
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g29550.1 Score 417 E Value 1e-117 Identity 33.00% Align. Length 614bp (84.57%) Frame 3
68415.m03589 tubulin beta-7 chain (TUB7) identical to GB:M84704 SP|P29515 Tubulin beta-7 chain {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA49736.1| Score 421 E Value 1e-116 Identity 33.30% Align. Length 614bp (84.57%) Frame 3
Beta tubulin 1 [Lupinus albus] pir||S35142 tubulin beta chain - white lupine sp|P37392|TBB1_LUPAL Tubulin beta-1 chain (Beta-1 tubulin)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB03267.1| Score 421 E Value 1e-116 Identity 33.30% Align. Length 614bp (84.57%) Frame 3
beta-tubulin 2 sp|Q40106|TBB2_LUPAL Tubulin beta-2 chain (Beta-2 tubulin)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0081A20
Clone
Clone name
hba-81a20
LE_HBa0081A20
P081A20
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found