Notice: We have moved solgenomics to a new server and hosting system which caused some instability over the last few days. We apologize for any inconvenience.

Clone Read LE_HBa0033C18_T7_6659

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0033C18_T7_6659
Primer
T7
ID from Seq. Facility
LE_HBa_033_C18-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
860 trimmed for vector and quality, 924 raw
Genbank Accession
DU026081
Sequence
>LE_HBa0033C18_T7_6659 (raw untrimmed)
GCCTGGGTCCCGGGGGGAATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTAGATAAAGAAGAAATAACACCATATCATTATTTCATAACAGGAATA
ATAGACCAAAGAAAATATAAAATATTAATAAATACAGGACAAGAAGAAAATTATATAACAAGGGAGTTAGTATTAGAAGAAGAAATTATAAGAACAGGAT
ATACATGCCCTGGACTACCTAGTGAGATAGTGAACACGAATGAAGAAACAACAGAAAAAGAAATAATTATAGGAGGAATACCCTTAGTAATACAATTTAA
AATATACCAAGGAAATCATAATATTAAATTAGGAATAAAATGGTTAGAAAAAGTCAAACCATACAATATAGAAAATGAACAATTAACAATAACTTGTCAA
AATAAGAAAATAATTATAAAAAGGACAAAATGATTAATGAAAATATTTATACTTGCAAAAATTATAGTAGAAGGATATCACAACAGATATTATACCCCTA
TGATAGATACAGAAGCAGAAGCGAATATATGTAAATATAATTGCTTACCAGAAGATAAATGGGAAAAATTAAAAACACCTATGGTAGTAACGGGATTTAA
CAATGAAGGAAGTATGATTAAATACAAAGCAAAAAATATAAAAATACAAATATGGGATAAAATACTAACAATAGAAGAAATGTATAATTTTGAATTTACT
ACAAAAGATATGTTATTAGGAATGCCATTTCTAGATAGATATTATCCACACATAATAACAAAAACACATTGGTGGCTTACTACACCATGCGGAAATAAGA
TAGGAGCAAAAAGAGTAAATAATAAACAACAAAAACCTATGGAATGGATAAAAGGAAGTGAAAAGATAAACCAAGAAATGGAAATATAATAATAAAGCAA
TACGCATCAGAAATATTTATATTG
>LE_HBa0033C18_T7_6659 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTAGATAAAGAAGAAATAACACCATATCATTATTTCATAACAGGAATAATAGACCAAAGAAAATATAA
AATATTAATAAATACAGGACAAGAAGAAAATTATATAACAAGGGAGTTAGTATTAGAAGAAGAAATTATAAGAACAGGATATACATGCCCTGGACTACCT
AGTGAGATAGTGAACACGAATGAAGAAACAACAGAAAAAGAAATAATTATAGGAGGAATACCCTTAGTAATACAATTTAAAATATACCAAGGAAATCATA
ATATTAAATTAGGAATAAAATGGTTAGAAAAAGTCAAACCATACAATATAGAAAATGAACAATTAACAATAACTTGTCAAAATAAGAAAATAATTATAAA
AAGGACAAAATGATTAATGAAAATATTTATACTTGCAAAAATTATAGTAGAAGGATATCACAACAGATATTATACCCCTATGATAGATACAGAAGCAGAA
GCGAATATATGTAAATATAATTGCTTACCAGAAGATAAATGGGAAAAATTAAAAACACCTATGGTAGTAACGGGATTTAACAATGAAGGAAGTATGATTA
AATACAAAGCAAAAAATATAAAAATACAAATATGGGATAAAATACTAACAATAGAAGAAATGTATAATTTTGAATTTACTACAAAAGATATGTTATTAGG
AATGCCATTTCTAGATAGATATTATCCACACATAATAACAAAAACACATTGGTGGCTTACTACACCATGCGGAAATAAGATAGGAGCAAAAAGAGTAAAT
AATAAACAACAAAAACCTATGGAATGGATAAAAGGAAGTGAAAAGATAAACCAAGAAATG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.869442
Expected error %
0.133835
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 68 E Value 2e-12 Identity 100.00% Align. Length 33bp (3.84%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 68 E Value 2e-12 Identity 100.00% Align. Length 33bp (3.84%) Frame 2
pT7T318U cloning vector
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF08289.1| Score 280 E Value 4e-74 Identity 28.60% Align. Length 443bp (51.51%) Frame 1
polyprotein [tobacco vein-clearing virus] ref|NP_569141.1| polyprotein [tobacco vein-clearing virus]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB42622.1| Score 259 E Value 7e-68 Identity 26.30% Align. Length 440bp (51.16%) Frame 1
putative polyprotein (aspartic proteinase, reverse transcriptase, ribonuclease H) [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0033C18
Clone
Clone name
hba-33c18
LE_HBa0033C18
P033C18
Clone type
BAC
Contamination
none
Estimated length
133,063
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found