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Clone Read LE_HBa0042M21_SP6_63208

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0042M21_SP6_63208
Primer
SP6
ID from Seq. Facility
LE_HBa_042_M21-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
543 trimmed for vector and quality, 795 raw
Sequence
>LE_HBa0042M21_SP6_63208 (raw untrimmed)
ATTTTCTATCCTTCTTGAACTCTCAAAGTTCACCATTCCATCACCATCAACATCAACTTTCTTTATCATTTCCTTTAATGATCCAATTTCTTACCTTCTT
TCATTCCTAAAGACCCATAGTATCTTACTCAATTCTTCTTCTGATATTAATCCATCTTTATCATGATCAAACACATCAAAAGCTTCTTTAAGATCTCTCT
CTTTATTAGCAACTTCTTCTTCATTCATTTCACCTTCTTGTTCCACTCCATCTATTCCCAAGAAAGTATGACAAAGTTCACAAAACTCATCAAGATCAAT
TAAACCATCTTTGTTTGTATCAACTTTCTCAACCATATCAACAATATCTTTATCCTCCATTAATATCCCAATATTCTTTAGAGATAGCTTCAATTCTTGC
TTTGTTATGTAACCATCATTGTTCTTGTCAAAAGTTGCAAATATACCCCTTAGTTCAACTTTGTTGTAATTGTTAAAGCTTTTTGCCTTCTTTTGATCAA
CCATGGATGTTGTTGTTGATGATGATGATGGGGGTGTTGTTATGGTCCTTTCTTGGAGAGGTTGAGATTCTAGTGAGATTGATTGAATTAAAGAGTGGAA
CTTTGTTAGGTGGGAATTTGAAAATTGTTGTGATAAAGCCAATAATTGAATAGAATTGCTAAGAAAAGAATGTTTAATAGTGTCAACTATCTTTGCTTTG
CTTTGTTGATGGTTGAATTTTACTGGGAAATTGGAACACTGAAATTTAATATTAGTGGGTAAAATTATCGGATAATTTTTCTTATATCCAAATAA
>LE_HBa0042M21_SP6_63208 (trimmed for quality and cloning vector)
AGTTCACCATTCCATCACCATCAACATCAACTTTCTTTATCATTTCCTTTAATGATCCAATTTCTTACCTTCTTTCATTCCTAAAGACCCATAGTATCTT
ACTCAATTCTTCTTCTGATATTAATCCATCTTTATCATGATCAAACACATCAAAAGCTTCTTTAAGATCTCTCTCTTTATTAGCAACTTCTTCTTCATTC
ATTTCACCTTCTTGTTCCACTCCATCTATTCCCAAGAAAGTATGACAAAGTTCACAAAACTCATCAAGATCAATTAAACCATCTTTGTTTGTATCAACTT
TCTCAACCATATCAACAATATCTTTATCCTCCATTAATATCCCAATATTCTTTAGAGATAGCTTCAATTCTTGCTTTGTTATGTAACCATCATTGTTCTT
GTCAAAAGTTGCAAATATACCCCTTAGTTCAACTTTGTTGTAATTGTTAAAGCTTTTTGCCTTCTTTTGATCAACCATGGATGTTGTTGTTGATGATGAT
GATGGGGGTGTTGTTATGGTCCTTTCTTGGAGAGGTTGAGATT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.913458
Expected error %
0.23745
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g07490.1 Score 117 E Value 4e-27 Identity 14.80% Align. Length 437bp (80.48%) Frame 3
68416.m00893 calcium-binding protein, putative similar to calcium-binding protein GI:6580549 from [Lotus japonicus]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g05990.1 Score 112 E Value 1e-25 Identity 14.20% Align. Length 428bp (78.82%) Frame 3
68414.m00627 calcium-binding protein, putative strong similarity to calcium-binding protein [Lotus japonicus] GI:18413495; contains INTERPRO:IPR002048 calcium-binding EF-hand domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF02168.1| Score 117 E Value 2e-25 Identity 14.80% Align. Length 437bp (80.48%) Frame 3
putative calmodulin [Arabidopsis thaliana] ref|NP_187405.1| calcium-binding protein, putative [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAG48829.1| Score 112 E Value 5e-24 Identity 14.20% Align. Length 428bp (78.82%) Frame 3
putative calcium-binding protein [Arabidopsis thaliana] gb|AAF80122.1| Contains similarity to a calcium-binding protein from Lotus japonicus gi|6580549 and contains a EF hand PF|00036 domain. EST gb|T46471 comes from this gene. [Arabidopsis thaliana] ref|NP_172089.1| calcium-binding protein, putative [Arabidopsis thaliana] pir||H86194 hypothetical protein [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0042M21
Clone
Clone name
hba-42m21
LE_HBa0042M21
P042M21
Clone type
BAC
Contamination
none
Estimated length
89,953
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found