Clone Read LE_HBa0127L04_T7_60055

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0127L04_T7_60055
Primer
T7
ID from Seq. Facility
LE_HBa_127_L04-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
722 trimmed for vector and quality, 847 raw
Sequence
>LE_HBa0127L04_T7_60055 (raw untrimmed)
TTATAACGGGGCCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTGGATCAAGTCTGAGCATCCCCTCAACTGAAAGGAAATACATACA
CTTAAAGTCTGGAGAACATAAACATTTGGCACTTCAGAGAAGATATGCAATTACCTGGTCATCAATTTTGGCAGCTTTCATTTCATAGGAACTGACCATT
GTATTGGCATCTTCAACTGCTCTCTCTGTGATTTGACTTTCCCAAGCCAAACAATCCCGCTGTTGTCTTGCCCGTACTCCTTCTAAAACAAGCTGTAGAA
CAGGAAATAAACTAACCACAGTGTACAACATTGGCAGCAGTAGAAACCTGAGACACTAGAAATGCACAGACAGAACGTTTTTTGGAGTACTCTTGAAGCG
ATGTAAGGAAATTTGTGGATGCGAAACTCTAAAAACCCAACATATCCATGTAAAGCTACAGAAATGTGTCTAAAGTAGTCCTATAACAGGAACTAATTCT
GCAAACATCTTAACCATGAGGGAAAAAAAGAATAAAACAGGCATATAATGCACAACACAATGCTGGAATCTCCCCGGCATCAAAGCAGTGCGGGACAAAT
TAATGCCACATTCATTGTCAGTCTTACTGAGAATGGATAACAATGAGATTCAATCAAATAAAGCTTAAGAAAAGCAAACCAGGTGCTATTTTTTAAAAGT
GGTAATACTGGCCAATCCACTACCGATTAGCCAAGTGTTTTACCCTCCCCTGTTCAAAGATAAGTTGGGGGTCTGGAAATAAGATCTAACAACAGCTACC
AGGACGGAAAAACCTCTCACAGATTAGTGCATGAAAATCTAGAAAAA
>LE_HBa0127L04_T7_60055 (trimmed for quality and cloning vector)
TAGAGTCGACCTGCAGGCATGCAAGCTTCTGGATCAAGTCTGAGCATCCCCTCAACTGAAAGGAAATACATACACTTAAAGTCTGGAGAACATAAACATT
TGGCACTTCAGAGAAGATATGCAATTACCTGGTCATCAATTTTGGCAGCTTTCATTTCATAGGAACTGACCATTGTATTGGCATCTTCAACTGCTCTCTC
TGTGATTTGACTTTCCCAAGCCAAACAATCCCGCTGTTGTCTTGCCCGTACTCCTTCTAAAACAAGCTGTAGAACAGGAAATAAACTAACCACAGTGTAC
AACATTGGCAGCAGTAGAAACCTGAGACACTAGAAATGCACAGACAGAACGTTTTTTGGAGTACTCTTGAAGCGATGTAAGGAAATTTGTGGATGCGAAA
CTCTAAAAACCCAACATATCCATGTAAAGCTACAGAAATGTGTCTAAAGTAGTCCTATAACAGGAACTAATTCTGCAAACATCTTAACCATGAGGGAAAA
AAAGAATAAAACAGGCATATAATGCACAACACAATGCTGGAATCTCCCCGGCATCAAAGCAGTGCGGGACAAATTAATGCCACATTCATTGTCAGTCTTA
CTGAGAATGGATAACAATGAGATTCAATCAAATAAAGCTTAAGAAAAGCAAACCAGGTGCTATTTTTTAAAAGTGGTAATACTGGCCAATCCACTACCGA
TTAGCCAAGTGTTTTACCCTCC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.966366
Expected error %
0.156433
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L24367.1:218-930 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.16%) Frame 2
Cloning vector pTugAS (derived from E. coli JM105) beta-lactamase and repressor protein gene, complete cds
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0127L04
Clone
Clone name
hba-127l4
LE_HBa0127L04
P127L04
Clone type
BAC
Contamination
none
Estimated length
118,343
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found