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Clone Read LE_HBa0012D01_SP6_5904

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0012D01_SP6_5904
Primer
SP6
ID from Seq. Facility
LE_HBa_012_D01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
706 trimmed for vector and quality, 866 raw
Genbank Accession
DU025495
Sequence
>LE_HBa0012D01_SP6_5904 (raw untrimmed)
AAAGGCCTTCCTGTCATCTAGGGAATATTANANGAAATGCACAATAGTACATCTAAAAACCCTGCTTATGTCACAATTAATTATCACAAGTACAGTTCAC
TTATAGATCTCCAAATGGCTAGATTTGGTAACCTAATTGTGGTATACTATGTGCCACTTTAATACACTTCCCTTTTTAGCCTTACCCCAGAAAGAAGGAC
ACCTCCGTCTGAAAACTATTAAACCTTCTGACATGACTTATTGGGAGCTGAGACCCACTTGATCGCTCATTCTTTTATATAAAGAGAGAAAAGGCAAAGA
CCTGAGGAACTCTCATCACTCATTATTGAAAGGATAAGTTATGTACTTCGTATATATTTGGGTTACATGTCCAAGGTCTACTTTAATTTCTTTTGTTTAC
CGCGCAGTCTAACGCCTTCACACTAAATGGGACAGATTAGCAAAACTCATGTTCATTAGCACTCTGACATTCACGTCTGAACTTGTGTAAAGCATTACAC
TACAGCATACAACTAAATATAGATCACTGCAAGTTCAATAAGCACCTGGGCCACCACTCTATGTCCTCTATAATCAATTATCGCCATAGCTAGGTTGTAC
AGTCCATGGATATCAACTTCCTGATATGCTTTTGTGCCTTTCAAGTCATTATTTGCGCTTGCATAAGTGGCTTGCTCGCTTTCAGTCAACTGAGTTTCAC
CGGACACTTCAGGAGGACAATCCAAGATGATGTTTGCAGCTTCTACAACTGAGCCCACATGCTCATTTCCCTTGGAAACATCGGAGACTCCCTGTGGCAA
ATTATTGGTGGTTTTTCTCTGACAAATTACGTAAATAACCCTGGTACCCCTCAAATTTAAAATTCA
>LE_HBa0012D01_SP6_5904 (trimmed for quality and cloning vector)
ACAATAGTACATCTAAAAACCCTGCTTATGTCACAATTAATTATCACAAGTACAGTTCACTTATAGATCTCCAAATGGCTAGATTTGGTAACCTAATTGT
GGTATACTATGTGCCACTTTAATACACTTCCCTTTTTAGCCTTACCCCAGAAAGAAGGACACCTCCGTCTGAAAACTATTAAACCTTCTGACATGACTTA
TTGGGAGCTGAGACCCACTTGATCGCTCATTCTTTTATATAAAGAGAGAAAAGGCAAAGACCTGAGGAACTCTCATCACTCATTATTGAAAGGATAAGTT
ATGTACTTCGTATATATTTGGGTTACATGTCCAAGGTCTACTTTAATTTCTTTTGTTTACCGCGCAGTCTAACGCCTTCACACTAAATGGGACAGATTAG
CAAAACTCATGTTCATTAGCACTCTGACATTCACGTCTGAACTTGTGTAAAGCATTACACTACAGCATACAACTAAATATAGATCACTGCAAGTTCAATA
AGCACCTGGGCCACCACTCTATGTCCTCTATAATCAATTATCGCCATAGCTAGGTTGTACAGTCCATGGATATCAACTTCCTGATATGCTTTTGTGCCTT
TCAAGTCATTATTTGCGCTTGCATAAGTGGCTTGCTCGCTTTCAGTCAACTGAGTTTCACCGGACACTTCAGGAGGACAATCCAAGATGATGTTTGCAGC
TTCTAC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.972148
Expected error %
0.160075
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g52140.1 Score 90 E Value 1e-18 Identity 26.50% Align. Length 161bp (22.80%) Frame 3
68416.m05723 tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515: TPR Domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_467482.1| Score 101 E Value 2e-20 Identity 24.60% Align. Length 206bp (29.18%) Frame 3
eukaryotic translation initiation factor 3 subunit (eIF-3)-like [Oryza sativa (japonica cultivar-group)] dbj|BAD12895.1| eukaryotic translation initiation factor 3 subunit (eIF-3)-like [Oryza sativa (japonica cultivar-group)] dbj|BAD09184.1| eukaryotic translation initiation factor 3 subunit (eIF-3)-like [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB41334.1| Score 91 E Value 3e-17 Identity 28.20% Align. Length 155bp (21.95%) Frame 3
putative protein [Arabidopsis thaliana] pir||T49093 hypothetical protein F4F15.250 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0012D01
Clone
Clone name
hba-12d1
LE_HBa0012D01
P012D01
Clone type
BAC
Contamination
none
Estimated length
111,858
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found