Clone Read LE_HBa0122I16_SP6_57630

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0122I16_SP6_57630
Primer
SP6
ID from Seq. Facility
LE_HBa_122_I16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
137 trimmed for vector and quality, 842 raw
Sequence
>LE_HBa0122I16_SP6_57630 (raw untrimmed)
CACTCCGATATCATAGGACTTACCATTCCTAGCATAAAAGGTAGCCAAATGGACCACTCTGCTAAAGCATCTTGGGTATCATCCTTCACAGCTGAGGTAG
TCTCCCGTTTAAATCTCCGAACACAACCAGATTTCCTCTTTTTAGCTCCAGTACATCGATCAGACTGATAAAATTCTGCACCATTAACCTTAGGAAATCC
ATTTGTGAGACCTTTACCAGATATATCTACTGGGTTCCCATTAGTTGACGTTGCTCCTTTTAATTCAGTCAGCTGCAAATTCAACTCATTTTGCTCTCTC
TATCTCATACCTTTTCTATTTATCACACCATTCTCTTTGCTGTTCAATTCCGCACCATATTGTTCACAAAATGTGTTGCCTTCGTAATATTCTACGTGGT
GTGGAGGTGTATGCTACTTTTATTTCTCTTACTTCTCTAGTTGAGGGCTTTACTATTTTAGGATGTGTGTGTTGAGATATTTCCTTTGTTTGTTTTAATC
TGCTTACCACTCTTTGCCCGCCTGCCTAAACGTCCATCTTACTCATTTGTTATGTATCCCCTGTACTATCATATATTCAAAGCGGTAAATGTACCATATA
TTGTTTAATCACTAAGTTTTATATGATATCTCATCTCAAGTATGTCTTATGCCTCTCAATCGTGCTTATTTTTGTAGTTTTTTTAGTTTAAATATAGTTT
CTCTGAATATTAGAACATTGTCTGCTTCTATCTCTTAATGTTGCCTATTGGTTATTAATATTAATGGAACAAAACGTGATTTGTTTCAGAACTAGATATC
TTACGTTATCACGTATCGATAGTATCTGCGTTTTGTACCTTA
>LE_HBa0122I16_SP6_57630 (trimmed for quality and cloning vector)
GCTAAAGCATCTTGGGTATCATCCTTCACAGCTGAGGTAGTCTCCCGTTTAAATCTCCGAACACAACCAGATTTCCTCTTTTTAGCTCCAGTACATCGAT
CAGACTGATAAAATTCTGCACCATTAACCTTAGGAAA

This is probably not a useful sequence.

Problems:
  • Short cloning insert
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.969006
Expected error %
0.265581
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL25116.1| Score 96 E Value 3e-19 Identity 33.30% Align. Length 134bp (97.81%) Frame 1
heterochromatin protein 1-like protein [Lycopersicon esculentum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0122I16
Clone
Clone name
hba-122i16
LE_HBa0122I16
P122I16
Clone type
BAC
Contamination
none
Estimated length
103,950
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found