Clone Read LE_HBa0074L22_SP6_55482

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0074L22_SP6_55482
Primer
SP6
ID from Seq. Facility
LE_HBa_074_L22-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
808 trimmed for vector and quality, 840 raw
Sequence
>LE_HBa0074L22_SP6_55482 (raw untrimmed)
GCCTAGGTTATTAGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCG
TTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGG
CTCCCCTGTCATCAGGTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTT
GGTAAACAGTCGCCCGGGCCTGGTCACTGCGACCCCCTTTGTGAGGAGGCACCCCTTCTCCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGA
GTTGTCTCGCGCCCCTAGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACCCTCTTGCTTAACGTCGTTCGAGCTTTTCCTGGGAGTA
TGGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTATTCGAACATTGGCTCGAGGCATTTTCTCTACCCCTTCTTACCCTGAAAAAGCAGGGACACCTTAC
GTTCTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGGGACTAACAAGGGGTAGTACAGGAATATTCACCTGTTGTCCATCGACT
ACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTAGGTTTTCGGGGCATTGGATTCTCACCAATGTTT
GCGTTACTCAAGCCGACATTCTCGCTTCCGCTCGTCCACA
>LE_HBa0074L22_SP6_55482 (trimmed for quality and cloning vector)
TCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCG
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTCCCCTGTCATCAGG
TCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCG
GGCCTGGTCACTGCGACCCCCTTTGTGAGGAGGCACCCCTTCTCCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCGCGCCCCT
AGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACCCTCTTGCTTAACGTCGTTCGAGCTTTTCCTGGGAGTATGGCATGGGTTACTTC
AGCGCCGTAGCGCCTGGTATTCGAACATTGGCTCGAGGCATTTTCTCTACCCCTTCTTACCCTGAAAAAGCAGGGACACCTTACGTTCTTGAACCGATAA
CCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGGGACTAACAAGGGGTAGTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGA
TCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTAGGTTTTCGGGGCATTGGATTCTCACCAATGTTTGCGTTACTCAAGCCGA
CATTCTCG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.97872
Expected error %
0.262665
Sequence Annotations 
Automatic Annotations
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 159 E Value 5e-39 Identity 95.70% Align. Length 613bp (75.87%) Frame 2
Escherichia coli K12, complete genome
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 100 E Value 3e-22 Identity 90.60% Align. Length 295bp (36.51%) Frame 2
No sequence description found.
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 1562 E Value 0 Identity 99.40% Align. Length 807bp (99.88%) Frame 2
Nicotiana tabacum plastid, complete genome
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|ZP_00203428.1| Score 156 E Value 7e-37 Identity 27.10% Align. Length 272bp (33.66%) Frame 3
hypothetical protein Avar03000173 [Anabaena variabilis ATCC 29413]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|ZP_00327142.1| Score 107 E Value 3e-22 Identity 30.50% Align. Length 173bp (21.41%) Frame 3
hypothetical protein Tery02002583 [Trichodesmium erythraeum IMS101]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0074L22
Clone
Clone name
hba-74l22
LE_HBa0074L22
P074L22
Clone type
BAC
Contamination
none
Estimated length
66,463
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found