Clone Read LE_HBa0062M01_SP6_54720

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0062M01_SP6_54720
Primer
SP6
ID from Seq. Facility
LE_HBa_062_M01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
702 trimmed for vector and quality, 779 raw
Sequence
>LE_HBa0062M01_SP6_54720 (raw untrimmed)
CTTTGGAACTTTGAGGTGGTACAACTCCCAATGGATCCAGGTACAACACCACCATATTTAATATTCAAGTCCTTTACATATAGATGTCGAAACATATCTA
TATGTTGGAACCCCTTACGAAAATGTGGATCGAAGACCATAATTGACCTCAACTTTAAGTTAGAAAGATCAAGTGAGGGGTACCTTTGTCCTTGACTATG
AATGACATGTCGGATACATACAGAGGATATGGAGTGGCTTGTTGGATCATAAACGTCAAAGAAGTTTACCTCCAATGCCTTTTGTATCGCAAGATCATGG
AGTAAATCATGAACCCTACATTCAGTAACTTTTTCCCAAAATGTATTAGCCACTTGAACCAAGCTTCGTCTTATCAGCTCATTCAAGAAGCTTTCAGCGA
CATCCTCCATTCTTTCTCCTCTGGGTATGAAACCCTCTGCCATCCACAACCGTATTATGTTATCAGCCTCGACCACTTGATCTTCTGGAAAAATACCAAA
GTAGAGAAAACACTGCTTGAGCGCAGTTGACAAATCATTGTAGCTTAATGATAGTATGTTAGAGATTTCAATAGATTTATCTTCTTTAATGTTCTTCCAA
AGGTGATCTTTCACCTTTTGCCATTCGTTTAGCCCCTTTTTATGCGAAAGTAGTCCGCTCAATACAACAACTGCAAGAGGTAAGCCTCTACACTTTTCCA
CCATATCCCTAGCTAGACTTTTCCATTTCTGGAACCATTGCTCGAATATCAAGTAGTTTCCCAAGAAAGAGATCCCAAC
>LE_HBa0062M01_SP6_54720 (trimmed for quality and cloning vector)
CTCCCAATGGATCCAGGTACAACACCACCATATTTAATATTCAAGTCCTTTACATATAGATGTCGAAACATATCTATATGTTGGAACCCCTTACGAAAAT
GTGGATCGAAGACCATAATTGACCTCAACTTTAAGTTAGAAAGATCAAGTGAGGGGTACCTTTGTCCTTGACTATGAATGACATGTCGGATACATACAGA
GGATATGGAGTGGCTTGTTGGATCATAAACGTCAAAGAAGTTTACCTCCAATGCCTTTTGTATCGCAAGATCATGGAGTAAATCATGAACCCTACATTCA
GTAACTTTTTCCCAAAATGTATTAGCCACTTGAACCAAGCTTCGTCTTATCAGCTCATTCAAGAAGCTTTCAGCGACATCCTCCATTCTTTCTCCTCTGG
GTATGAAACCCTCTGCCATCCACAACCGTATTATGTTATCAGCCTCGACCACTTGATCTTCTGGAAAAATACCAAAGTAGAGAAAACACTGCTTGAGCGC
AGTTGACAAATCATTGTAGCTTAATGATAGTATGTTAGAGATTTCAATAGATTTATCTTCTTTAATGTTCTTCCAAAGGTGATCTTTCACCTTTTGCCAT
TCGTTTAGCCCCTTTTTATGCGAAAGTAGTCCGCTCAATACAACAACTGCAAGAGGTAAGCCTCTACACTTTTCCACCATATCCCTAGCTAGACTTTTCC
AT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.968163
Expected error %
0.321228
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g46730.1 Score 172 E Value 2e-43 Identity 15.60% Align. Length 614bp (87.46%) Frame 1
68416.m05073 disease resistance protein (CC-NBS class), putative domain signature CC-NBS exists, suggestive of a disease resistance protein.
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g46530.1 Score 162 E Value 2e-40 Identity 15.00% Align. Length 578bp (82.34%) Frame 1
68416.m05051 disease resistance protein, RPP13-like (CC-NBS class), putative domain signature CC-NBS exists, suggestive of a disease resistance protein. Closest homolog in Col-0 to RPP13.
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAS93947.1| Score 172 E Value 6e-42 Identity 15.20% Align. Length 650bp (92.59%) Frame 1
disease resistance protein RPP13 variant [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB51192.1| Score 172 E Value 1e-41 Identity 15.60% Align. Length 614bp (87.46%) Frame 1
putative protein [Arabidopsis thaliana] sp|Q9STE7|R13L3_ARATH Putative disease resistance RPP13-like protein 3 ref|NP_190257.1| disease resistance protein (CC-NBS class), putative [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0062M01
Clone
Clone name
hba-62m1
LE_HBa0062M01
P062M01
Clone type
BAC
Contamination
none
Estimated length
86,728
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found