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Clone Read LE_HBa0002P16_T7_53359

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0002P16_T7_53359
Primer
T7
ID from Seq. Facility
LE_HBa_002_P16-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
740 trimmed for vector and quality, 785 raw
Genbank Accession
DU078838
Sequence
>LE_HBa0002P16_T7_53359 (raw untrimmed)
GGCTCGGATCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTACCTTTCACTTAGGTACATACCAAACGTTGTTTGCGCCCTTTTTAACGC
TTGAAAATAAGTAAGAGAATCGCTTATTAATGCGTATAAATGTAGAGAGACGATTGAAGAGTACGCGGATGAAATAAAGAAGCTAAGCATGAGAGTATTG
GAAACGTTGGGTAAAGCTTTGGGGATTGATGAAGATGAAGTGAAGAGTGTTTTCAAAGAAGGAATGCAATCGATGAGGATGAATTACTATCCACCATGTC
CACAACCAGAGAAAGTGATGGGACTTACTCCTCATTCAGATGCAACAGGCCTTACAATACTTCTTCAAGTTAATGAAACTCAAGGTCTTCAAATTAAAAA
AGATGGAATTTGGATCCCCATTGAACCACTCCCTAATGCCTTCATAGTCAATGTTGGAGATGCTTTTGAGGTACTTCCTTCATCCTCTTCTCTCAATATT
TATTGAGTAGTATGGTATTATTTTTACCGTAGTTTTAATTTATGTGGTGATATTTGACTACGATCTTTTGTCAAAGGGGAGATATAACAAGTAACAAAAT
GAAGAGAAAAAACATCTCATGACTAGCTAGTTGAATATTCCCATAAATTTCCTTGGTATTAGTGTTTCAGTAGTCCATACTTATTTAGGAGTCTGCATTA
GCTATATCAATCGTACAATTTATTTAGGGAAAATGTAGAAGTACTTTTTTATCCTATGTTTGAAATCTAAAAGATTTAATTATAC
>LE_HBa0002P16_T7_53359 (trimmed for quality and cloning vector)
AGCTTTACCTTTCACTTAGGTACATACCAAACGTTGTTTGCGCCCTTTTTAACGCTTGAAAATAAGTAAGAGAATCGCTTATTAATGCGTATAAATGTAG
AGAGACGATTGAAGAGTACGCGGATGAAATAAAGAAGCTAAGCATGAGAGTATTGGAAACGTTGGGTAAAGCTTTGGGGATTGATGAAGATGAAGTGAAG
AGTGTTTTCAAAGAAGGAATGCAATCGATGAGGATGAATTACTATCCACCATGTCCACAACCAGAGAAAGTGATGGGACTTACTCCTCATTCAGATGCAA
CAGGCCTTACAATACTTCTTCAAGTTAATGAAACTCAAGGTCTTCAAATTAAAAAAGATGGAATTTGGATCCCCATTGAACCACTCCCTAATGCCTTCAT
AGTCAATGTTGGAGATGCTTTTGAGGTACTTCCTTCATCCTCTTCTCTCAATATTTATTGAGTAGTATGGTATTATTTTTACCGTAGTTTTAATTTATGT
GGTGATATTTGACTACGATCTTTTGTCAAAGGGGAGATATAACAAGTAACAAAATGAAGAGAAAAAACATCTCATGACTAGCTAGTTGAATATTCCCATA
AATTTCCTTGGTATTAGTGTTTCAGTAGTCCATACTTATTTAGGAGTCTGCATTAGCTATATCAATCGTACAATTTATTTAGGGAAAATGTAGAAGTACT
TTTTTATCCTATGTTTGAAATCTAAAAGATTTAATTATAC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.956552
Expected error %
0.452979
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g17020.1 Score 145 E Value 2e-35 Identity 17.40% Align. Length 356bp (48.11%) Frame 1
68414.m02067 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase [Petunia x hybrida][GI:311658], leucoanthocyanidin dioxygenase [Malus domestica][SP|P51091]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g25310.1 Score 139 E Value 1e-33 Identity 17.60% Align. Length 356bp (48.11%) Frame 1
68417.m03640 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase [Petunia x hybrida][GI:311658], [Solanum tuberosum][GI:1039356]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA55654.1| Score 145 E Value 1e-33 Identity 17.40% Align. Length 356bp (48.11%) Frame 1
SRG1 [Arabidopsis thaliana] gb|AAD50032.1| SRG1 Protein [Arabidopsis thaliana] gb|AAM98100.1| At1g17020/F6I1.30 [Arabidopsis thaliana] ref|NP_173145.1| oxidoreductase, 2OG-Fe(II) oxygenase family protein [Arabidopsis thaliana] gb|AAK82564.1| F6I1.30/F6I1.30 [Arabidopsis thaliana] pir||S44261 SRG1 protein - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB81342.1| Score 139 E Value 6e-32 Identity 17.60% Align. Length 356bp (48.11%) Frame 1
SRG1-like protein [Arabidopsis thaliana] emb|CAA23072.1| SRG1-like protein [Arabidopsis thaliana] ref|NP_194261.1| oxidoreductase, 2OG-Fe(II) oxygenase family protein [Arabidopsis thaliana] gb|AAS76252.1| At4g25310 [Arabidopsis thaliana] gb|AAR92265.1| At4g25310 [Arabidopsis thaliana] pir||T05552 SRG1 protein-related protein F24A6.150 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0002P16
Clone
Clone name
hba-2p16
LE_HBa0002P16
C02HBa0002P16
P002P16
Clone type
BAC
Contamination
none
Estimated length
66,292
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found