Clone Read LE_HBa0064O22_T7_49483

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0064O22_T7_49483
Primer
T7
ID from Seq. Facility
LE_HBa_064_O22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
620 trimmed for vector and quality, 795 raw
Sequence
>LE_HBa0064O22_T7_49483 (raw untrimmed)
TTAGTCGATTCTAGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCCAATACGCTAGCATAGTCCAATTGAGACTGACTTTCGCCTTTATTCT
TTGCAAGATGAAGATTTACATCAATTGGAGTCTTTGCCCTTTTAAAATTCAAAAATTTGAATTTTTCAAGTATCTTTTGAATATAATGAGTTTGAGACAA
TGCTAGACCGTTAGGAGTTTTAAGAATTTTAATTCCTAATATCACATCAGCAACTCCTAAATCTTTCATATCAAACTTACTAGCAAGCATACGCTTTGTA
GCTTTTATATTAGCAATTTCTTTGCTCATTATAAGCATATCATCTAAATATAGGCATAAAATAACTTCCTGATTTGGAGTATCTTTAATGTAAACACATT
TATCACATTCATTGATCTTAAATCCATTTGACAATATGGTTTGATCAAACTTTGCATGTCATTGTTTTGGTGCTTGTTTTAGCCCATAAAGTGACTTAAT
GAGTTTGCACACTTTCTTTTCTTTACCAGGAACTACAAAGCCCTCAGGATCTTCTATGTAAATTTCCTCTTCAAGCTCTCCATTTAAGAAGGTTGTTTTC
ACATCCATTTGATGAATTTCAAGACCGTATACTGCAGCTAGTGCAATTAACATCCGAATTGATGTAATTCTAGTCACGGGCGAGTATGTGTTAAAATAAT
CAAGAACTTCTTTTTGTCTAAACCCTTTGACAACAAGTCCTTTCTTTATATTTGGCAATAGTTCCCATCGTCTTTCATCTTCCTTTTTAAAAATC
>LE_HBa0064O22_T7_49483 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCCAATACGCTAGCATAGTCCAATTGAGACTGACTTTCGCCTTTATTCTTTGCAAGATGAAGATTTACA
TCAATTGGAGTCTTTGCCCTTTTAAAATTCAAAAATTTGAATTTTTCAAGTATCTTTTGAATATAATGAGTTTGAGACAATGCTAGACCGTTAGGAGTTT
TAAGAATTTTAATTCCTAATATCACATCAGCAACTCCTAAATCTTTCATATCAAACTTACTAGCAAGCATACGCTTTGTAGCTTTTATATTAGCAATTTC
TTTGCTCATTATAAGCATATCATCTAAATATAGGCATAAAATAACTTCCTGATTTGGAGTATCTTTAATGTAAACACATTTATCACATTCATTGATCTTA
AATCCATTTGACAATATGGTTTGATCAAACTTTGCATGTCATTGTTTTGGTGCTTGTTTTAGCCCATAAAGTGACTTAATGAGTTTGCACACTTTCTTTT
CTTTACCAGGAACTACAAAGCCCTCAGGATCTTCTATGTAAATTTCCTCTTCAAGCTCTCCATTTAAGAAGGTTGTTTTCACATCCATTTGATGAATTTC
AAGACCGTATACTGCAGCTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.941373
Expected error %
0.0680305
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65303.1:1-108 Score 66 E Value 4e-12 Identity 100.00% Align. Length 32bp (5.16%) Frame 2
Cloning vector pGEM-4
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M16728.1:1-87 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (5.00%) Frame 2
S.aureus multiple cloning site vector upstream of beta-lactamase gene, clone pWN1818
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 97 E Value 9e-21 Identity 10.00% Align. Length 548bp (88.39%) Frame 2
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89783.1| Score 327 E Value 2e-88 Identity 27.90% Align. Length 593bp (95.65%) Frame 2
putative retrovirus-related pol polyprotein-like [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38766.1| Score 261 E Value 1e-68 Identity 23.00% Align. Length 581bp (93.71%) Frame 2
putative polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0064O22
Clone
Clone name
hba-64o22
LE_HBa0064O22
P064O22
Clone type
BAC
Contamination
none
Estimated length
32,925
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found