Clone Read LE_HBa0010F15_T7_4877

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0010F15_T7_4877
Primer
T7
ID from Seq. Facility
LE_HBa_010_F15-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
766 trimmed for vector and quality, 1021 raw
Genbank Accession
DU024710
Sequence
>LE_HBa0010F15_T7_4877 (raw untrimmed)
TTAAACTGGTACCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTAGTCATCATATCGGAACCACCCTTATTTGTATGAATCTTTTCAAGAT
CAAGCAACTTAGAATCCTACACATCTCGAATCCAATGGTATCTCACATCAATGTGTTTAGACCGACCATGGAACGTAGAATTCTTGCCAAGATGTATAGC
ACTTCGACTGTCACAATAAATCACATACCTCTCTTGAGCACAACCAAGTTCCCCCAAAAATCTCTTCATCCAAATCAATTCTTTACAAGCTTCAACGACA
ACAATAAGCTCAGCTTTTGTAGTAGATAGAGCAACACATTTTTGTAACCTAGATTGCCAAGACACAGCTCCCCCTGCAAAAGTAACCAAGTACCCTGAAG
TAGACTTGCGAGTATCAACATCACCAGCCATGTCTGAATAAGTATAACCACAAAGAATAGGCTTCTCTATACCAAAACACAAACTCAGACTAGAAGTGCC
TTAGAGATATCTCATAACCCACTTCACAACATTCCAATGTTCTCTTCCCGGATTAGAAAGAAAATGGCTAACAACTACCAACAGCGTGAGCAATATCCGG
TCTTGTACAAACCATCGCATACATCAAACTACCAACAGCTGAAGCATAAGGAACTTTCTTCATATCTTCCTTCTCATCATCACTAGAAGGACACTGTTTC
GTGCTCACTTTGAAGTTCATAGCTAAAGGTGTACTGACAACCTTAGCTTTGTCAATGCTGAATCTGCGAAGTACTTTCTAAATGTACTTCTCTTGTGATA
ATACCCATTTCTTGCCTTTCTATCACGAACATCTGCATGCCAAGATCTGTCTTGCTGGTCCTAATTTTTTCTACCAAAAACTTACTCAATCTTTGCTCAA
CTTCGAGATCTGCAGTACTTTGACCCAAACAACCTTGTCTCCACTTAAACCAACCAAAAATAAAGTCCCCTCCAAAAACTTTTCCCAAACCAAATGGTCT
GAAAAATCTTTGGAACCCCGC
>LE_HBa0010F15_T7_4877 (trimmed for quality and cloning vector)
AGCTTTAGTCATCATATCGGAACCACCCTTATTTGTATGAATCTTTTCAAGATCAAGCAACTTAGAATCCTACACATCTCGAATCCAATGGTATCTCACA
TCAATGTGTTTAGACCGACCATGGAACGTAGAATTCTTGCCAAGATGTATAGCACTTCGACTGTCACAATAAATCACATACCTCTCTTGAGCACAACCAA
GTTCCCCCAAAAATCTCTTCATCCAAATCAATTCTTTACAAGCTTCAACGACAACAATAAGCTCAGCTTTTGTAGTAGATAGAGCAACACATTTTTGTAA
CCTAGATTGCCAAGACACAGCTCCCCCTGCAAAAGTAACCAAGTACCCTGAAGTAGACTTGCGAGTATCAACATCACCAGCCATGTCTGAATAAGTATAA
CCACAAAGAATAGGCTTCTCTATACCAAAACACAAACTCAGACTAGAAGTGCCTTAGAGATATCTCATAACCCACTTCACAACATTCCAATGTTCTCTTC
CCGGATTAGAAAGAAAATGGCTAACAACTACCAACAGCGTGAGCAATATCCGGTCTTGTACAAACCATCGCATACATCAAACTACCAACAGCTGAAGCAT
AAGGAACTTTCTTCATATCTTCCTTCTCATCATCACTAGAAGGACACTGTTTCGTGCTCACTTTGAAGTTCATAGCTAAAGGTGTACTGACAACCTTAGC
TTTGTCAATGCTGAATCTGCGAAGTACTTTCTAAATGTACTTCTCTTGTGATAATACCCATTTCTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.95753
Expected error %
0.678825
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 82 E Value 2e-16 Identity 10.00% Align. Length 449bp (58.62%) Frame 2
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T02206 Score 272 E Value 1e-100 Identity 71.40% Align. Length 762bp (99.48%) Frame 2
hypothetical protein - common tobacco retrotransposon Tto1 dbj|BAA11674.1| ORF(AA 1-1338) [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA32025.1| Score 241 E Value 4e-88 Identity 64.00% Align. Length 762bp (99.48%) Frame 2
unnamed protein product [Nicotiana tabacum] sp|P10978|POLX_TOBAC Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Contains: Protease ; Reverse transcriptase ; Endonuclease]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0010F15
Clone
Clone name
hba-10f15
LE_HBa0010F15
P010F15
Clone type
BAC
Contamination
none
Estimated length
123,431
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found