Clone Read LE_HBa0063H12_SP6_48358

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0063H12_SP6_48358
Primer
SP6
ID from Seq. Facility
LE_HBa_063_H12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
837 trimmed for vector and quality, 985 raw
Sequence
>LE_HBa0063H12_SP6_48358 (raw untrimmed)
AAAAAAAAGTGGTTTCCTNNNGCTAGTGAATCACCTCTCGAGAAATATCAACATACTTATGAGGTCATCTCAAGAATTTTTAAAAAACTTACAACTTTTT
CACCATCAGTGATACGAAATTAATGTTCATTTCTTATCATGATATCAGAGTCAGACTCATTAAACTGTTGGTTTATTAGGAGTCTAAAATAATCAAATAT
ATATAACACCATAATGCCACAACCAAAACAAAACTGGAAAAACACATCCTGTCATATGATATTTAAATGTAAGACAACTCCAGCTTTAATTTATAAAATA
GAATAGAGCTAAACTTTTAATGAGAAAAAACAATGATAATGTTCCCAGGAAGTCTTGTTACCTTAACAAAACAATAAAAATCCAAATACTATTTTGGTGC
AGGTAGACAACAATGGACCAATCATCTCACCTTAACTAGAAAATGTAAGTTCCAAAAACTACAATGTCTAGTAACAATCACTGGCAGAGCTGTTCTACTG
CAGTAACAATCTGGGGAGGTTGGACAACGGTCCAGTTCTCTAACGTCCCAGCATATGGCGTTGGCACATCCTGTGAGGACAGACATATGATTGGGGCATC
GAGATAGTCATGGAAGTTCTCGGTTATAGCAGCTGTCAAGCTGGCACCAATACCTCCTGTACGCATACATTCCTCGACAATGAGGACGCGATGAGTCTTC
TTCACTGAATTCCCGATGGTGTGAAGGTCAAACGGTTTCAGTGACCTGATATCAATGACCTCGGGATCATAACCCTTGTTCACGAGTGTCTTAGCAGCTT
GCATGACATGATACCTCATCCTGGAATAAGTTAGAATGGTAACATGCTCACCAGGGCGTACCATCTCTGCTTCTTTCAGATTCACACATACTCTTCGTCT
GGATTCTCTCCTTGAGGTGTAGAGCAAACATGCTCAAAAAAATCACAGGTTAATACTTCGAAAGCACCTCATCAGGCCTTGCGTG
>LE_HBa0063H12_SP6_48358 (trimmed for quality and cloning vector)
CTCTCGAGAAATATCAACATACTTATGAGGTCATCTCAAGAATTTTTAAAAAACTTACAACTTTTTCACCATCAGTGATACGAAATTAATGTTCATTTCT
TATCATGATATCAGAGTCAGACTCATTAAACTGTTGGTTTATTAGGAGTCTAAAATAATCAAATATATATAACACCATAATGCCACAACCAAAACAAAAC
TGGAAAAACACATCCTGTCATATGATATTTAAATGTAAGACAACTCCAGCTTTAATTTATAAAATAGAATAGAGCTAAACTTTTAATGAGAAAAAACAAT
GATAATGTTCCCAGGAAGTCTTGTTACCTTAACAAAACAATAAAAATCCAAATACTATTTTGGTGCAGGTAGACAACAATGGACCAATCATCTCACCTTA
ACTAGAAAATGTAAGTTCCAAAAACTACAATGTCTAGTAACAATCACTGGCAGAGCTGTTCTACTGCAGTAACAATCTGGGGAGGTTGGACAACGGTCCA
GTTCTCTAACGTCCCAGCATATGGCGTTGGCACATCCTGTGAGGACAGACATATGATTGGGGCATCGAGATAGTCATGGAAGTTCTCGGTTATAGCAGCT
GTCAAGCTGGCACCAATACCTCCTGTACGCATACATTCCTCGACAATGAGGACGCGATGAGTCTTCTTCACTGAATTCCCGATGGTGTGAAGGTCAAACG
GTTTCAGTGACCTGATATCAATGACCTCGGGATCATAACCCTTGTTCACGAGTGTCTTAGCAGCTTGCATGACATGATACCTCATCCTGGAATAAGTTAG
AATGGTAACATGCTCACCAGGGCGTACCATCTCTGCT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.966363
Expected error %
0.212141
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g30120.1 Score 257 E Value 6e-69 Identity 31.80% Align. Length 389bp (46.48%) Frame 1
68414.m03681 pyruvate dehydrogenase E1 component beta subunit, chloroplast identical to pyruvate dehydrogenase E1 beta subunit [Arabidopsis thaliana] GI:2454184; identical to cDNA pyruvate dehydrogenase E1 beta subunit mRNA, nuclear gene encoding plastid protein GI:2454183
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g34590.1 Score 254 E Value 3e-68 Identity 31.50% Align. Length 389bp (46.48%) Frame 1
68415.m04250 transketolase family protein similar to SP|O66113 Pyruvate dehydrogenase E1 component, beta subunit (EC 1.2.4.1). {Zymomonas mobilis}; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF02780: Transketolase, C-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD94262.1| Score 257 E Value 3e-67 Identity 31.80% Align. Length 389bp (46.48%) Frame 1
hypothetical protein [Arabidopsis thaliana] gb|AAM16249.1| At1g30120/T2H7_8 [Arabidopsis thaliana] gb|AAK32751.1| At1g30120/T2H7_8 [Arabidopsis thaliana] ref|NP_174304.1| pyruvate dehydrogenase E1 component beta subunit, chloroplast [Arabidopsis thaliana] gb|AAG50862.1| pyruvate dehydrogenase E1 beta subunit, putative [Arabidopsis thaliana] pir||C86425 probable pyruvate dehydrogenase E1 beta subunit - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB86804.1| Score 257 E Value 3e-67 Identity 31.80% Align. Length 389bp (46.48%) Frame 1
pyruvate dehydrogenase E1 beta subunit [Arabidopsis thaliana] gb|AAM65270.1| pyruvate dehydrogenase E1 beta subunit, putative [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0063H12
Clone
Clone name
hba-63h12
LE_HBa0063H12
P063H12
Clone type
BAC
Contamination
none
Estimated length
21,380
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found