Clone Read LE_HBa0059P13_SP6_47208

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0059P13_SP6_47208
Primer
SP6
ID from Seq. Facility
LE_HBa_059_P13-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
747 trimmed for vector and quality, 851 raw
Sequence
>LE_HBa0059P13_SP6_47208 (raw untrimmed)
GTTTAAGAGTTAAATTTCTGTAACAAGGCATATTCACAATGTGCTACTANNTTGCCAGCGGTGACTATAAAAATACACATGTATTTATTTTGAGAATACC
ATTATTAGCATCAGCAGATGAAAAATTACCTATTGCTTTTAAAGGAACACGGTTCCCAATTCGATTATGTTTTGCAATGACAACAAACAAATCTCAAGGT
CAAACATTAGATTTCGTAAGAATCTATTTGCGCGAACCAGTTTTTTTCACATGGTCAACTTTATGTTGCTTTTTCCAGAACTAAAACTTTTAAAAATGTC
AAAATACTAATATGTACGCCTACAATTGAACATACAAATGACCATTTCATGTATAATATAGTCTACAAAGAAATCATTCAAAAAGCATTGACCTAAATTC
CTAAATAATCTATCCTTCATAAGCCAAATCTGTTGTTGCAAGCTCCATAGATCAAGAAATGCCTATGATATTCATGATTGGTGACACTTTTTTAATTAAA
TAGAGATTTTCTTAATGCAACTCATGCTAATATGTTTTGGGACTTTTAACCAATTGTTGATCAGCCCTTTATTTTTAATGTCTTGGTAAAATAGTGAATG
CCACAATTTAGGTGATGCAAAGAGCTGCAGATGAGCCTGTTGATAAATTGTATACTTCGTCTATAATGACTGATGCCATTCTCCAGGTAAAATGACGCAT
CATTTCATTATGAGATTTTTCTTTATTTTGTTTTCTACCAATCTCTATATTGTTTCAGTTGCAAATGCACATTATGGCAATATAGATGTATGTCCACCTG
CAAAACCTTTTTTCAATTTCAACCTCAAGATAACAATCTTAATGGCTATAG
>LE_HBa0059P13_SP6_47208 (trimmed for quality and cloning vector)
GCCAGCGGTGACTATAAAAATACACATGTATTTATTTTGAGAATACCATTATTAGCATCAGCAGATGAAAAATTACCTATTGCTTTTAAAGGAACACGGT
TCCCAATTCGATTATGTTTTGCAATGACAACAAACAAATCTCAAGGTCAAACATTAGATTTCGTAAGAATCTATTTGCGCGAACCAGTTTTTTTCACATG
GTCAACTTTATGTTGCTTTTTCCAGAACTAAAACTTTTAAAAATGTCAAAATACTAATATGTACGCCTACAATTGAACATACAAATGACCATTTCATGTA
TAATATAGTCTACAAAGAAATCATTCAAAAAGCATTGACCTAAATTCCTAAATAATCTATCCTTCATAAGCCAAATCTGTTGTTGCAAGCTCCATAGATC
AAGAAATGCCTATGATATTCATGATTGGTGACACTTTTTTAATTAAATAGAGATTTTCTTAATGCAACTCATGCTAATATGTTTTGGGACTTTTAACCAA
TTGTTGATCAGCCCTTTATTTTTAATGTCTTGGTAAAATAGTGAATGCCACAATTTAGGTGATGCAAAGAGCTGCAGATGAGCCTGTTGATAAATTGTAT
ACTTCGTCTATAATGACTGATGCCATTCTCCAGGTAAAATGACGCATCATTTCATTATGAGATTTTTCTTTATTTTGTTTTCTACCAATCTCTATATTGT
TTCAGTTGCAAATGCACATTATGGCAATATAGATGTATGTCCACCTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.932492
Expected error %
0.0499291
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g30420.1 Score 60 E Value 1e-14 Identity 50.80% Align. Length 306bp (40.96%) Frame 2
68416.m03847 hypothetical protein low similarity to SP|Q9UUA2 DNA repair and recombination protein pif1, mitochondrial precursor {Schizosaccharomyces pombe}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g54430.1 Score 59 E Value 4e-14 Identity 52.70% Align. Length 294bp (39.36%) Frame 2
68414.m06209 hypothetical protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_917837.1| Score 72 E Value 1e-15 Identity 61.30% Align. Length 318bp (42.57%) Frame 2
B1158C05.18 [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP20844.1| Score 70 E Value 4e-13 Identity 59.70% Align. Length 318bp (42.57%) Frame 2
expressed protein [Oryza sativa (japonica cultivar-group)] ref|XP_468746.1| unknown protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0059P13
Clone
Clone name
hba-59p13
LE_HBa0059P13
P059P13
Clone type
BAC
Contamination
none
Estimated length
67,237
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found