Clone Read LE_HBa0241K10_T7_402835

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0241K10_T7_402835
Primer
T7
ID from Seq. Facility
LE_HBa_241_K10-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
841 trimmed for vector and quality, 860 raw
Sequence
>LE_HBa0241K10_T7_402835 (raw untrimmed)
CGGGGTCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTCACCACCTCAAGGAGACTTACTGACTAATATGGATGACAAGAAACCAATTTTTCACTATATT
CTATGTAAACGTCGAAAAAAGGGGCAACCACAGCTACAGGAATGCACTCAAAAAGTCCAACCACTGAGGAAGGAACTTAGTCACATAACATTCCAAGATT
TGAAGGAGAAAATGACCGTCCCAATTGCACAAGTCTCATGTATCGCTTTGGAGCCTCCAAAGGCAATAGGCAAGTTGGTAAGATAAAAGAGAACTTTGAT
CAAAAGTTCTTCACATTATTTGAATAATCAGGCTATAATTTCTCAAATCCAGCGAATTTAGGAGATCTCAAGGATGATTTCACTGGTGAAAAGATACACA
GGCTTGCAAAGTCTCAAATGGAGTTGAGAAAGCAATGATATCATGTCGCTACTTATAAGTTTGGGTTAGGATTAAACTTGTCGGATCCTCTTTGGATTTC
GTCTAAGAAGGGGAAAGAAATAACTTCATCTCACTACACCTCTACAGAAATACAGAGGAATAACACCATGACAGACTTCAGTGTTTGAATGCATCAAAAG
ACTAACACCCTATGTTTCTGCATTGGAAAGGTTAGGCTGCAAAGATGAAAGAGAGTTATCTAAACAAGTGGACGAATATGCCACCATCTCAAATACCTCT
GTATTTCATCGCTTGGGAAACAAAAGAAAGTCATTATCCGAAAGGAGGTTGCTGGAGCATAAAAATCAAGAATCTTGTGATGTAATTGACGANCAAGAGA
TTCATAATGTTATTCCATCACGTATGAAGATAAAGTCTGTATTGTTGATCACCGCTGATG
>LE_HBa0241K10_T7_402835 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTCACCACCTCAAGGAGACTTACTGACTAATATGGATGACAAGAAACCAATTTTTCACTATATTCTATGT
AAACGTCGAAAAAAGGGGCAACCACAGCTACAGGAATGCACTCAAAAAGTCCAACCACTGAGGAAGGAACTTAGTCACATAACATTCCAAGATTTGAAGG
AGAAAATGACCGTCCCAATTGCACAAGTCTCATGTATCGCTTTGGAGCCTCCAAAGGCAATAGGCAAGTTGGTAAGATAAAAGAGAACTTTGATCAAAAG
TTCTTCACATTATTTGAATAATCAGGCTATAATTTCTCAAATCCAGCGAATTTAGGAGATCTCAAGGATGATTTCACTGGTGAAAAGATACACAGGCTTG
CAAAGTCTCAAATGGAGTTGAGAAAGCAATGATATCATGTCGCTACTTATAAGTTTGGGTTAGGATTAAACTTGTCGGATCCTCTTTGGATTTCGTCTAA
GAAGGGGAAAGAAATAACTTCATCTCACTACACCTCTACAGAAATACAGAGGAATAACACCATGACAGACTTCAGTGTTTGAATGCATCAAAAGACTAAC
ACCCTATGTTTCTGCATTGGAAAGGTTAGGCTGCAAAGATGAAAGAGAGTTATCTAAACAAGTGGACGAATATGCCACCATCTCAAATACCTCTGTATTT
CATCGCTTGGGAAACAAAAGAAAGTCATTATCCGAAAGGAGGTTGCTGGAGCATAAAAATCAAGAATCTTGTGATGTAATTGACGANCAAGAGATTCATA
ATGTTATTCCATCACGTATGAAGATAAAGTCTGTATTGTTG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.9687
Expected error %
0.379865
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M19092.1:1-90 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (3.80%) Frame 2
Cloning vector pJRD179 DNA
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L08913.1:221-309 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (3.80%) Frame 2
pIC20R cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0241K10
Clone
Clone name
hba-241k10
LE_HBa0241K10
P241K10
Clone type
BAC
Contamination
none
Estimated length
67,562
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found