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Clone Read LE_HBa0238I01_T7_398790

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0238I01_T7_398790
Primer
T7
ID from Seq. Facility
LE_HBa_238_I01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
381 trimmed for vector and quality, 865 raw
Sequence
>LE_HBa0238I01_T7_398790 (raw untrimmed)
NNNGGCGTCCTTAGAGCTCNACGGCCGCAGTGTGATGGATATCTGCAGAATTCGCCCTTAGAGTTTGATCCTGGCTCAAATTGAACGCTGGCGGCGTGCT
TTACACATGCACTTCGAACGGTAGCTCGGGCTTCGGCCTGGTGGCGAGTGGCGAACGGGTGATTAATATTTCGGAACGTACCCCATAGTGGGGGATAACT
GTATCGAAAATTACGCTAATACCGCATACGATCTACCGTATGAAAGCAGGGGACCGCAAGGCCTTGTGCTCCTGGAGCGGCCGATATCTGATTAGCTAGT
TGGTGAGGTAAAGGCTCACCTAGGCGACCATCAATAGCTGGTCTGAGAGGACGACCAGCCACACTGGAACTGATACACGGTCCAGACTCCTACGGGAGGC
ATCAGTGGGGAATTTTGGACTATGGGCGCAAGCCTGATCCAACAATGCCTCGTGAGTGAAGAAGGCCTTCCGGTTGTACAGCTCTTTTGTCAGGGAAGAA
ACCGCACTGGCTAATATCCACTGCTAATGACTGTACCTGAACAATAAGCACCGGCTAACTACGTGCCAGCATCCGCGGTAATACTTAGGGTGCAAGCGTT
AATCGGAATTACTGGGCGTATAGCGTGCGCATGCGGTTTTGTAAGTCTGTCGTGAAAGCCCCGGGCTCAACCTGGGAATTGCGATGGAGACTGCCAGGCT
TTGATCTGGCAGAAGGGGGTAGAATTTCCCGTGTAACCGTGAAATGCCTTGAGATTTGGAAGAACCCCCATGGCCAAAGGCCCCCCCCCTGGGTTAAGAT
TGACCCTTCTGCACCAATGCCTTGGGGCACAAACAGGATTTGATCCCCGGGGAATTCCCCCCCCA
>LE_HBa0238I01_T7_398790 (trimmed for quality and cloning vector)
GTATGAAAGCAGGGGACCGCAAGGCCTTGTGCTCCTGGAGCGGCCGATATCTGATTAGCTAGTTGGTGAGGTAAAGGCTCACCTAGGCGACCATCAATAG
CTGGTCTGAGAGGACGACCAGCCACACTGGAACTGATACACGGTCCAGACTCCTACGGGAGGCATCAGTGGGGAATTTTGGACTATGGGCGCAAGCCTGA
TCCAACAATGCCTCGTGAGTGAAGAAGGCCTTCCGGTTGTACAGCTCTTTTGTCAGGGAAGAAACCGCACTGGCTAATATCCACTGCTAATGACTGTACC
TGAACAATAAGCACCGGCTAACTACGTGCCAGCATCCGCGGTAATACTTAGGGTGCAAGCGTTAATCGGAATTACTGGGCG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.989261
Expected error %
1.71891
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 139 E Value 7e-35 Identity 87.40% Align. Length 331bp (86.88%) Frame 3
Nicotiana tabacum plastid, complete genome
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 88 E Value 5e-19 Identity 86.10% Align. Length 107bp (28.08%) Frame 3
No sequence description found.
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 224 E Value 4e-59 Identity 91.20% Align. Length 328bp (86.09%) Frame 3
Escherichia coli K12, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0238I01
Clone
Clone name
hba-238i1
LE_HBa0238I01
P238I01
Clone type
BAC
Contamination
none
Estimated length
98,012
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found