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Clone Read LE_HBa0117F20_T7_39831

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0117F20_T7_39831
Primer
T7
ID from Seq. Facility
LE_HBa_117_F20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
805 trimmed for vector and quality, 918 raw
Genbank Accession
DU087079
Sequence
>LE_HBa0117F20_T7_39831 (raw untrimmed)
CTTTAACTCGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGATGCCAAGATAGACACTGAAACCGGGACAGTTACAATGGAACCAAA
TCAGCCTAATGTGTAAGTTTATATACAGATGTACCTTATCGAAATGTTCAATGCGTTGACCCATTTTAGCATACACCTGATGTTTATGAGCATTGTCATG
TTCAGGTACGAGCAGCTCATCGATCACACCAAGCCCCTTTCTGGACGCACGTACAAATTAGTCAGTCAGCTTCTTGAACATGCACAAACACAACTTGCTA
GATAGTTTGGTTGAGGACATTAAGCTCGTGCCAATTTTGCATTGCTCCCGTACCTGATTAACACCTTTATATTCAAGAATGTTGCTTATGTTAATTCCTG
GATTTCCCTTGGAGGAAATTTTGAGATACATGATGAATTATACATTAGTTTCTTTTTTATCTTGTGTTGTCTCCATTGGAAGTGGTATGCCTTTGCATTT
TAGTACTCGTTTTTGCCATGAAAAAATTTGTTTTAGCTGGAATTTGCCAGGGCATTTTGTTTCATATGATGCTTTACTCTTCGCTCGTGATCACTTAGAG
CCCTCTGTTCATCAATAGGTACATAATAAATCTAGTGTCCATTACAAGGCGATGTCAATGCATTATTTTTGGTTAAATATTTTGGAAAATCAATTCATCT
ATAGATGACAAATTCAAAAAATGAAGTTTATAAGTGACGTTTCATATTTATCGTCTTCCTACCCTCCAATACATCTCATCTTCAAGTTGGATAGCATTTA
TATGATACGATTTGCTCGTCTACCGGGATAGGGGAAAGGAAAATGTACCATTGTTTATCGAAATTCTTCACCTATTACCAAATTTTCATACATATAGGGA
TGAAAATGGGATGGAAGG
>LE_HBa0117F20_T7_39831 (trimmed for quality and cloning vector)
AGCTTGATGCCAAGATAGACACTGAAACCGGGACAGTTACAATGGAACCAAATCAGCCTAATGTGTAAGTTTATATACAGATGTACCTTATCGAAATGTT
CAATGCGTTGACCCATTTTAGCATACACCTGATGTTTATGAGCATTGTCATGTTCAGGTACGAGCAGCTCATCGATCACACCAAGCCCCTTTCTGGACGC
ACGTACAAATTAGTCAGTCAGCTTCTTGAACATGCACAAACACAACTTGCTAGATAGTTTGGTTGAGGACATTAAGCTCGTGCCAATTTTGCATTGCTCC
CGTACCTGATTAACACCTTTATATTCAAGAATGTTGCTTATGTTAATTCCTGGATTTCCCTTGGAGGAAATTTTGAGATACATGATGAATTATACATTAG
TTTCTTTTTTATCTTGTGTTGTCTCCATTGGAAGTGGTATGCCTTTGCATTTTAGTACTCGTTTTTGCCATGAAAAAATTTGTTTTAGCTGGAATTTGCC
AGGGCATTTTGTTTCATATGATGCTTTACTCTTCGCTCGTGATCACTTAGAGCCCTCTGTTCATCAATAGGTACATAATAAATCTAGTGTCCATTACAAG
GCGATGTCAATGCATTATTTTTGGTTAAATATTTTGGAAAATCAATTCATCTATAGATGACAAATTCAAAAAATGAAGTTTATAAGTGACGTTTCATATT
TATCGTCTTCCTACCCTCCAATACATCTCATCTTCAAGTTGGATAGCATTTATATGATACGATTTGCTCGTCTACCGGGATAGGGGAAAGGAAAATGTAC
CATTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.963942
Expected error %
0.45289
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g57290.1 Score 70 E Value 2e-12 Identity 16.30% Align. Length 251bp (31.18%) Frame 1
68416.m06377 eukaryotic translation initiation factor 3E / eIF3e (TIF3E1) identical to eukaryotic initiation factor 3E subunit [Arabidopsis thaliana] gi|12407658|gb|AAG53613
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL85088.1| Score 70 E Value 9e-11 Identity 16.30% Align. Length 251bp (31.18%) Frame 1
putative translation initiation factor 3 [Arabidopsis thaliana] gb|AAK64017.1| putative translation initiation factor 3 [Arabidopsis thaliana] gb|AAG53613.1| eukaryotic initiation factor 3E subunit [Arabidopsis thaliana] ref|NP_567047.1| eukaryotic translation initiation factor 3E / eIF3e (TIF3E1) [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB68135.1| Score 70 E Value 9e-11 Identity 16.30% Align. Length 251bp (31.18%) Frame 1
translation initiation factor 3-like protein [Arabidopsis thaliana] pir||T45807 translation initiation factor 3-like protein - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0117F20
Clone
Clone name
hba-117f20
LE_HBa0117F20
P117F20
Clone type
BAC
Contamination
none
Estimated length
48,153
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found