Clone Read LE_HBa0234F06_T7_392086

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0234F06_T7_392086
Primer
T7
ID from Seq. Facility
LE_HBa_234_F06-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
112 trimmed for vector and quality, 1100 raw
Sequence
>LE_HBa0234F06_T7_392086 (raw untrimmed)
GGTCTACGGCGCAGTGTGATGGTATCTGCAGAATTCGCCCTTGGTTACCTTGTTACGACTTCACCCCAGTCACGAATCCTACCGTGGTAAGCGCCCTCCT
TGCGGTTAAGCTACCTACTTCTGGTAAAACCCGCCCCCATGGTGTGACGGGCGGTGAGTACAAGACCCGGGAACGTATTCACCGCGACATGCTGATCCGA
GATTACTATCTATTCCTACTTCATGGAGTCAAGTTGCAGACATCTATCCGGACTACTATACACTTTCTGGGATTTGCTCCCCCTCGCGGGTGGGCGGCCC
TCTGTATGTACCAATGAATGACTTGTGAATCCCTACCCATTAGTGACTTGAAGGACTAGACTTCTTGCAACCATTCCTCTCGTTTGTTGCCCGACGTGCT
CACTTATATTGGCGTATTCTTAATAAACTATTTTCAAAGTTGGTCTCTCCACATCTGGTATTATGATCCAATATTCTTATTACAATCAGTAAGACTAGCA
CCCTAGGTAATACTACTGTGATGACAGGCTTCCTTTATTCTTGCATCTAAACCGAAACGTCACTATAGGCCCTTCTGTCTATGTTCTATGTGTCTGCCTA
AATGTGTTAGATTATCTATTCGTGTCTGAACATTGTTCTGTCAACTTATATTCTTATCCTTGCTATTGAGTTTCCACTGGATGAATCCTCCATTTAATGT
TTTAATACTTTCGTTTCCTATTTTTTTCTTTGCAGAGAGTGTCTAGGGTTCAACGTCATGTCCTCTGTTTTTTTTACGTTATCGTCTTACTTGAGTTTTC
TCTATATTAGATAGTAACAATATTATGATGCGCTTTTATATTTTTCCTATTGAGTCGACTGCCCTGATTAGTTTTCCCCGCAGGTACACTGTCTTTTTCT
GAAGCGTTTCTATGGATGTACTCTAATGGATAATTATCGGTTGAATTTTGTGTTATTCACTTCGGGACATTATTCAAGCTTACTACTCTCATCGTTGTGG
GTGTCTCTCTCATTTCTCCTCTTTTGTCCATAGTCTTTTGTATTGTTTTATATATTACAGAACTCTTCTATGAATTTGTATTTTATATCCTATTATGTTG
>LE_HBa0234F06_T7_392086 (trimmed for quality and cloning vector)
AATTCGCCCTTGGTTACCTTGTTACGACTTCACCCCAGTCACGAATCCTACCGTGGTAAGCGCCCTCCTTGCGGTTAAGCTACCTACTTCTGGTAAAACC
CGCCCCCATGGT

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.919435
Expected error %
1.18534
Sequence Annotations 
Automatic Annotations
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 88 E Value 2e-18 Identity 88.70% Align. Length 79bp (70.54%) Frame 1
Escherichia coli K12, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0234F06
Clone
Clone name
hba-234f6
LE_HBa0234F06
P234F06
Clone type
BAC
Contamination
none
Estimated length
78,051
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found