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Clone Read LE_HBa0111J02_SP6_36146

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0111J02_SP6_36146
Primer
SP6
ID from Seq. Facility
LE_HBa_111_J02-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
589 trimmed for vector and quality, 765 raw
Genbank Accession
DU088500
Sequence
>LE_HBa0111J02_SP6_36146 (raw untrimmed)
CTTCTCCCACGGTTAGTTCTCACATCACGGAGCTCAGCAGCAGAGATGAACCCATTCTGGTCCTTGTCAAAACACTCTGAATGCCTCCTTCAGCTCCTCC
TCTGAGTCTGTATCCTTCATCTTCCTGGCCATGAGATTCAAAAACTCTGGGAAGTCGATGGTTCCATTACCATCAGCATCCACTTCAGTTATCATGTCCT
GGTGCTCGGCGCAGCTCAGTTGGGTTCTGTCCCAACGACCTCATCACAGTCCCAAGCTCCTTAGTTGTAATGCAACCTATTCAAACCAACAATATGAGCA
AACTAAACCAGAAGATCAAACAACGTATGCTTCTGACTGCATCTTTTACCAGTGCATACTTTCAAAAGAAACCAAATGTAAAGTTAACCATACCAGATAA
TATCACCTAGTGTTTCAATTTGAACATGAGAACTCGTGATTCTCAACCCACGTCATTGACCACTAGGCCAAGGATTTTGCTTTATTAAAGTAAAAAAACA
CAAGTACCATTTTAAATGCTTGTGAACTAATACAGAATCTGCAGGAGATCTACTCATACACGGCTACAATAAAGACAGAGAACCTATAACTACCATTTAA
AGCAAGTTTATATCATTATCTCAATAGACAGGAACTCTGGGCCCACATTAAAAAAAATTAATTCAAATACTAATTTCCCCATTACGGGGTCAAGGATTCA
CCAGTGTTTACAATAAAAGAAAGAAAATTTCTACTCACAAGCTGTTCAATCACTTACACTGAATG
>LE_HBa0111J02_SP6_36146 (trimmed for quality and cloning vector)
GGAGCTCAGCAGCAGAGATGAACCCATTCTGGTCCTTGTCAAAACACTCTGAATGCCTCCTTCAGCTCCTCCTCTGAGTCTGTATCCTTCATCTTCCTGG
CCATGAGATTCAAAAACTCTGGGAAGTCGATGGTTCCATTACCATCAGCATCCACTTCAGTTATCATGTCCTGGTGCTCGGCGCAGCTCAGTTGGGTTCT
GTCCCAACGACCTCATCACAGTCCCAAGCTCCTTAGTTGTAATGCAACCTATTCAAACCAACAATATGAGCAAACTAAACCAGAAGATCAAACAACGTAT
GCTTCTGACTGCATCTTTTACCAGTGCATACTTTCAAAAGAAACCAAATGTAAAGTTAACCATACCAGATAATATCACCTAGTGTTTCAATTTGAACATG
AGAACTCGTGATTCTCAACCCACGTCATTGACCACTAGGCCAAGGATTTTGCTTTATTAAAGTAAAAAAACACAAGTACCATTTTAAATGCTTGTGAACT
AATACAGAATCTGCAGGAGATCTACTCATACACGGCTACAATAAAGACAGAGAACCTATAACTACCATTTAAAGCAAGTTTATATCATT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.952708
Expected error %
0.137833
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g21274.1 Score 92 E Value 1e-30 Identity 95.70% Align. Length 247bp (41.94%) Frame 3
68418.m02533 calmodulin-6 (CAM6) identical to calmodulin-6 SP:Q03509 from [Arabidopsis thaliana]; contains Pfam profile: PF00036 EF hand
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g27030.2 Score 92 E Value 5e-24 Identity 95.70% Align. Length 214bp (36.33%) Frame 3
68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical to calmodulin GI:474183 from [Arabidopsis thaliana], SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
sp|P27164|CALM3_PETHY Score 92 E Value 5e-29 Identity 95.70% Align. Length 247bp (41.94%) Frame 3
Calmodulin-related protein gb|AAA33705.1| calmodulin-related protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_850097.1| Score 92 E Value 5e-29 Identity 95.70% Align. Length 247bp (41.94%) Frame 3
calmodulin-2/3/5 (CAM5) (TCH1) [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0111J02
Clone
Clone name
hba-111j2
LE_HBa0111J02
C02HBa0111J02
P111J02
Clone type
BAC
Contamination
none
Estimated length
113,243
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found