Clone Read LE_HBa0110E09_SP6_35152

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0110E09_SP6_35152
Primer
SP6
ID from Seq. Facility
LE_HBa_110_E09-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
548 trimmed for vector and quality, 833 raw
Sequence
>LE_HBa0110E09_SP6_35152 (raw untrimmed)
TTGGTATTTTGTTTTTTTGGTATTCTTGTGATACCAGATAGAAAGCAATATTGTCCGATGCCCGGATCCGGAATATGCAGAGAAGATGATTGCTGCTATT
GATGCTGTACGAGTGAGAGGAGACTCTGTTGGTGGTGTTGTAACTTGCATTGTTAGAAATCTCCCACGGGTAAGCTATCTCTGTGTTTGTAACTTGGAAA
TGTGATATGTATATCTGCACCAACATGAAATTTCATCTCAAATTGTAAAATACATGCCTGAGACTTTGTTAATGTTTACTAGGGTCTTGGTACACCAGTC
TTCGATAAACTTGAAGCTGAGCTAGCCAAAGCTTGCATGTCATTACCTGCGACAAAGGGTTTTGAGTTTGGCAGTGGCTTTGCAGGTACATTATCTTTAC
TTCACTGTTCCGGAGATGTAGCTTAACTTTATCACTGAAGGACTTGGCATGTTTTCTCTAATGCATTTTCCATGAGCATTGCTAGTCCTAAGCAGATAAA
CATTCCTCAAGATGTTCTAAGAAAGGGTGATTCTTTTCAACTGACATGAACTCCTTCAAGAATTATATTATAAAACTAGTAATATAGTAGTAATCATTTT
TCTGTTGCTGTCAAATGTCCAGTTATGGACTTTTGACACGGAAAGTTTTCTGTTTTTGATATATGATTATGGGCCATTAATTCCTATAAAAATCGATATT
CTCCTGTCACTATTTAAAAAATTTTTTTAAAAGTAAAGAAACATTCTTAAAATTTTTGTTTTTGATTATTCATCTTTTTTATTTGTTATTCTAAAAAAAT
AAGTGATGTGTTTCACGTATGAGTGGTCGACCG
>LE_HBa0110E09_SP6_35152 (trimmed for quality and cloning vector)
GATACCAGATAGAAAGCAATATTGTCCGATGCCCGGATCCGGAATATGCAGAGAAGATGATTGCTGCTATTGATGCTGTACGAGTGAGAGGAGACTCTGT
TGGTGGTGTTGTAACTTGCATTGTTAGAAATCTCCCACGGGTAAGCTATCTCTGTGTTTGTAACTTGGAAATGTGATATGTATATCTGCACCAACATGAA
ATTTCATCTCAAATTGTAAAATACATGCCTGAGACTTTGTTAATGTTTACTAGGGTCTTGGTACACCAGTCTTCGATAAACTTGAAGCTGAGCTAGCCAA
AGCTTGCATGTCATTACCTGCGACAAAGGGTTTTGAGTTTGGCAGTGGCTTTGCAGGTACATTATCTTTACTTCACTGTTCCGGAGATGTAGCTTAACTT
TATCACTGAAGGACTTGGCATGTTTTCTCTAATGCATTTTCCATGAGCATTGCTAGTCCTAAGCAGATAAACATTCCTCAAGATGTTCTAAGAAAGGGTG
ATTCTTTTCAACTGACATGAACTCCTTCAAGAATTATATTATAAAACT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.969974
Expected error %
0.0322916
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g48850.1 Score 77 E Value 3e-27 Identity 66.70% Align. Length 355bp (64.78%) Frame 1
68414.m05469 chorismate synthase, putative / 5-enolpyruvylshikimate-3-phosphate phospholyase, putative similar to chorismate synthase from Lycopersicon esculentum [SP|Q42884], Corydalis sempervirens [SP|P27793]; contains Pfam chorismate synthase domain PF01264
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA79854.1| Score 78 E Value 3e-25 Identity 64.40% Align. Length 355bp (64.78%) Frame 1
chorismate synthase 2 [Lycopersicon esculentum] pir||S40409 chorismate synthase (EC 4.2.3.5) 2 precursor - tomato sp|Q42885|AROC2_LYCES Chorismate synthase 2, chloroplast precursor (5-enolpyruvylshikimate-3-phosphate phospholyase 2)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA79859.1| Score 77 E Value 6e-27 Identity 73.30% Align. Length 355bp (64.78%) Frame 1
chorismate synthase 1 [Lycopersicon esculentum] pir||S40410 chorismate synthase (EC 4.2.3.5) 1 precursor - tomato sp|Q42884|AROC1_LYCES Chorismate synthase 1, chloroplast precursor (5-enolpyruvylshikimate-3-phosphate phospholyase 1)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0110E09
Clone
Clone name
hba-110e9
LE_HBa0110E09
P110E09
Clone type
BAC
Contamination
none
Estimated length
108,232
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found