Clone Read LE_HBa0110B12_T7_35015

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0110B12_T7_35015
Primer
T7
ID from Seq. Facility
LE_HBa_110_B12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
683 trimmed for vector and quality, 804 raw
Sequence
>LE_HBa0110B12_T7_35015 (raw untrimmed)
ACGCGGGGTCCGAGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGACGTACTGCAACAACTGCCAAGGTATTGTTGTTTACAGAAATTGATCT
CAGATCAGTCAACTGGCCAGGCTTCAGTTTTGGGTACACAGAGTACAGATAAGATGTGATCAAATAATCAAGGACAGCATCTCCAAGAAACTCCAATCTC
TGTTAAATAAAATCAAATGTTCAAAGAGAGAAGTTGCTGTAAGGTTAGTCCTAGCTCGCCTTACGCAACAACGATGAAGGTTTCAGAAAAAAGTAGGTTA
TTTAAGAAGAGCACTTTCATACCTGATAGCAACCCCCTCCATGCCTGTTATAAGATGGATGTATGAATGCCTGAATAAGCAAACCCTTATGGATAAAAGA
ATAACCTAATAAACGTTCAATGCCAAGAACATCTATTTCATCAGCAAGTGGCATAAAGACTTTGCTTGCAGAGCAGATACTTTTTAGTTGTGGTTCTTTG
AAATCTGTATGGATACCAATCCATTTAAGAAATGCAATTGCTGCTTTGAAGCCACTGTCCACTACAAAAGCACCAACAAGGGCTTCAACTATATCAGCAA
TTGTTTTCTTACGCAGCCAGTGATGATATTTGCTACACCTAACTTCAGTCTTTATACCATCTGTTCCGCTACCACACAGACCATGTATGTTTTTTTCTGT
TTGCTTGTTACAAGTTACAGGACCACGACGCCCTACTACATAGAAGTGGTCGGGTTCAAATGATTGATCACCTTTATTGGCCTGAAACAAAAAAAAATGT
AAAA
>LE_HBa0110B12_T7_35015 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTTGACGTACTGCAACAACTGCCAAGGTATTGTTGTTTACAGAAATTGATCTCAGATCAGTCAACTGGCCAG
GCTTCAGTTTTGGGTACACAGAGTACAGATAAGATGTGATCAAATAATCAAGGACAGCATCTCCAAGAAACTCCAATCTCTGTTAAATAAAATCAAATGT
TCAAAGAGAGAAGTTGCTGTAAGGTTAGTCCTAGCTCGCCTTACGCAACAACGATGAAGGTTTCAGAAAAAAGTAGGTTATTTAAGAAGAGCACTTTCAT
ACCTGATAGCAACCCCCTCCATGCCTGTTATAAGATGGATGTATGAATGCCTGAATAAGCAAACCCTTATGGATAAAAGAATAACCTAATAAACGTTCAA
TGCCAAGAACATCTATTTCATCAGCAAGTGGCATAAAGACTTTGCTTGCAGAGCAGATACTTTTTAGTTGTGGTTCTTTGAAATCTGTATGGATACCAAT
CCATTTAAGAAATGCAATTGCTGCTTTGAAGCCACTGTCCACTACAAAAGCACCAACAAGGGCTTCAACTATATCAGCAATTGTTTTCTTACGCAGCCAG
TGATGATATTTGCTACACCTAACTTCAGTCTTTATACCATCTGTTCCGCTACCACACAGACCATGTATGTTTTTTTCTGTTTG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.963328
Expected error %
0.172117
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M19092.1:1-90 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.39%) Frame 2
Cloning vector pJRD179 DNA
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.39%) Frame 2
Cloning vector pMAMneoBlue
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g20320.1 Score 216 E Value 9e-57 Identity 18.40% Align. Length 602bp (88.14%) Frame 1
68418.m02418 DEAD/DEAH box helicase, putative similar to CAF protein [Arabidopsis thaliana] GI:6102610; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain, PF03368: Domain of unknown function, PF00636: RNase3 domain, PF00035: Double-stranded RNA binding motif
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g01040.1 Score 119 E Value 1e-27 Identity 12.50% Align. Length 575bp (84.19%) Frame 1
68414.m00004 DEAD/DEAH box helicase carpel factory / CAF identical to RNA helicase/RNAseIII CAF protein GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_197532.2| Score 216 E Value 4e-55 Identity 18.40% Align. Length 602bp (88.14%) Frame 1
DEAD/DEAH box helicase, putative [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAE03362.1| Score 201 E Value 1e-50 Identity 16.10% Align. Length 602bp (88.14%) Frame 1
OSJNBb0065L13.5 [Oryza sativa (japonica cultivar-group)] ref|XP_473129.1| OSJNBb0065L13.5 [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0110B12
Clone
Clone name
hba-110b12
LE_HBa0110B12
P110B12
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found