Clone Read LE_HBa0106J18_T7_34643

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0106J18_T7_34643
Primer
T7
ID from Seq. Facility
LE_HBa_106_J18-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
606 trimmed for vector and quality, 888 raw
Sequence
>LE_HBa0106J18_T7_34643 (raw untrimmed)
GCTTAACCCGGTCCCGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCAGCTACTTGGTGGCATAGGGAAATAAGTGCATGCATGCATGTTGAC
AAGCTAGAAGACAGAAAAAACTAAATCAGCTTATTCCTAGGCATGAATGTTGAAGCAAAATAGGTTGACTTTACCACTGGAGGCCTTGATAATGCATGTC
CCTCTGGACCAGTAATACCAAGTTCGATCATTCTTTTTTGACCTTCAAATACAGCATCCCCATTTTCTGCATGAACCATAGCTAAGGCACCAAGGGACTT
GCACTTCTTCAATCCCTCCATTAGAAGCTCATCATTAACCATGAAGGAACCTTTGTAGGCAAGGAAAAATTTGAAAGAGTTGATACCTACGATAAGAGAA
ATAAAGTCAAGTGGAGTACCTAAAGAGATACCAAAAAGTGCAGAGAGGAAGATGCTTCCCGTGAACTACTCTGCAAGAATGAACCGAAACTTAAAAAATA
TTCAGATGAAAGTAACACTTTCTGGAAATAAGTAAAACCTTTCTCCTTGACCATAATTTCCATCTCCTCAGATACAGTTTCATTCCATTTTGTAATTGCC
ATGTGAAAACCATAATCCATGCATGACCCTCTAGCTTTCTCTACATAAAGCTTCAAAACCCGCAGAAAATCTTCCATCTACCGGAATAACCAAATCAATA
TGCATTGTTCTTCACCATGCAATTGCGCTGCCTGTCCACTGAAAAACTCAACAATTCTCTCTCATCCCAAAAATTCCATAGCAAAGGTGCTGATGGAGAA
TCAGTCCCCTCGTCTCTACGACAAAATCTAAATTTAAGTAAAATTTTCTGTACTTACAGTTTTTATTTTTGCTTGGCCTCTCAAACCT
>LE_HBa0106J18_T7_34643 (trimmed for quality and cloning vector)
AGCTTCAGCTACTTGGTGGCATAGGGAAATAAGTGCATGCATGCATGTTGACAAGCTAGAAGACAGAAAAAACTAAATCAGCTTATTCCTAGGCATGAAT
GTTGAAGCAAAATAGGTTGACTTTACCACTGGAGGCCTTGATAATGCATGTCCCTCTGGACCAGTAATACCAAGTTCGATCATTCTTTTTTGACCTTCAA
ATACAGCATCCCCATTTTCTGCATGAACCATAGCTAAGGCACCAAGGGACTTGCACTTCTTCAATCCCTCCATTAGAAGCTCATCATTAACCATGAAGGA
ACCTTTGTAGGCAAGGAAAAATTTGAAAGAGTTGATACCTACGATAAGAGAAATAAAGTCAAGTGGAGTACCTAAAGAGATACCAAAAAGTGCAGAGAGG
AAGATGCTTCCCGTGAACTACTCTGCAAGAATGAACCGAAACTTAAAAAATATTCAGATGAAAGTAACACTTTCTGGAAATAAGTAAAACCTTTCTCCTT
GACCATAATTTCCATCTCCTCAGATACAGTTTCATTCCATTTTGTAATTGCCATGTGAAAACCATAATCCATGCATGACCCTCTAGCTTTCTCTACATAA
AGCTTC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.960295
Expected error %
0.458499
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g12200.1 Score 138 E Value 2e-33 Identity 90.40% Align. Length 478bp (78.88%) Frame 2
68418.m01431 dihydropyrimidinase / DHPase / dihydropyrimidine amidohydrolase / hydantoinase (PYD2) identical to dihydropyrimidine amidohydrolase (PYD2) [Arabidopsis thaliana] GI:28194047; similar to SP|Q9EQF5 Dihydropyrimidinase (EC 3.5.2.2) (DHPase) (Hydantoinase) (DHP) {Mus musculus}; contains Pfam profile PF01979: Amidohydrolase family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAB10038.1| Score 138 E Value 9e-32 Identity 30.10% Align. Length 218bp (35.97%) Frame 2
dihydropyrimidinase [Arabidopsis thaliana] gb|AAO33381.1| dihydropyrimidine amidohydrolase [Arabidopsis thaliana] ref|NP_568258.2| dihydropyrimidinase / DHPase / dihydropyrimidine amidohydrolase / hydantoinase (PYD2) [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_915901.1| Score 130 E Value 2e-29 Identity 27.90% Align. Length 218bp (35.97%) Frame 2
putative dihydropyrimidinase [Oryza sativa (japonica cultivar-group)] dbj|BAB44078.1| putative dihydropyrimidine amidohydrolase [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0106J18
Clone
Clone name
hba-106j18
LE_HBa0106J18
C12HBa0106J18
P106J18
Clone type
BAC
Contamination
none
Estimated length
84,181
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found