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Clone Read LE_HBa0100M24_T7_32495

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0100M24_T7_32495
Primer
T7
ID from Seq. Facility
LE_HBa_100_M24-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
742 trimmed for vector and quality, 859 raw
Genbank Accession
DU088069
Sequence
>LE_HBa0100M24_T7_32495 (raw untrimmed)
GCACACCCCGGTACCGGGGGGTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTCTGCAGTGCCCACCAGTGTTCCACTGACCCCCACTGTCCAATTT
AAGAAGAGAATGATGAGCCATGTAGCCAATACATCACAGATCTTGTGAGAATGTGCAAAGCAAAATTAAGGCATACCTGAAATGAGAAGGAGCAGAACTC
CTGAAGAAAACTTGTGTTTTGCTTGGATTGATATGTCTATCAATCCATGATGCCCAAGTTGTCAGGGCTTTTTCGAAAGCTGTTGAAACATCAAGACGAG
GATGTACTTGATTCCTCTCCTGGTAGTAATTCTTCCTGTTGTGACAGTACCAATTATATACGCGAGAGTAACTAGAGGAAGCTAATGCAGCATCCATCAC
ACACAAAAATACTACTAACTTATTTTCTATCTTCATAGTAAGAATTGACAGGATTGATTTTCACCAAAAAACAATCTAGCTCATTAACCGGTATTGCCAG
GTTCTTTCTATATGCTATCACAATATCATGACTAGGTTTCATATCAAAATGTTGGGTACTCCAATTAAACGGCAATTATTTGCCAAAATCTCAAACAAGT
TTAATTTGATCTACATGAATCCTCATTGATGATGTCGCAAAATTTAAGCTCATCTCAGGCCAATATAGGCTTCTCCAATTAAACATCAGTTAAAACCTGC
AAGTAACTTCCAAGTACCATATTCTAAAATAAATCTTTCACATGGATCATTACACTAAAAACTTCTTAAAGAAGAATCTATTCATACCATTAGTCCTTTG
CAGAACTTAAACTCGAAGAAGAACAAATTAACAGAATTACCCTAATAACCTTCAACCAA
>LE_HBa0100M24_T7_32495 (trimmed for quality and cloning vector)
AGCTTCTCTGCAGTGCCCACCAGTGTTCCACTGACCCCCACTGTCCAATTTAAGAAGAGAATGATGAGCCATGTAGCCAATACATCACAGATCTTGTGAG
AATGTGCAAAGCAAAATTAAGGCATACCTGAAATGAGAAGGAGCAGAACTCCTGAAGAAAACTTGTGTTTTGCTTGGATTGATATGTCTATCAATCCATG
ATGCCCAAGTTGTCAGGGCTTTTTCGAAAGCTGTTGAAACATCAAGACGAGGATGTACTTGATTCCTCTCCTGGTAGTAATTCTTCCTGTTGTGACAGTA
CCAATTATATACGCGAGAGTAACTAGAGGAAGCTAATGCAGCATCCATCACACACAAAAATACTACTAACTTATTTTCTATCTTCATAGTAAGAATTGAC
AGGATTGATTTTCACCAAAAAACAATCTAGCTCATTAACCGGTATTGCCAGGTTCTTTCTATATGCTATCACAATATCATGACTAGGTTTCATATCAAAA
TGTTGGGTACTCCAATTAAACGGCAATTATTTGCCAAAATCTCAAACAAGTTTAATTTGATCTACATGAATCCTCATTGATGATGTCGCAAAATTTAAGC
TCATCTCAGGCCAATATAGGCTTCTCCAATTAAACATCAGTTAAAACCTGCAAGTAACTTCCAAGTACCATATTCTAAAATAAATCTTTCACATGGATCA
TTACACTAAAAACTTCTTAAAGAAGAATCTATTCATACCATT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.958809
Expected error %
0.814821
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g62390.1 Score 103 E Value 8e-23 Identity 16.00% Align. Length 305bp (41.11%) Frame 2
68416.m07008 expressed protein several hypothetical proteins - Arabidopsis thaliana
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g12060.1 Score 87 E Value 1e-17 Identity 14.10% Align. Length 296bp (39.89%) Frame 2
68416.m01500 expressed protein similar to hypothetical protein GB:CAB82953 GI:7340710 from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM61621.1| Score 103 E Value 4e-21 Identity 16.00% Align. Length 305bp (41.11%) Frame 2
unknown [Arabidopsis thaliana] emb|CAB82953.1| putative protein [Arabidopsis thaliana] ref|NP_191798.1| expressed protein [Arabidopsis thaliana] pir||T48031 hypothetical protein T12C14.90 - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAB03118.1| Score 87 E Value 4e-16 Identity 14.10% Align. Length 296bp (39.89%) Frame 2
unnamed protein product [Arabidopsis thaliana] gb|AAG51057.1| unknown protein; 38990-36982 [Arabidopsis thaliana] ref|NP_187813.1| expressed protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0100M24
Clone
Clone name
hba-100m24
LE_HBa0100M24
P100M24
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found