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Clone Read LE_HBa0006N20_T7_2967

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0006N20_T7_2967
Primer
T7
ID from Seq. Facility
LE_HBa_006_N20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
858 trimmed for vector and quality, 964 raw
Genbank Accession
DU023128
Sequence
>LE_HBa0006N20_T7_2967 (raw untrimmed)
GGGCCCTACTGGTACCGGGGGNTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTATCGTACGAGATCCTCAAGCCTTTAATGATTTTTACAAAGAAA
AAACTAAGATTAGGAATGACATTGAAGCTGTAAAACATGAGAGGCATGCAAGATCTGATCTGCCAAGGCAAGAATCCCATCGTAACTCTCGTGAGCATAC
AAGGTCTGATATACCAAGGCATGAATCCCATCGTAACTCTCGTGAGCATACAAGGTCTGATATACCAAGGCATGAATCCCATCGTAACTCTCATGAGCAG
ACAAGATCTGATATACCAAGGGACGAATCCCATCGTAACTCTCGTGAGCAGACAAGATCTGATCCTCCAAGGCACGAGTCGCGTCATAACTCTCGCAAGC
CTGTTAGGCTCTCTGAAGAAGAGAGAGCTGCCCGTCTACGGGAGATGCAGAAAGATGCTGAGGTCCATGAGGAGCAAAGATGGAAAAGACTCAAGAAGGC
AGATGAAAATGATGCTAAGGAAGCTGTACATGCTGGCTCGTCTGGTGGTAGGAATTTTCTGGATGCTGCTCAAAGAAGTGTTTATGGTGCTGGAAAGGGA
GGAAGCTCCACAATTGAGGAAAGTGTTCGTCGCCGAACACACTATTCACAGAGAGCAGAAGCTTCAGAAGGCAATGCTTTTCGGCGATAAAATGGATGAA
AAAATTAATGAAGTTCCTGCTGAGTTTTTCCATAATGAATCACTTTATGTAATCCAAATGTATTTCATTTTGATCACACTGTTCTATTATCATACTGAGC
GATTATTGTGGGATCAAGGTCAGATTAAGTATGCTGGTGTTCATCTTGCCACAAATATACTTGGTGTAACTGAAATCGTGAACTTGTTATGTGCAAAAAA
AATGTTTTTAAGGTGGCTGGCTTATTACTTGAGCTGTGAGTTTGTGCTGTTAGCCTTTGATCCG
>LE_HBa0006N20_T7_2967 (trimmed for quality and cloning vector)
AGCTTCTATCGTACGAGATCCTCAAGCCTTTAATGATTTTTACAAAGAAAAAACTAAGATTAGGAATGACATTGAAGCTGTAAAACATGAGAGGCATGCA
AGATCTGATCTGCCAAGGCAAGAATCCCATCGTAACTCTCGTGAGCATACAAGGTCTGATATACCAAGGCATGAATCCCATCGTAACTCTCGTGAGCATA
CAAGGTCTGATATACCAAGGCATGAATCCCATCGTAACTCTCATGAGCAGACAAGATCTGATATACCAAGGGACGAATCCCATCGTAACTCTCGTGAGCA
GACAAGATCTGATCCTCCAAGGCACGAGTCGCGTCATAACTCTCGCAAGCCTGTTAGGCTCTCTGAAGAAGAGAGAGCTGCCCGTCTACGGGAGATGCAG
AAAGATGCTGAGGTCCATGAGGAGCAAAGATGGAAAAGACTCAAGAAGGCAGATGAAAATGATGCTAAGGAAGCTGTACATGCTGGCTCGTCTGGTGGTA
GGAATTTTCTGGATGCTGCTCAAAGAAGTGTTTATGGTGCTGGAAAGGGAGGAAGCTCCACAATTGAGGAAAGTGTTCGTCGCCGAACACACTATTCACA
GAGAGCAGAAGCTTCAGAAGGCAATGCTTTTCGGCGATAAAATGGATGAAAAAATTAATGAAGTTCCTGCTGAGTTTTTCCATAATGAATCACTTTATGT
AATCCAAATGTATTTCATTTTGATCACACTGTTCTATTATCATACTGAGCGATTATTGTGGGATCAAGGTCAGATTAAGTATGCTGGTGTTCATCTTGCC
ACAAATATACTTGGTGTAACTGAAATCGTGAACTTGTTATGTGCAAAAAAAATGTTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.975373
Expected error %
0.686833
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g44200.1 Score 132 E Value 3e-31 Identity 14.90% Align. Length 605bp (70.51%) Frame 3
68415.m05500 expressed protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM47345.1| Score 132 E Value 2e-29 Identity 14.90% Align. Length 605bp (70.51%) Frame 3
At2g44200/F6E13.34 [Arabidopsis thaliana] gb|AAC16071.1| expressed protein [Arabidopsis thaliana] gb|AAM14905.1| expressed protein [Arabidopsis thaliana] gb|AAK74011.1| At2g44200/F6E13.34 [Arabidopsis thaliana] pir||T02376 hypothetical protein At2g44200 [imported] - Arabidopsis thaliana ref|NP_566014.1| expressed protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_483186.1| Score 117 E Value 3e-25 Identity 13.40% Align. Length 590bp (68.76%) Frame 3
unknown protein [Oryza sativa (japonica cultivar-group)] dbj|BAD08813.1| unknown protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0006N20
Clone
Clone name
hba-6n20
LE_HBa0006N20
P006N20
Clone type
BAC
Contamination
none
Estimated length
108,868
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found