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Clone Read LE_HBa0095F11_T7_28293

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0095F11_T7_28293
Primer
T7
ID from Seq. Facility
LE_HBa_095_F11-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
678 trimmed for vector and quality, 939 raw
Sequence
>LE_HBa0095F11_T7_28293 (raw untrimmed)
CCCGGGGGGGGATCCTCCTAGAGTCGACCTGCAGGCATGCAAGCTTGTATACTAATTGTTTTTTTAAAAGGCATGTCATTTTCCTATTGAGTTTGAGCAG
AGAGCACTATGGGCATTGAAGAAGCTGAATCTCAACTTTAGTGAAACAGTGAACTTGAGGCTAGATCAGATTAACGTGATGGATACATTGATTAAAAGAG
CTTATGATATAACATCATTGTATAAGGAGAGGATTAATCTCTATCATGATAAACAAATCTAAAAGAATCTTACCCTTGGAGACTTCGTTCTACAATTAAA
CTCCATAATTTGTCTATTTCTTGATAAACTGAGATCTAAATGGTCTGGAACTTTTAAGTTTGCTTAAGTATTTCAATCTAGTGATGTTTAGTTGGAAAAT
GAGAAAAGTGGGAGATTTAAAGTAAATTATCAGAAAAATCAAGGCATATTTGGGAGTCTAGATGAGGCTAATATTGTAGATAATTGCAAACATGATGAAG
TCTGAGTAATCAAGTAAACTGATTCGCGGCACAACATTAAATAATGCCCTACTTGGGAGGCAACCCAAGGTGTTATTAAGTTAGGTATTTTAATGTGTTG
TGTTTTTTTTTAATTTTGGTTCATAGAGTTATAGGGTGCTGATATGTGCATAAAGGATTAAGTGAAAAAAATTCAGAAAAATTGGTACAACTTGCACCTA
ACAGTATAACTCATGGACTGTTGGTGCACTTCATGGGCACTTTCTCAGTCATCATCTCTAATTATAAGAAGGAGGCTGCTTTTAAACTTTTTCCGATTAA
TTTAGTTTTTGTGTACCTTCGGATGAAATACATAATTTCTTGTTTATTCCCCCGCCCTCCTTTGGAACATCAGCCTCTTCGAGATACCTCTCGCTCCTTA
CCTCCCTTCTCCATGGCTTTCCATACTCTTTTCTCTCTA
>LE_HBa0095F11_T7_28293 (trimmed for quality and cloning vector)
CCTAGAGTCGACCTGCAGGCATGCAAGCTTGTATACTAATTGTTTTTTTAAAAGGCATGTCATTTTCCTATTGAGTTTGAGCAGAGAGCACTATGGGCAT
TGAAGAAGCTGAATCTCAACTTTAGTGAAACAGTGAACTTGAGGCTAGATCAGATTAACGTGATGGATACATTGATTAAAAGAGCTTATGATATAACATC
ATTGTATAAGGAGAGGATTAATCTCTATCATGATAAACAAATCTAAAAGAATCTTACCCTTGGAGACTTCGTTCTACAATTAAACTCCATAATTTGTCTA
TTTCTTGATAAACTGAGATCTAAATGGTCTGGAACTTTTAAGTTTGCTTAAGTATTTCAATCTAGTGATGTTTAGTTGGAAAATGAGAAAAGTGGGAGAT
TTAAAGTAAATTATCAGAAAAATCAAGGCATATTTGGGAGTCTAGATGAGGCTAATATTGTAGATAATTGCAAACATGATGAAGTCTGAGTAATCAAGTA
AACTGATTCGCGGCACAACATTAAATAATGCCCTACTTGGGAGGCAACCCAAGGTGTTATTAAGTTAGGTATTTTAATGTGTTGTGTTTTTTTTTAATTT
TGGTTCATAGAGTTATAGGGTGCTGATATGTGCATAAAGGATTAAGTGAAAAAAATTCAGAAAAATTGGTACAACTTG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.942525
Expected error %
0.10801
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65302.1:2832-2867-49 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.72%) Frame 3
Cloning vector pGEM-3
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65302.1:1-99 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.72%) Frame 3
Cloning vector pGEM-3
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAQ82031.1| Score 97 E Value 4e-19 Identity 13.40% Align. Length 380bp (56.05%) Frame 2
pol [Pisum sativum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM94940.1| Score 96 E Value 8e-19 Identity 14.20% Align. Length 380bp (56.05%) Frame 2
conserved hypothetical protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0095F11
Clone
Clone name
hba-95f11
LE_HBa0095F11
C12HBa0095F11
P095F11
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found