Clone Read LE_HBa0177B17_SP6_263979

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0177B17_SP6_263979
Primer
SP6
ID from Seq. Facility
LE_HBa_177_B17-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
454 trimmed for vector and quality, 914 raw
Sequence
>LE_HBa0177B17_SP6_263979 (raw untrimmed)
GAAATCCAATTGGCTTAGAAGCATGTCTATCGAACAGGTCAAATGATGAAATTCTTCTCGTCAAAAAGTTGCTATGTTATGACCCGGCTGGTAGAGCTAC
TGCAATGGAATTACTCCACGACAAGTATCTAAACGAAGATCCCTTGCCAGTCCCATCGTCTGAATTGAGGATTCCCTCCAAACACAGCTCTGAAAATGAT
GATTCACCTGAAGAATTTCATAGAGATCTGAACTCCGACTCAGACTTTGATGATTTTGGCCCAACGAAGGTTTCAACCACGGATCATGGTTTCTCAATTC
AGTTTTCGTGAGCTAGGAAGTCCGTTTTTATTTTTCAGAATTTGTTCTCCAGACTACCGCGTGATGATCAACATCATCTTTTGCTGCAGACTATTTAAAC
TATGCAGCACTTCATATATTTTGTTGGAACAGATTCTCAAGTTTTTGCAAGTTGTAACTATTATGGCGTCCCTTATTGGAATACCCAAATAGAGAAATCT
ATGACCTTTTTTACACTTTTCCAACATATTTTATCATTGGGATTTCTTCTCATCAGACCTTTACCATGAGTTACTATTTGTTATCTACTGTCTCGCTGGA
AACTTCCAGGTATTTCGTTGATACTAGTTACTTCATGCTTCTTCGAACTCCCGTGATTATCTTTTTTACTTTTATTTCATATCACCGTTAAATGCACTCA
TCTCTGGTTTCTTTTCTAGTACCATTTCTACTTCTGTATCCCCATGATCTTCATGTCATTCACTTTTGCTTTATTTATTCTTGGCGAAGTTTCATATATC
ATTCATTTTATCCTCATGTCTTCATTGCTGTATATTTCGTTCTATCTCTATGGTTTAGATCCGGTTCTGTTATATCTTTTGTTTCCTCCCCTAGTAACTC
TTCTTCTCTTCTCG
>LE_HBa0177B17_SP6_263979 (trimmed for quality and cloning vector)
AATCCAATTGGCTTAGAAGCATGTCTATCGAACAGGTCAAATGATGAAATTCTTCTCGTCAAAAAGTTGCTATGTTATGACCCGGCTGGTAGAGCTACTG
CAATGGAATTACTCCACGACAAGTATCTAAACGAAGATCCCTTGCCAGTCCCATCGTCTGAATTGAGGATTCCCTCCAAACACAGCTCTGAAAATGATGA
TTCACCTGAAGAATTTCATAGAGATCTGAACTCCGACTCAGACTTTGATGATTTTGGCCCAACGAAGGTTTCAACCACGGATCATGGTTTCTCAATTCAG
TTTTCGTGAGCTAGGAAGTCCGTTTTTATTTTTCAGAATTTGTTCTCCAGACTACCGCGTGATGATCAACATCATCTTTTGCTGCAGACTATTTAAACTA
TGCAGCACTTCATATATTTTGTTGGAACAGATTCTCAAGTTTTTGCAAGTTGTA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.965777
Expected error %
0.207663
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g28980.2 Score 125 E Value 8e-30 Identity 19.10% Align. Length 302bp (66.52%) Frame 2
68417.m04140 cyclin-dependent kinase-activating kinase 1At / CDK-activating kinase 1At (CAK1) identical to Cdk-activating kinase 1At [Arabidopsis thaliana] gi|3218550|dbj|BAA28775
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g28980.1 Score 125 E Value 8e-30 Identity 19.10% Align. Length 302bp (66.52%) Frame 2
68417.m04139 cyclin-dependent kinase-activating kinase 1At / CDK-activating kinase 1At (CAK1) identical to Cdk-activating kinase 1At [Arabidopsis thaliana] gi|3218550|dbj|BAA28775
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB79656.1| Score 125 E Value 3e-28 Identity 19.10% Align. Length 302bp (66.52%) Frame 2
Cdk-activating kinase CAK1At [Arabidopsis thaliana] emb|CAB43912.1| Cdk-activating kinase CAK1At [Arabidopsis thaliana] dbj|BAA28775.1| Cdk-activating kinase 1At [Arabidopsis thaliana] gb|AAL47340.1| Cdk-activating kinase CAK1At [Arabidopsis thaliana] ref|NP_194627.1| cyclin-dependent kinase-activating kinase 1At / CDK-activating kinase 1At (CAK1) [Arabidopsis thaliana] ref|NP_849468.1| cyclin-dependent kinase-activating kinase 1At / CDK-activating kinase 1At (CAK1) [Arabidopsis thaliana] gb|AAK68756.1| Cdk-activating kinase CAK1At [Arabidopsis thaliana] pir||T08953 Cdk-activating protein kinase (EC 2.7.1.-) 1 [validated] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD61885.1| Score 112 E Value 4e-24 Identity 18.80% Align. Length 302bp (66.52%) Frame 2
putative cyclin-dependent kinase-activating kinase 1At [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0177B17
Clone
Clone name
hba-177b17
LE_HBa0177B17
P177B17
Clone type
BAC
Contamination
none
Estimated length
85,964
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found