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Clone Read LE_HBa0107L11_SP6_258714

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0107L11_SP6_258714
Primer
SP6
ID from Seq. Facility
LE_HBa_107_L11-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
598 trimmed for vector and quality, 885 raw
Genbank Accession
DU082229
Sequence
>LE_HBa0107L11_SP6_258714 (raw untrimmed)
TTTCCAGTGAGAGATTTTAGACCCTCAGGCTTCCTGCTCCGTTCTTCCTGCAGCACAGCGACCAAAACACAGCATTAACACACATGTGAAGAACGGAGAG
CAGGCACAAAAATGGATTACCTTAGACCACTCAACACGAAGCCTCCGTCCCTTTCTGCCAAATTCTATTCGGTCAAGCCCCTGAATAGCATCCTTTGCAT
CTCTTTCATCATCCATATAAACAAAAGCAAAACCTGAAGTTTGATGCAAGAAATAAACTGAAGATTAAACAAAAACTCAGTTGGTGACAACAAGACCCCC
CCAAAGAAGGATTCCACTAATCATAAATGCTCAACAAAAAAAAAAAAAAGAAAGATAACTTTATCCAGTAAATGTGCAAACCATCTAGCTAGCATTTCAT
TGCATTACAATCACTCCTGTCAAATCGACAAACTTTGACACCATCAAAATCAGCAACAGACCTTCAATGGTGGATCTAAAGATGCCAGGGAAAGGTGGAT
GTCACATCATGGGATAATGTTAAAGAGTGCCTTCTCCATTTTAAACTGCAGCCTCAGCTTCGAGGTCGGAATTGTGACAAGTCTGAAAATGAAGGTGGGG
CAAAATTCACGAGGAATGGAGGGCAATAGTGACTGCACACNNTNTGTNNTTCCCATGCCATGATGGATCAGAGAGCTCTTGTTGAAACAAGAAGAGCAAC
TTGTCACCGGTCAGGACATGAAATAGACACCCTCCACCAGCAATGGGTATGGACATTGCAATGTCATCCTGCGCCCAAAAATCAGTTGAATATGGGGGTC
TACCCTTTAAGAAAATTTACTTTTTTCAGGTAAACATATCCTACTACCCCCACCAAACTCTACCAAAATTTCTGGCTGTTGAGCA
>LE_HBa0107L11_SP6_258714 (trimmed for quality and cloning vector)
CTCAGGCTTCCTGCTCCGTTCTTCCTGCAGCACAGCGACCAAAACACAGCATTAACACACATGTGAAGAACGGAGAGCAGGCACAAAAATGGATTACCTT
AGACCACTCAACACGAAGCCTCCGTCCCTTTCTGCCAAATTCTATTCGGTCAAGCCCCTGAATAGCATCCTTTGCATCTCTTTCATCATCCATATAAACA
AAAGCAAAACCTGAAGTTTGATGCAAGAAATAAACTGAAGATTAAACAAAAACTCAGTTGGTGACAACAAGACCCCCCCAAAGAAGGATTCCACTAATCA
TAAATGCTCAACAAAAAAAAAAAAAAGAAAGATAACTTTATCCAGTAAATGTGCAAACCATCTAGCTAGCATTTCATTGCATTACAATCACTCCTGTCAA
ATCGACAAACTTTGACACCATCAAAATCAGCAACAGACCTTCAATGGTGGATCTAAAGATGCCAGGGAAAGGTGGATGTCACATCATGGGATAATGTTAA
AGAGTGCCTTCTCCATTTTAAACTGCAGCCTCAGCTTCGAGGTCGGAATTGTGACAAGTCTGAAAATGAAGGTGGGGCAAAATTCACGAGGAATGGAG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.964517
Expected error %
0.149915
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g25500.1 Score 72 E Value 2e-13 Identity 27.40% Align. Length 116bp (19.40%) Frame 3
68417.m03673 arginine/serine-rich splicing factor RSP40 (RSP40) identical to SP|P92965 Arginine/serine-rich splicing factor RSP40 {Arabidopsis thaliana}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g52040.2 Score 69 E Value 2e-12 Identity 25.60% Align. Length 116bp (19.40%) Frame 3
68418.m06459 arginine/serine-rich splicing factor RSP41 (RSP41) nearly identical to SP|P92966 Arginine/serine-rich splicing factor RSP41 {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA67800.1| Score 72 E Value 7e-12 Identity 27.40% Align. Length 116bp (19.40%) Frame 3
splicing factor [Arabidopsis thaliana] emb|CAA18176.1| splicing factor At-SRp40 [Arabidopsis thaliana] ref|NP_194280.1| arginine/serine-rich splicing factor RSP40 (RSP40) [Arabidopsis thaliana] sp|P92965|RSP40_ARATH Arginine/serine-rich splicing factor RSP40
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB81360.1| Score 72 E Value 7e-12 Identity 27.40% Align. Length 116bp (19.40%) Frame 3
splicing factor At-SRp40 [Arabidopsis thaliana] pir||F85294 splicing factor At-SRp40 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0107L11
Clone
Clone name
hba-107l11
LE_HBa0107L11
P107L11
Clone type
BAC
Contamination
none
Estimated length
132,685
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found