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Clone Read LE_HBa0045K04_SP6_258319

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0045K04_SP6_258319
Primer
SP6
ID from Seq. Facility
LE_HBa_045_K04-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
737 trimmed for vector and quality, 866 raw
Genbank Accession
DU081280
Sequence
>LE_HBa0045K04_SP6_258319 (raw untrimmed)
NNNCCGCTCCCAGAGGCATATGGACCTTCGTCAGACAAAAATATTTTTCTTTTATCAGGAGTTACATTGACATCAAATTCTCTTGGTGGCATCGCAAAAT
TCATGATTGCAATGGGATATTGCCGAGAGTTTGCACCTCTATATAATTCATTGATAAGCTTGCCAACTTTTGGCATATCCACCGGTCGTCCATTCACAAA
AAAATATTGTCGATCTCCTAAGTTGCGTCCACTACCATAGCCAGACTTGGAAATGAATCCTTCAACAGTGCAATCATCAGACATACACACTTCCAGAGGC
TCCAGACAAGTGAAGGTACTCATACCAAATACTGTTATAATGTTATCTTTCAGAGATCCACTTCCCTGAGTCTTCAGAACTACAGATTTTGCATTTTTTA
GAGCTGAGTTGGTACAAACTAGTCTGACTCCCTTAGAAATAAGAGCATAGGCCTAGAAGTTCAACCAACAAAAGAAAGCAAATAAATAACTTAAACAACA
AAAGCATTAAAATACTTCTTCAGCAGAAACCATGCACATACTCTCTCAAGATTAAGAACCTTGACAAGAACAAATTATTAAGCAGTCTAACATGAAAGGT
CACCAAACTATAGCACAATGAAATTCACAATCACCAGAAGCAAAAAGATTTTTTGATGAAGTTAATCGATTTCACTAAAGAGAGAAATCTCCCCTATACA
AGAAGTATACCCAAAGAAAAAGTGGATAGCCTACACAAAAGTATGAGTCTCTACATATTGCTCCACAATTTGTGCAATCCTTCATTTAGATAGGAAACTT
CATTTCCTGGGAAGCTTTTGTATTTCCTTACCCTTGGTATGCTTGAGTTCATAAAGGGGCTCAAAA
>LE_HBa0045K04_SP6_258319 (trimmed for quality and cloning vector)
CATATGGACCTTCGTCAGACAAAAATATTTTTCTTTTATCAGGAGTTACATTGACATCAAATTCTCTTGGTGGCATCGCAAAATTCATGATTGCAATGGG
ATATTGCCGAGAGTTTGCACCTCTATATAATTCATTGATAAGCTTGCCAACTTTTGGCATATCCACCGGTCGTCCATTCACAAAAAAATATTGTCGATCT
CCTAAGTTGCGTCCACTACCATAGCCAGACTTGGAAATGAATCCTTCAACAGTGCAATCATCAGACATACACACTTCCAGAGGCTCCAGACAAGTGAAGG
TACTCATACCAAATACTGTTATAATGTTATCTTTCAGAGATCCACTTCCCTGAGTCTTCAGAACTACAGATTTTGCATTTTTTAGAGCTGAGTTGGTACA
AACTAGTCTGACTCCCTTAGAAATAAGAGCATAGGCCTAGAAGTTCAACCAACAAAAGAAAGCAAATAAATAACTTAAACAACAAAAGCATTAAAATACT
TCTTCAGCAGAAACCATGCACATACTCTCTCAAGATTAAGAACCTTGACAAGAACAAATTATTAAGCAGTCTAACATGAAAGGTCACCAAACTATAGCAC
AATGAAATTCACAATCACCAGAAGCAAAAAGATTTTTTGATGAAGTTAATCGATTTCACTAAAGAGAGAAATCTCCCCTATACAAGAAGTATACCCAAAG
AAAAAGTGGATAGCCTACACAAAAGTATGAGTCTCTA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.952116
Expected error %
0.161799
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g02460.1 Score 213 E Value 1e-55 Identity 23.00% Align. Length 425bp (57.67%) Frame 3
68417.m00333 DNA mismatch repair protein, putative similar to SP|P54278 PMS1 protein homolog 2 (DNA mismatch repair protein PMS2) {Homo sapiens}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF01119: DNA mismatch repair protein, C-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_466491.1| Score 213 E Value 3e-54 Identity 23.00% Align. Length 425bp (57.67%) Frame 3
putative PMS2 postmeiotic segregation increased 2 [Oryza sativa (japonica cultivar-group)] dbj|BAD34084.1| putative PMS2 postmeiotic segregation increased 2 [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL01156.1| Score 213 E Value 5e-54 Identity 23.00% Align. Length 425bp (57.67%) Frame 3
DNA mismatch repair protein [Arabidopsis thaliana] ref|NP_567236.1| DNA mismatch repair protein, putative [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0045K04
Clone
Clone name
hba-45k4
LE_HBa0045K04
P045K04
Clone type
BAC
Contamination
none
Estimated length
115,020
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found