Clone Read LE_HBa0089E04_T7_25159

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0089E04_T7_25159
Primer
T7
ID from Seq. Facility
LE_HBa_089_E04-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
776 trimmed for vector and quality, 926 raw
Genbank Accession
DU039996
Sequence
>LE_HBa0089E04_T7_25159 (raw untrimmed)
CTAGACTGGAACCCGGGGTTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAAAATCAGTAGTAGAATTGCTCATTAATTAAAGTGAGACTGCCTAATG
CAACTAATTTTTTTCCTCAGAGCTGATTAATTGAACAAAATACAAAGGTTTGAACGGTAAATCCCAATCTCCAAAAGTTTAGAAGGGTCCAGATCACTAG
CTTCGAAAAATTTTAGGTCATGGTCACTAGTATGACCCTTTATTAACTGGAGTGCAATTTTAGAACAAAGAACTATTCTAAAATTTTACTTCACAAGAAT
GTCTGCAACTTACCCAATGCACAAACAGCAATGGCCAATTAAATGAGCACAGGGAAGATGTGTTAGGATTCAAGAAATGGTATTGAAGCAATAGTTCAAT
TTGGTGTTTCAATATTTTAATGTAATGCAAAACCAAAACTGTAGAAAATGGCAGATGTTAACTTCTACCCAAATAGCCAATTATGGATATTGATGATTTC
CAAAAACAAAAGTAACACACACCTGACTGCTACTGTTAACCATGTAGCTTAGAAGCAAATGCACATCCAATGCAGATTCAAACATCACTTTAGCAACCTG
AGAAGTCGCCTGCACTGTAAGGGCAGTGCTGACTGTGAAGAGTCCTTCAGAATCCAAGTTATGCTCATATTTTCGAGGCACGAGAAATTTCAAATAGCTT
TCAATAACATGCAAAACGCTCCTCCATGTGGTAGCTCTACTGCACAAGTTGTGCAGGGATAAAAACATATCTACTGAAAATTTTTCTGAGACTTTTGTGG
TAGGAAATTTCCTTATTCAATGCTACATCAGTTCCAAGTTCGGGATAAATGGAATGCCATTGATGAACTATATCCACTATCTAAATTTTTCCGCAACCGA
GGGATTACTTCCTTCAAAGATAAATT
>LE_HBa0089E04_T7_25159 (trimmed for quality and cloning vector)
AGCTTAAAATCAGTAGTAGAATTGCTCATTAATTAAAGTGAGACTGCCTAATGCAACTAATTTTTTTCCTCAGAGCTGATTAATTGAACAAAATACAAAG
GTTTGAACGGTAAATCCCAATCTCCAAAAGTTTAGAAGGGTCCAGATCACTAGCTTCGAAAAATTTTAGGTCATGGTCACTAGTATGACCCTTTATTAAC
TGGAGTGCAATTTTAGAACAAAGAACTATTCTAAAATTTTACTTCACAAGAATGTCTGCAACTTACCCAATGCACAAACAGCAATGGCCAATTAAATGAG
CACAGGGAAGATGTGTTAGGATTCAAGAAATGGTATTGAAGCAATAGTTCAATTTGGTGTTTCAATATTTTAATGTAATGCAAAACCAAAACTGTAGAAA
ATGGCAGATGTTAACTTCTACCCAAATAGCCAATTATGGATATTGATGATTTCCAAAAACAAAAGTAACACACACCTGACTGCTACTGTTAACCATGTAG
CTTAGAAGCAAATGCACATCCAATGCAGATTCAAACATCACTTTAGCAACCTGAGAAGTCGCCTGCACTGTAAGGGCAGTGCTGACTGTGAAGAGTCCTT
CAGAATCCAAGTTATGCTCATATTTTCGAGGCACGAGAAATTTCAAATAGCTTTCAATAACATGCAAAACGCTCCTCCATGTGGTAGCTCTACTGCACAA
GTTGTGCAGGGATAAAAACATATCTACTGAAAATTTTTCTGAGACTTTTGTGGTAGGAAATTTCCTTATTCAATGC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.951547
Expected error %
0.53748
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g33410.1 Score 92 E Value 3e-19 Identity 16.90% Align. Length 260bp (33.51%) Frame 3
68414.m04136 expressed protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_174607.1| Score 92 E Value 2e-17 Identity 16.90% Align. Length 260bp (33.51%) Frame 3
expressed protein [Arabidopsis thaliana] gb|AAG51224.1| unknown protein; 42586-33527 [Arabidopsis thaliana] pir||G86457 unknown protein, 42586-33527 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_464115.1| Score 79 E Value 2e-13 Identity 14.10% Align. Length 290bp (37.37%) Frame 3
Nucleoporin-like protein [Oryza sativa (japonica cultivar-group)] dbj|BAD10644.1| Nucleoporin-like protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0089E04
Clone
Clone name
hba-89e4
LE_HBa0089E04
P089E04
Clone type
BAC
Contamination
none
Estimated length
125,696
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found