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Clone Read LE_HBa0005L01_SP6_2064

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0005L01_SP6_2064
Primer
SP6
ID from Seq. Facility
LE_HBa_005_L01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
712 trimmed for vector and quality, 863 raw
Genbank Accession
DU022311
Sequence
>LE_HBa0005L01_SP6_2064 (raw untrimmed)
AAAGGGGCTNCCGCCCAGCTCTGTANTACACCTGGATTAGTCCAAGAAGCTAGCATTATCTAGTGATAAATTAATACACTCAGTTTTAATTTCAAAAAAA
TACTAAAGAGGATAATTCATATTTGAATTCAGTGACAGGATTAAAATGCATAAACTTAATACCTTTCAGCACTAAAAGTTCAGTAAAGTAATTCAATGGT
ACAATTTCAAAAAGAATTCAGCCTTCAGCTTTCTCAAATAGCTCTCAGTCAACCATTAAATGAAAAATGTACATCATAACATATGAAACCATCAAGATCT
TACACCGTGACAGAGAAAACTCACTTAAAATCATATCCAGTTCCAGGCTGCAGAACTTCTGGTGCCATCCTACAAAATAAAAGTAGTTCATGCCTTGATA
GACATGTAGAGCACGAAAAAACTTGCATAGAAACAATTATAGATAGGAAGTGACTGAAAGGATGCCTATATGCTCACCAGCACGGAGTTCCTACAAAGGT
ATTCCTAGACCGCTGTCTATCACCACTGTCAAACATGCATGCGGAAACACCAAAATCGCCCAGCTTTACTGCCCCATTAGTGTCCAGTAATATATTCCCA
GCCTGGGATGAGAAAAGTGGTAGAGAAACCACAAATATCAGAATTTGAGGGTCAAAAACAAGGGAACATCGGTTTTAACAAACCTTCACATCTCGATGTA
TGTGCCCATGTCGATGGAGATATTCCAACGCCTTGAGAGTTTCCTTCAACATAGAACAAATAGCAGATTTCTCGAATCCATCTGGATATGCTATTTTCAT
GAGATGTAGACAAGAGCCCTCAGCCATGAAGGGCATTTACCACCCATTGGGTAGTTTTCCACA
>LE_HBa0005L01_SP6_2064 (trimmed for quality and cloning vector)
AGTCCAAGAAGCTAGCATTATCTAGTGATAAATTAATACACTCAGTTTTAATTTCAAAAAAATACTAAAGAGGATAATTCATATTTGAATTCAGTGACAG
GATTAAAATGCATAAACTTAATACCTTTCAGCACTAAAAGTTCAGTAAAGTAATTCAATGGTACAATTTCAAAAAGAATTCAGCCTTCAGCTTTCTCAAA
TAGCTCTCAGTCAACCATTAAATGAAAAATGTACATCATAACATATGAAACCATCAAGATCTTACACCGTGACAGAGAAAACTCACTTAAAATCATATCC
AGTTCCAGGCTGCAGAACTTCTGGTGCCATCCTACAAAATAAAAGTAGTTCATGCCTTGATAGACATGTAGAGCACGAAAAAACTTGCATAGAAACAATT
ATAGATAGGAAGTGACTGAAAGGATGCCTATATGCTCACCAGCACGGAGTTCCTACAAAGGTATTCCTAGACCGCTGTCTATCACCACTGTCAAACATGC
ATGCGGAAACACCAAAATCGCCCAGCTTTACTGCCCCATTAGTGTCCAGTAATATATTCCCAGCCTGGGATGAGAAAAGTGGTAGAGAAACCACAAATAT
CAGAATTTGAGGGTCAAAAACAAGGGAACATCGGTTTTAACAAACCTTCACATCTCGATGTATGTGCCCATGTCGATGGAGATATTCCAACGCCTTGAGA
GTTTCCTTCAAC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.95777
Expected error %
0.0279485
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g24100.1 Score 118 E Value 4e-27 Identity 16.00% Align. Length 425bp (59.69%) Frame 2
68417.m03460 protein kinase family protein contains eukaryotic protein kinase domain, INTERPRO:IPR000719
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g10730.1 Score 116 E Value 1e-26 Identity 15.70% Align. Length 425bp (59.69%) Frame 2
68417.m01753 protein kinase family protein contains protein kinase domain, Pfam:PF00069
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_912431.1| Score 122 E Value 9e-27 Identity 17.10% Align. Length 425bp (59.69%) Frame 2
Putative Ste20-related protein kinase [Oryza sativa (japonica cultivar-group)] gb|AAO17022.1| Putative Ste20-related protein kinase [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC35524.1| Score 121 E Value 2e-26 Identity 16.20% Align. Length 425bp (59.69%) Frame 2
contains similarity to protein kinase domains (Pfam: pkinase.hmm, score: 227.04) [Arabidopsis thaliana] pir||T01904 hypothetical protein T12H20.4 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0005L01
Clone
Clone name
hba-5l1
LE_HBa0005L01
C08HBa0005L01
P005L01
Clone type
BAC
Contamination
none
Estimated length
122,841
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found