Clone Read LE_HBa0079P08_T7_20319

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0079P08_T7_20319
Primer
T7
ID from Seq. Facility
LE_HBa_079_P08-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
683 trimmed for vector and quality, 877 raw
Genbank Accession
DU037454
Sequence
>LE_HBa0079P08_T7_20319 (raw untrimmed)
CCCTAACCTGGTATAGNNGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGAACAACTTGTGAGCTGATGTATCAAGAAGTTTTTCAGCCACC
GGATTAAATAAGATAAAAGTAGTGTCTCCAGTCCTATCTGTCACTTTTAGGTGTATCTTGTACCTATGTTTACATAATAAGTTAGATTCATTCACAACAT
ATAAAAACCTAATTCACAGTCCTTCGTAAATGGTTACCTGACCAAAGGAAATTTGCAATCTTTGTTGAAATTGTGGCATCGATAAATACTATTTGTTGGT
TCAATCTTCTTTGAGCACACATTGCATGAAATGTAGTACCAACCAAAATAGTTATGTATTTCTATTATCTTGCTCTTCACTGTAACAATGTATTCCTACA
ATTTTAATTAAATATCATGAAATAGTGTAATGATCATATTTAAATGGTAAACCAATTATGTGAGAAGCGTAATAAGAAAAGAACCTGTAGTTCAGAGCTC
CACTCAGCCTCCAGCAACTCCTTAATGTCCATACGGTTCAAAAACATCTCTTCCTCACGAGGAAGACTGTTAACATTAGAGCTTTTTATAATTTGAACTT
CATTGGACATGGTGGAAAATTTTGGAGCCAAAGATCTTATGTAATCTATATCAAGATTCACATATATTTTGCTTGCATAGGTGGTGGAGAAGCTAACCTC
ACCTATTGGGTTAAAAATGAAAGCATGTCATTTCTTTAAAAACGCTTGGAGTTAAATTGCTAGTAAGTACAACTAATCTATAAACAATACCACGAAATTC
CTCCCTGTTGGAGAATCCACTAACCTTTATATGGGCCAAATCATTGTTAACAAATAAGGACAAAATTTTCCCCAATT
>LE_HBa0079P08_T7_20319 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGAACAACTTGTGAGCTGATGTATCAAGAAGTTTTTCAGCCACCGGATTAAATAAGATAAAAGTAGT
GTCTCCAGTCCTATCTGTCACTTTTAGGTGTATCTTGTACCTATGTTTACATAATAAGTTAGATTCATTCACAACATATAAAAACCTAATTCACAGTCCT
TCGTAAATGGTTACCTGACCAAAGGAAATTTGCAATCTTTGTTGAAATTGTGGCATCGATAAATACTATTTGTTGGTTCAATCTTCTTTGAGCACACATT
GCATGAAATGTAGTACCAACCAAAATAGTTATGTATTTCTATTATCTTGCTCTTCACTGTAACAATGTATTCCTACAATTTTAATTAAATATCATGAAAT
AGTGTAATGATCATATTTAAATGGTAAACCAATTATGTGAGAAGCGTAATAAGAAAAGAACCTGTAGTTCAGAGCTCCACTCAGCCTCCAGCAACTCCTT
AATGTCCATACGGTTCAAAAACATCTCTTCCTCACGAGGAAGACTGTTAACATTAGAGCTTTTTATAATTTGAACTTCATTGGACATGGTGGAAAATTTT
GGAGCCAAAGATCTTATGTAATCTATATCAAGATTCACATATATTTTGCTTGCATAGGTGGTGGAGAAGCTAACCTCACCTAT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.951618
Expected error %
0.0501729
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65305.1:1-137 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.69%) Frame 2
Cloning vector pGEM-4Z
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65302.1:1-99 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.69%) Frame 2
Cloning vector pGEM-3
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0079P08
Clone
Clone name
hba-79p8
LE_HBa0079P08
P079P08
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found