Clone Read LE_HBa0174K10_T7_202856

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0174K10_T7_202856
Primer
T7
ID from Seq. Facility
LE_HBa_174_K10-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
683 trimmed for vector and quality, 805 raw
Sequence
>LE_HBa0174K10_T7_202856 (raw untrimmed)
ACTGGGACCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGCTGAACCAATCAAAGGCTTTGTTCCCTCAGTTGCGTTAGCGTGTACCACA
TCGATGTACAAAGTAGACTCTTCGATCGGAGCATACAACGGGATCACAAGCTTCTCATTCCAAGATGGTGAAGTATCGCCATTATCATCTACTTTGGTGG
AGCATTTCCCTTTCGGATCAACCCAAACAACAGCATATGGCTTAAGACGACCGTAACGCCAATTAACGTTCTTCAAATCCCTAGCTGATGAGATCTTCAC
CTCCACCTCGAATCGTGATCCCATATTTTTTTCTTCCCTCCCCCCTCAGAAGTGTAACAATGGCTGTTTTGTGATGGAGTGAGTATTATTTAACTGGTTT
TGGTTGTTTTAAGTTCCATTATGACCTTGGGCTTTTTCTATATGTTGGTGCTTGGTCTGTTTAAAACGGGAGAGTTGAGGAAGGGTATATTAGTCCAACA
AAATTTAAAAAAAAAAAAAAAGAGGAATTAGCGGATTAGGCCAGCCAAGCCGATAAGAGTTGAAAGAAACCGATCGAGGAGGTAGGACACGTGGACTCAT
ATGAGTGGTAGATAATGTGTTGTAATGAAAGACGTGTGGGGCCAGGACCTGGAGGAAGCAGAAAAGAGGAAAAACAAAAGCTCAATGTATTACCGTTTCG
ATTGTACTGTTTTATTTTAAATTTATTTAAAAATTAGTATGAGCATCCCCCTTCTAAAGATGTCAAGCAGCTTGCTTGCTTCCAAAATTAAAATTATGAG
TTTGT
>LE_HBa0174K10_T7_202856 (trimmed for quality and cloning vector)
AGCTTAGCTGAACCAATCAAAGGCTTTGTTCCCTCAGTTGCGTTAGCGTGTACCACATCGATGTACAAAGTAGACTCTTCGATCGGAGCATACAACGGGA
TCACAAGCTTCTCATTCCAAGATGGTGAAGTATCGCCATTATCATCTACTTTGGTGGAGCATTTCCCTTTCGGATCAACCCAAACAACAGCATATGGCTT
AAGACGACCGTAACGCCAATTAACGTTCTTCAAATCCCTAGCTGATGAGATCTTCACCTCCACCTCGAATCGTGATCCCATATTTTTTTCTTCCCTCCCC
CCTCAGAAGTGTAACAATGGCTGTTTTGTGATGGAGTGAGTATTATTTAACTGGTTTTGGTTGTTTTAAGTTCCATTATGACCTTGGGCTTTTTCTATAT
GTTGGTGCTTGGTCTGTTTAAAACGGGAGAGTTGAGGAAGGGTATATTAGTCCAACAAAATTTAAAAAAAAAAAAAAAGAGGAATTAGCGGATTAGGCCA
GCCAAGCCGATAAGAGTTGAAAGAAACCGATCGAGGAGGTAGGACACGTGGACTCATATGAGTGGTAGATAATGTGTTGTAATGAAAGACGTGTGGGGCC
AGGACCTGGAGGAAGCAGAAAAGAGGAAAAACAAAAGCTCAATGTATTACCGTTTCGATTGTACTGTTTTATTTTAAATTTAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.97438
Expected error %
0.383877
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g23950.1 Score 108 E Value 2e-24 Identity 20.70% Align. Length 260bp (38.07%) Frame 1
68418.m02812 C2 domain-containing protein similar to cold-regulated gene SRC2 [Glycine max] GI:2055230; contains Pfam profile PF00168: C2 domain
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g07310.1 Score 86 E Value 2e-17 Identity 14.40% Align. Length 263bp (38.51%) Frame 1
68414.m00778 C2 domain-containing protein contains similarity to shock protein SRC2 [Glycine max] gi|2055230|dbj|BAA19769 ; contains Pfam profile PF00168:C2 domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_483800.1| Score 127 E Value 3e-28 Identity 21.10% Align. Length 278bp (40.70%) Frame 1
putative C2 domain-containing protein [Oryza sativa (japonica cultivar-group)] ref|XP_507612.1| PREDICTED P0543D10.14 gene product [Oryza sativa (japonica cultivar-group)] ref|XP_507344.1| PREDICTED P0543D10.14 gene product [Oryza sativa (japonica cultivar-group)] dbj|BAD09616.1| putative C2 domain-containing protein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAI58613.1| Score 124 E Value 3e-27 Identity 20.40% Align. Length 278bp (40.70%) Frame 1
C2 domain-containing protein [Hordeum vulgare subsp. vulgare]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0174K10
Clone
Clone name
hba-174k10
LE_HBa0174K10
P174K10
Clone type
BAC
Contamination
none
Estimated length
111,491
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found