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Clone Read LE_HBa0174B02_SP6_202407

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0174B02_SP6_202407
Primer
SP6
ID from Seq. Facility
LE_HBa_174_B02-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
1 trimmed for vector and quality, 1555 raw
Sequence
>LE_HBa0174B02_SP6_202407 (raw untrimmed)
GGTCGTATATCTATCTAAGTGCGTGTGCTACAGTGGAATCTCATNCTCCCGGCTATTTTCTCTCTCCTCACTCACTCTTTACGCTATTTATAGCNTATAT
GATATGCGTTCGATNCTTNAGATTNCTGAAAGTGAGTCGAGACACCTTCCCCCTTATTGTATATCNGTCATTGCGTCCTGTTTTAAAAANTNCTATATGA
TGCACCGTGAGAGTGAAGACGTAGCGTAGCCCCGTACAGTGATAGGTGTAGAAGACGTGCGCTCTAGACTAGTTGCGCGAGACATGACGAGCTTGATGAA
CTAAGGTCGGCTGCGAGNTCGCTTGTGTCAAANTAATTTGTCGTATTATACTCTCGATATCAAGTGGTTAGACACGTATGAGATCGCACTGTTCGAANCT
TATACATCATCTACAGAGAGNGTATAGCTAATTCACATCTCAGTGTCATACTANACTACTCTGCGNCTTGCTTATGAATACATAGTCTATTATAGTCTCC
GCATACCACTCCGATACTGATAATTGTATATCGTACTGTGCTGAGCCGCTTACAGTGCGCATACTTGAACTGAAGTCACACGATGGTACCGNGTACTGNC
AGTAAGTTCATACTGCAACACACTCCCAGCTACGCACATGNTTTAATACAGTGACCTATTNCCTTGATGCTGTGCCTAGAAACTAGCTAACATGAGTGGT
ATAACGTTCATCTTATTATAAATCTATACGTGTGAGTGATNANCATACTAGATNGTCGCGCTAATATNTGGAGAATGATACTTACTATATTTGTACGCTC
TCGAGTGCTCTCTATAAAGATATGATGTTTCTAAGAGCTATCGATACATTAGCTCAATTGAACTCTATAGCACATAACTATCTCTATCGTCTATATCTAC
ATTATGTTTGGTACTACTTTTACACCATCCTCGACATTNTATTCAGATACGTCTTTTAGATGATATAGGAACGTTGTATTTGTTTTCATATCGTTCACTT
CGTATCACGTTGTATGCGTGTATTAATCTTCGCTACGATCTNCGGGTCTGTGACATTACATAATGTCTCCACGNCTGGACCTCGCACAACGCCTCATGAA
TTGCTCGTACCGCTCCGATACCACCAATTTTNATACATCTGCTTCGCGCACTCTCATATGTGTCTCATATCTCTCTCTGCTAGCCGTTGCTCCACTTCAT
AGTATCATCGTNACATACAGAAAAATCATCCCTCACTTTAATGTTCCCTACTATCTCATACTTATGCATATATAANGTCTCATTTCTGGAATGTAATATC
TGACATCTCCGCTAAACTTATACAGACAGTGCTACTTTACAGGTACTTATTACAGTTCATAAGTATATGCCTTATGTCTGATTGCNCNGAATTTNCTTGC
CTTCAGCTCTACTGCGTATGCTCGCACTACGTTACTCTTTATACTTTCGCAATCNCTCACTTCGCATTCTTGATGTACTACTTCACACTATCTTTTATAT
CCGCAATGTACTATCGTCACGCTACATATAGCGATCCTCTACCATTTCTGCATTA
>LE_HBa0174B02_SP6_202407 (trimmed for quality and cloning vector)
G

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
  • High predicted error rate
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
100
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0174B02
Clone
Clone name
hba-174b2
LE_HBa0174B02
P174B02
Clone type
BAC
Contamination
none
Estimated length
109,429
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found