Clone Read LE_HBa0155L16_SP6_200995

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0155L16_SP6_200995
Primer
SP6
ID from Seq. Facility
LE_HBa_155_L16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
712 trimmed for vector and quality, 916 raw
Sequence
>LE_HBa0155L16_SP6_200995 (raw untrimmed)
GGGAATTTAAAGTTGAATTCTTTACTTCCTTAATGACTCTGACATTGATGATAAATTTTTGAGCGTTGAAGAGTTCATATATTGCAGAAAGTCTATATAT
ATGGCTTTAGATGTTGAATAACGTTTGTGGAAAAATCCTATGCAAATTTGAATAGCATTCCAACTATGTGTTTGAATTTGACATGTGCGCTTGAGTCCAA
GTTGGTGCTGCTTTTGTTTGCATATTTGAGCAAAGGGATCTAGGACGCTATCATGATTAGGGGCCTTGATGATCTGGGTAGACATTTGTGTTAAGGGGTT
ATACATATGGTGAAGAGTGGATGCAGCCTCCCCTCCCCCATTGCCTTTCTGAGGGAACTTAAGCTCTAAGCCAAATTTTGTGCGATATTATATTCTTTTC
GAATTCAATTTTCTTTTCTTTGATCAGTATATGCAGCAACTAGCTAAGTTAATGACAATTTCTTGATTTCATTGTGGTGGTCCTCAAAAACAGGGTTTCA
ATTCTACACTGACAGAATCGGTGGATACCCCTGGAAGAAGAGGTGGAGATCAAATCAAAGAAATGGAACTGAAATTGCATCCTGAGTTGAAAGAATCATT
GCTCGCGATTTGTAACGACCCAAAGACAACAGTCGTTGTCCTCAGTGGAAGTGATAGAAACGTCTTAGATGATGTATTGCCTTACAACTAGCAATATTTC
AATTCCCTCAACTGTAGCGACGTTTCCCCATGTGAAATTTGTACTTTTTGCAGAACTTCAGCGAGTACAACATGTGGTTAGCAGCAGAAAATGGAATGTT
TTTACTATCTACAAACGGTGTTTGGATGACAACTATGCCAAACACCTAAACCTGGACTGGGTTAATAGTGTAAAGGTATGATCATGTTTACCTATTGTCT
GGATATGCACCTTCAC
>LE_HBa0155L16_SP6_200995 (trimmed for quality and cloning vector)
TCTTTACTTCCTTAATGACTCTGACATTGATGATAAATTTTTGAGCGTTGAAGAGTTCATATATTGCAGAAAGTCTATATATATGGCTTTAGATGTTGAA
TAACGTTTGTGGAAAAATCCTATGCAAATTTGAATAGCATTCCAACTATGTGTTTGAATTTGACATGTGCGCTTGAGTCCAAGTTGGTGCTGCTTTTGTT
TGCATATTTGAGCAAAGGGATCTAGGACGCTATCATGATTAGGGGCCTTGATGATCTGGGTAGACATTTGTGTTAAGGGGTTATACATATGGTGAAGAGT
GGATGCAGCCTCCCCTCCCCCATTGCCTTTCTGAGGGAACTTAAGCTCTAAGCCAAATTTTGTGCGATATTATATTCTTTTCGAATTCAATTTTCTTTTC
TTTGATCAGTATATGCAGCAACTAGCTAAGTTAATGACAATTTCTTGATTTCATTGTGGTGGTCCTCAAAAACAGGGTTTCAATTCTACACTGACAGAAT
CGGTGGATACCCCTGGAAGAAGAGGTGGAGATCAAATCAAAGAAATGGAACTGAAATTGCATCCTGAGTTGAAAGAATCATTGCTCGCGATTTGTAACGA
CCCAAAGACAACAGTCGTTGTCCTCAGTGGAAGTGATAGAAACGTCTTAGATGATGTATTGCCTTACAACTAGCAATATTTCAATTCCCTCAACTGTAGC
GACGTTTCCCCA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.965802
Expected error %
0.0548391
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g78580.1 Score 86 E Value 2e-17 Identity 24.30% Align. Length 176bp (24.72%) Frame 3
68414.m09158 alpha, alpha-trehalose-phosphate synthase, UDP-forming, putative / trehalose-6-phosphate synthase, putative / UDP-glucose-glucosephosphate glucosyltransferase, putative similar to trehalose-6-phosphate synthase SL-TPS/P [Selaginella lepidophylla] GI:4100325; contains Pfam profiles PF00982: Glycosyltransferase family 20, PF02358: Trehalose-phosphatase
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU44253.1| Score 96 E Value 1e-18 Identity 25.60% Align. Length 179bp (25.14%) Frame 3
putative trehalose-6-phosphate synthase (fragment) [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAK44102.2| Score 86 E Value 7e-16 Identity 24.30% Align. Length 176bp (24.72%) Frame 3
putative trehalose-6-phosphate synthase [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0155L16
Clone
Clone name
hba-155l16
LE_HBa0155L16
P155L16
Clone type
BAC
Contamination
none
Estimated length
44,043
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found