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Clone Read LE_HBa0076J16_SP6_17742

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0076J16_SP6_17742
Primer
SP6
ID from Seq. Facility
LE_HBa_076_J16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
520 trimmed for vector and quality, 841 raw
Genbank Accession
DU035436
Sequence
>LE_HBa0076J16_SP6_17742 (raw untrimmed)
CCGGGCTATTTGAGGATACCCAGCGAGAGGTTAGTGATTGTTACAATTGTTTCTGGAAACAAATGGATGTCAAGATTTTATGTTGTGGACTGAAATAATT
GTGGCGTTTTCGGAAATTGATCCTGAGAAAGCTATTCTCCTTTTTGGTCAGTTGCTCCGAGAGGGGTTAAGTTTAGATAGTTATGCTTTTTCTATTGCAC
TGAAAGCTTGTGCGGGACTCTTGACAGATAGAAATGCCTTGATAGTGCACTGTAAAGTGATTAAATCTGGTTTTGTGGATGCTCTAGTACTTGGAAATGC
ATTAATTCATGCATATGCAAGGTGTGGCTCAATATCTCGGGCCAGTCAGGTTTTTGAGGAGATGAGGTACAGAGATATAGTGACATGGAATTCCATGTTG
AAGGCTTATGCATTGCATGGCAAAGCAAATGAAGCATTGGGACTTTACAGTAAAATGGATGTGAAACCTGATGCAGCCACTTTTGTTGCTCTGCTTTCAG
CTTGCAGCCATGCTGGAATGGTGCAAGAAGGGATTCAAATTTTTGATGCTTTGTTTGCAAAACACTGTATTGTTTCCTCAACTCGAGCATTATGCATGTA
TAGTTGATTTTGTTGGCCGAGCAGGTCCTATTTTTTTTGTGTAGATATCAATAACAAAATGCTCCCGCAACCAGAATTTCTGGTGTGAGGAGGTTCATTC
CTTGGTTCATAGCGCCTAGATTCTTCGGTTCGGTGTTTGTTCTATAAATGTCCTTCTTCACTAAACCACGTTACCCCTTACTTTATCTCTATTAGATATC
AATAATCGGTAATAGATCAATATTCTTTCGCTCTTACTCTT
>LE_HBa0076J16_SP6_17742 (trimmed for quality and cloning vector)
AGGTTAGTGATTGTTACAATTGTTTCTGGAAACAAATGGATGTCAAGATTTTATGTTGTGGACTGAAATAATTGTGGCGTTTTCGGAAATTGATCCTGAG
AAAGCTATTCTCCTTTTTGGTCAGTTGCTCCGAGAGGGGTTAAGTTTAGATAGTTATGCTTTTTCTATTGCACTGAAAGCTTGTGCGGGACTCTTGACAG
ATAGAAATGCCTTGATAGTGCACTGTAAAGTGATTAAATCTGGTTTTGTGGATGCTCTAGTACTTGGAAATGCATTAATTCATGCATATGCAAGGTGTGG
CTCAATATCTCGGGCCAGTCAGGTTTTTGAGGAGATGAGGTACAGAGATATAGTGACATGGAATTCCATGTTGAAGGCTTATGCATTGCATGGCAAAGCA
AATGAAGCATTGGGACTTTACAGTAAAATGGATGTGAAACCTGATGCAGCCACTTTTGTTGCTCTGCTTTCAGCTTGCAGCCATGCTGGAATGGTGCAAG
AAGGGATTCAAATTTTTGAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.936304
Expected error %
0.348057
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g71420.1 Score 205 E Value 1e-53 Identity 19.40% Align. Length 500bp (96.15%) Frame 3
68414.m08249 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g36980.1 Score 129 E Value 9e-31 Identity 38.40% Align. Length 476bp (91.54%) Frame 3
68415.m04535 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_177298.1| Score 205 E Value 5e-52 Identity 19.40% Align. Length 500bp (96.15%) Frame 3
pentatricopeptide (PPR) repeat-containing protein [Arabidopsis thaliana] gb|AAG51830.1| hypothetical protein; 56014-58251 [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD31583.1| Score 129 E Value 4e-29 Identity 38.40% Align. Length 476bp (91.54%) Frame 3
hypothetical protein [Arabidopsis thaliana] pir||A84787 hypothetical protein At2g36980 [imported] - Arabidopsis thaliana ref|NP_181235.1| pentatricopeptide (PPR) repeat-containing protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0076J16
Clone
Clone name
hba-76j16
LE_HBa0076J16
P076J16
Clone type
BAC
Contamination
none
Estimated length
78,189
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found