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Clone Read LE_HBa0044H12_SP6_142811

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0044H12_SP6_142811
Primer
SP6
ID from Seq. Facility
LE_HBa_044_H12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
721 trimmed for vector and quality, 780 raw
Genbank Accession
DU043144
Sequence
>LE_HBa0044H12_SP6_142811 (raw untrimmed)
TTCTGGGGTACTCTGGATNGATTGTCAGCATTGCTTGATCGGGCGGAGGAATTGGGGCTAGATCTTCCTTATTCATGTAGGGCTGGTGCTTGCTCATCTT
GTGCTGGAAAGGTTACTGCTGGAAGTGTTGATCAGTCTGATAACAGTTTTCTTGATGATGACCAAATAGATGAAGGATTTGTGCTCACTTGTGTTGCTTA
TCCAAAGTCTAATGTTACCATTCAGACTCACAAGGAGGAGGACCTTGTTGGCTAACTAGTATTTTACTTCTTTTCTTATCAATAATTAAATTAGGTGCTA
TGTACCTCTTTTATATAAGTCAATGATGACTTGTACCATAATGATTTATGGAAGAATTAATAATTTTCTATTTTTATTTAACTCGTCTCATATTCACTTC
AAAAATCAGAATTTTCGTTAAGGAGTGTACGGTAATAAAAGATTAATTATAAGGATAATTGGACATGACCTCAAGAAATTTTCACTATTAGTGTCCCCTA
ATTCAGGAATTATAACAAACAAAGCAAAAAACAGATAACACATATGTTTTAATTGAAAACAATGCACGAAGAATAAATGGTACATTCTGTTTTGTTACAT
TACAGAATATCACCATTAATAAAATATCAAAGAGTAATACTTTATTCCCATAGCCTTGTGGCTATTGAAAATTAAATTTAAGTACAAAAGTCAACAAGTT
TTAGATGTGTCAGCACTACATTTTTTCCACTCCACTCTTCCCTCATGGTTTTATGTAGTAGTCGATATGTCAACATTGTG
>LE_HBa0044H12_SP6_142811 (trimmed for quality and cloning vector)
TGATCGGGCGGAGGAATTGGGGCTAGATCTTCCTTATTCATGTAGGGCTGGTGCTTGCTCATCTTGTGCTGGAAAGGTTACTGCTGGAAGTGTTGATCAG
TCTGATAACAGTTTTCTTGATGATGACCAAATAGATGAAGGATTTGTGCTCACTTGTGTTGCTTATCCAAAGTCTAATGTTACCATTCAGACTCACAAGG
AGGAGGACCTTGTTGGCTAACTAGTATTTTACTTCTTTTCTTATCAATAATTAAATTAGGTGCTATGTACCTCTTTTATATAAGTCAATGATGACTTGTA
CCATAATGATTTATGGAAGAATTAATAATTTTCTATTTTTATTTAACTCGTCTCATATTCACTTCAAAAATCAGAATTTTCGTTAAGGAGTGTACGGTAA
TAAAAGATTAATTATAAGGATAATTGGACATGACCTCAAGAAATTTTCACTATTAGTGTCCCCTAATTCAGGAATTATAACAAACAAAGCAAAAAACAGA
TAACACATATGTTTTAATTGAAAACAATGCACGAAGAATAAATGGTACATTCTGTTTTGTTACATTACAGAATATCACCATTAATAAAATATCAAAGAGT
AATACTTTATTCCCATAGCCTTGTGGCTATTGAAAATTAAATTTAAGTACAAAAGTCAACAAGTTTTAGATGTGTCAGCACTACATTTTTTCCACTCCAC
TCTTCCCTCATGGTTTTATGT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.946819
Expected error %
0.0555
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g60950.1 Score 124 E Value 4e-29 Identity 26.80% Align. Length 212bp (29.40%) Frame 3
68414.m06861 ferredoxin, chloroplast (PETF) identical to FERREDOXIN PRECURSOR GB:P16972 [SP|P16972] from [Arabidopsis thaliana]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g10960.1 Score 118 E Value 3e-27 Identity 24.90% Align. Length 212bp (29.40%) Frame 3
68414.m01258 ferredoxin, chloroplast, putative strong similarity to FERREDOXIN PRECURSOR GB:P16972 [SP|P16972] from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||S69935 Score 147 E Value 3e-34 Identity 32.40% Align. Length 215bp (29.82%) Frame 3
ferredoxin [2Fe-2S] II - tomato prf||2210387B ferredoxin:ISOTYPE=II
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
sp|P83583|FER_SOLLY Score 135 E Value 1e-30 Identity 29.20% Align. Length 215bp (29.82%) Frame 3
Ferredoxin
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0044H12
Clone
Clone name
hba-44h12
LE_HBa0044H12
C10HBa0044H12
P044H12
Clone type
BAC
Contamination
none
Estimated length
84,825
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found