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Clone Read LE_HBa0035A01_SP6_140917

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0035A01_SP6_140917
Primer
SP6
ID from Seq. Facility
LE_HBa_035_A01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
22 trimmed for vector and quality, 862 raw
Sequence
>LE_HBa0035A01_SP6_140917 (raw untrimmed)
CAAAAAATTCAAAGGCCCTTTGTGCGTCAAAGATACTTGGATCTGCAAATTATCCCCTTATAGGGGGACAAANCTTCCAAANNACCACNANGANCCCCTT
TTGGGGGGGACACANGGNCACAACAAAAGGAANNNGCAGGGCAAANGCANACGNANAAACCAGNACAACAATAANAGAAAAAACNGACCAACAAACCGAA
ACCCAACGCGGCAAACGGGAAACAAGNCGAAACAGGAACGAGGGAACAAAGCACACACAANGCNACAAAAACACACCAAAAACCCGAAAGACACGCGAAC
CGACGAACAAANACNGAGAACCCAAGACNCGCCCCACGACCNACCCCAAACCAANCNACAAACCGGGGACAACGCANCCGCACGACCNACANGACNNGAN
NCGCNCGGAANNGACANCAACCNCCGAGGCGGAACCCAAAGCAGAAACACGCGCNGAAAACCACCAACCCNCACAAAGGCCCNGCGGCAACAACACAGCC
ACCACCCCCCCCACAAACAGCACAACGAGCACCAAAACGCGCCACCAAAACANCAAAGAGGNGACAGCAAACACACGAAGAGGCGGGCGGAAACAGAAAA
CACAACNCAGGGAGAACACAAGGGAAGCGAACAAACCAAAAAAAAAAGCGCAAAGCCCCGGGCGACAAGAACCACGCCGCACACCGAAACCAAACCGGAA
CCCAGGAGGCGAAAGAAAAAAACACCAGGCCGGCCAGACGAAAAAGACACACCCCCGGCCGAGGGGGAGGGCCACCCAAACAACCGAGACCGGGGCCCGG
AACAGGAAGGGGAACCACCACGCAAACCAGCCACAAAAGGAGGACCCAGGGCAAGAAGGAAC
>LE_HBa0035A01_SP6_140917 (trimmed for quality and cloning vector)
ACAAAAGGAANNNGCAGGGCAA

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
  • High predicted error rate
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
18.8594
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0035A01
Clone
Clone name
hba-35a1
LE_HBa0035A01
C12HBa0035A01
P035A01
Clone type
BAC
Contamination
none
Estimated length
118,417
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found