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Clone Read LE_HBa0031A19_T7_139418

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0031A19_T7_139418
Primer
T7
ID from Seq. Facility
LE_HBa_031_A19-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
822 trimmed for vector and quality, 941 raw
Genbank Accession
DU048141
Sequence
>LE_HBa0031A19_T7_139418 (raw untrimmed)
TAAACTCGGTACCCGGGGGAACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGCATCATGAAGGTTCATGTGTGGGACTAAAAGAGGAATTGCCATCC
GGAGTCTAAGAGTCTCCTTGATCACAGCCTGAAGGTATGGAAGCTTGGGCATGTCTGGCTCACTCACTTGCACTCCTGGTCCAAGAACTGTATCAATCTC
GTCTCGGAGTTTCTTTTGGATGTGAGGGTGGTTGACTAGTTCCGCGATACCCCACTCAATTGACCACAATGTTGTTTCGATTGCTGTTCATGACAAGAAT
TAAGCATATCAGCTGAATAATCAACTAACCAAGAAGTTTATGAACAGATATAAACAACTAAACGTGCTTCAACGATTCTAAATGCTAGTTTTATTCGAGA
TCGTCCTCATCAAAGCTCAGAGGTAATTCCAAGATCTACAATTAGTGAGTTCAAAATGAGTATGATTTCTTCATATATATATAAGAGATCCTTCTTTGAT
ATGCATGCACTACAGACCAAAAGCAGTGAGTTCAGTAAAACTCAGAAACCTGCGTAAATCCATTTTTGTTGATCTTTTAGTTCATTACTCCACTATCATG
ATCATAATTAACATGAATTGTCGTCACAAGTCATTACTCAAGGAAAGTTAGATGCCTTGAAATGTGCACCAACAACCCTGCTCTACCTAACCCTCACCAA
GAAATTGGCAAGTACACCAACCATATCAAACTGTTACCTATCCACTTATTTGGAACTTCTCACCTACTACCTCTAATTAAATAACAGAATAAATCGGAAA
AGGGATCCATCAGGCTTTCGGCATTTCCGGAACTTCCCCACTGCTGCCCCTCAAAGGAATCTGGGCCATGTATCAGTCCTAGTGCAGTTAATCACCTTCT
AAACCAACTATTCATCATCCCCTGCAAAGTGATTGTCTCAT
>LE_HBa0031A19_T7_139418 (trimmed for quality and cloning vector)
AGCTTTGCATCATGAAGGTTCATGTGTGGGACTAAAAGAGGAATTGCCATCCGGAGTCTAAGAGTCTCCTTGATCACAGCCTGAAGGTATGGAAGCTTGG
GCATGTCTGGCTCACTCACTTGCACTCCTGGTCCAAGAACTGTATCAATCTCGTCTCGGAGTTTCTTTTGGATGTGAGGGTGGTTGACTAGTTCCGCGAT
ACCCCACTCAATTGACCACAATGTTGTTTCGATTGCTGTTCATGACAAGAATTAAGCATATCAGCTGAATAATCAACTAACCAAGAAGTTTATGAACAGA
TATAAACAACTAAACGTGCTTCAACGATTCTAAATGCTAGTTTTATTCGAGATCGTCCTCATCAAAGCTCAGAGGTAATTCCAAGATCTACAATTAGTGA
GTTCAAAATGAGTATGATTTCTTCATATATATATAAGAGATCCTTCTTTGATATGCATGCACTACAGACCAAAAGCAGTGAGTTCAGTAAAACTCAGAAA
CCTGCGTAAATCCATTTTTGTTGATCTTTTAGTTCATTACTCCACTATCATGATCATAATTAACATGAATTGTCGTCACAAGTCATTACTCAAGGAAAGT
TAGATGCCTTGAAATGTGCACCAACAACCCTGCTCTACCTAACCCTCACCAAGAAATTGGCAAGTACACCAACCATATCAAACTGTTACCTATCCACTTA
TTTGGAACTTCTCACCTACTACCTCTAATTAAATAACAGAATAAATCGGAAAAGGGATCCATCAGGCTTTCGGCATTTCCGGAACTTCCCCACTGCTGCC
CCTCAAAGGAATCTGGGCCATG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.967303
Expected error %
0.3139
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g30490.1 Score 147 E Value 5e-36 Identity 29.90% Align. Length 233bp (28.35%) Frame 1
68415.m03714 trans-cinnamate 4-monooxygenase / cinnamic acid 4-hydroxylase (C4H) (CA4H) / cytochrome P450 73 (CYP73) (CYP73A5) identical to SP|P92994| Trans-cinnamate 4-monooxygenase (EC 1.14.13.11) (Cinnamic acid 4-hydroxylase) (CA4H) (C4H) (P450C4H) (Cytochrome P450 73). {Arabidopsis thaliana}; molecular marker C4H (GB:U71080)
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g66540.1 Score 79 E Value 2e-15 Identity 15.80% Align. Length 227bp (27.62%) Frame 1
68414.m07560 cytochrome P450, putative Similar to cytochrome P450 91A1 (SP:Q9FG65)[Arabidopsis thaliana]; contains Pfam profile: PF00067: Cytochrome P450
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAB71717.1| Score 151 E Value 2e-35 Identity 31.60% Align. Length 233bp (28.35%) Frame 1
cinnamic acid 4-hydroxylase [Lithospermum erythrorhizon]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAB71716.1| Score 151 E Value 2e-35 Identity 31.20% Align. Length 233bp (28.35%) Frame 1
cinnamic acid 4-hydroxylase [Lithospermum erythrorhizon]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0031A19
Clone
Clone name
hba-31a19
LE_HBa0031A19
C02HBa0031A19
P031A19
Clone type
BAC
Contamination
none
Estimated length
95,966
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found