Clone Read LE_HBa0028M16_T7_138452

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0028M16_T7_138452
Primer
T7
ID from Seq. Facility
LE_HBa_028_M16-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
733 trimmed for vector and quality, 987 raw
Genbank Accession
DU047926
Sequence
>LE_HBa0028M16_T7_138452 (raw untrimmed)
CCCTTTGTGATGTTACTAAGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTAACTCTTTCATGGAAGCTGGCCTTTGCCAGCATACCATCCA
GAAGATATCAACCCTGCATCTTGGCTGCCGTCTCCATAGTGATTTTTTGAACTGTTGCTGGTTGTAGATATCTTCTTGTGCCAGTCCCTTCTGTCCATCG
TTTTGTGTGTTGCTTCATGATCTGCCCTGTCCAATCAAAACCTCATGTATTTTCTCATGTTTCTGTTCATTACAACTTGTGTGATTCTGTGTTGTGGACA
AGCAAAACGATGCCAGACAACTTATGATTTTGTGCGTGTGTGGTTTCAATCAGGTAAGCAGAAGACGGTAACCAGACAATTTTTTCTTGGGATATAGGAG
TTAATGCCCGTTACTTTTCGGACAACCTTGATCCATAAGAACTACCAAATCAAAATTTCTGAATCATGACAAGAAATATATTCTAGCAGAGCATGACTCT
GATGGTTGTTATCCCAGTATGAAGTTGATGCCACACTGTTATTCATGATTGGACTGGATCTTGCTAGGAAGCAAAGTTCTCTTCAATTATTCATGTAGTG
TTTATCAATCTGTTGAGGTGCAGCATACCAGGGTCTTCCCTTACAGTTGGAGACGGACAAGTTAATTGTTAACGGAGTGCTACTTTTCATGGTAGAATGT
TGGACCGTTCTTTTTCCCTATCCTTCTTAGTTATGTTCCATGAAACAATGCTGTGTGAACTGAAACTGTGAACTGAAACCAACTCCAGATTACTGTTCTC
TTCCCGTATTTTCCCTTTCAGAATTGTTTTCGGTCTGAAATCATTATTCAGTATAGTGGTCTATACCTGCCTCAACATTCCTTTATTTTTTTTTTTCTTT
AGGAAAAGAAAAAAATCCTGGAAAATTGTGAAACAATGGGAATATTTGAAGCCCTTTTTACCCCCTTTCCTTTTTTGGGAACACCCC
>LE_HBa0028M16_T7_138452 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTAACTCTTTCATGGAAGCTGGCCTTTGCCAGCATACCATCCAGAAGATATCAACCCTGCATCTTGGCT
GCCGTCTCCATAGTGATTTTTTGAACTGTTGCTGGTTGTAGATATCTTCTTGTGCCAGTCCCTTCTGTCCATCGTTTTGTGTGTTGCTTCATGATCTGCC
CTGTCCAATCAAAACCTCATGTATTTTCTCATGTTTCTGTTCATTACAACTTGTGTGATTCTGTGTTGTGGACAAGCAAAACGATGCCAGACAACTTATG
ATTTTGTGCGTGTGTGGTTTCAATCAGGTAAGCAGAAGACGGTAACCAGACAATTTTTTCTTGGGATATAGGAGTTAATGCCCGTTACTTTTCGGACAAC
CTTGATCCATAAGAACTACCAAATCAAAATTTCTGAATCATGACAAGAAATATATTCTAGCAGAGCATGACTCTGATGGTTGTTATCCCAGTATGAAGTT
GATGCCACACTGTTATTCATGATTGGACTGGATCTTGCTAGGAAGCAAAGTTCTCTTCAATTATTCATGTAGTGTTTATCAATCTGTTGAGGTGCAGCAT
ACCAGGGTCTTCCCTTACAGTTGGAGACGGACAAGTTAATTGTTAACGGAGTGCTACTTTTCATGGTAGAATGTTGGACCGTTCTTTTTCCCTATCCTTC
TTAGTTATGTTCCATGAAACAATGCTGTGTGAA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.972185
Expected error %
0.2247
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.23%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.23%) Frame 2
Cloning vector pT3/T7-LUC
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0028M16
Clone
Clone name
hba-28m16
LE_HBa0028M16
P028M16
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found