Clone Read LE_HBa0025J05_SP6_135981

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0025J05_SP6_135981
Primer
SP6
ID from Seq. Facility
LE_HBa_025_J05-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
501 trimmed for vector and quality, 924 raw
Genbank Accession
DU045123
Sequence
>LE_HBa0025J05_SP6_135981 (raw untrimmed)
CCCCGATCACCAACATAGAATCTCAAAGGTCGACGCCTCCGTTCTCATAACTCTGGTGCCTACTCTGAGCTGTCCTGAGTCTATCCTGAATCACTCTCAC
CTGCTCCAAAGCCTCCTGAATCAAATCTGTACCTCGCGGCCTAGGCTCTGTAGACTCGAACCAGCCCACTGGAGTGCGACAACGCCTACCATACAAGGCC
TCAAACGGGGCCATTTGAATGCTAGAATGGTAGCTGTTGTTATATGCAAATTCCGCCAAACGCAAGCACTAGTCCCATTGACCACCAAACTCCAAAACAC
AAGCCCGTAGCATGTCCTCAAGGACCTGAATAGTGCACTCTGACTGACCATCAGTCTGCGGGTGGAAAGTTGTGCTCAGGTCAACCCGGGTGCCCAACTC
ATCCTGAAAGGTCCTCCAAAAGCTAGATGTGAACTGAGAACCCCGATCTGATATAATAGAAATTGGCACCCCATGAAGTCGAACCACCTCCCGAATGTAG
ATACGAGCCATCCTCTCGGCACTAAATGAAGATTGAACAGGAAGGAAATGTGCTGACTTGGTCAATCCATCAACGATGACCCAATACTATCAACTCCTCT
AAAAGTCCTCGGTAGAACAGCCACAAAATCCATAGGAATGCGGGTCCTCCTTCCATTGCGGGAATGGGTAACCCCTGAAATTCACTCCAAGGCACTAAAT
GGTCGGCCTTTACCTGCTGGCGCAACAGCCACAGAAAGCAAGTCCGCTATATCTTGTCTCTGCCCCTCCCCCAGTAATGTGTCTCAATCACCAAATATTT
CTTGTCCCCCGATGGAAAAATATTGTACCTGGAGCCTGAAAGATTAACTGTTCATTTCCACTTCCGGGAAGAAGTGGGATGGAAGCTGACGACCATGGGA
AGAGGGACCGGTCCTCTTGGCGGA
>LE_HBa0025J05_SP6_135981 (trimmed for quality and cloning vector)
AATCTCAAAGGTCGACGCCTCCGTTCTCATAACTCTGGTGCCTACTCTGAGCTGTCCTGAGTCTATCCTGAATCACTCTCACCTGCTCCAAAGCCTCCTG
AATCAAATCTGTACCTCGCGGCCTAGGCTCTGTAGACTCGAACCAGCCCACTGGAGTGCGACAACGCCTACCATACAAGGCCTCAAACGGGGCCATTTGA
ATGCTAGAATGGTAGCTGTTGTTATATGCAAATTCCGCCAAACGCAAGCACTAGTCCCATTGACCACCAAACTCCAAAACACAAGCCCGTAGCATGTCCT
CAAGGACCTGAATAGTGCACTCTGACTGACCATCAGTCTGCGGGTGGAAAGTTGTGCTCAGGTCAACCCGGGTGCCCAACTCATCCTGAAAGGTCCTCCA
AAAGCTAGATGTGAACTGAGAACCCCGATCTGATATAATAGAAATTGGCACCCCATGAAGTCGAACCACCTCCCGAATGTAGATACGAGCCATCCTCTCG
G

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.971441
Expected error %
0.0921
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38745.1| Score 298 E Value 3e-80 Identity 30.60% Align. Length 470bp (93.81%) Frame 3
putative polyprotein, 3'-partial [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT66771.1| Score 292 E Value 2e-78 Identity 29.90% Align. Length 470bp (93.81%) Frame 3
putative polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0025J05
Clone
Clone name
hba-25j5
LE_HBa0025J05
P025J05
Clone type
BAC
Contamination
none
Estimated length
89,540
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found