Clone Read LE_HBa0024I03_T7_135162

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0024I03_T7_135162
Primer
T7
ID from Seq. Facility
LE_HBa_024_I03-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
762 trimmed for vector and quality, 954 raw
Genbank Accession
DU047096
Sequence
>LE_HBa0024I03_T7_135162 (raw untrimmed)
GCTCACTTGGACCGGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCATGTACTTGTTGATGAAGTCACTGCAAGTTATGAGAAATTTTTCTT
CGGAGATGTTGGAAGATAAATATATGACTTCTTTTGGTCTGATTTTGCTGATTGGTATGTTCTTCCTAGTGTTTTTGGTTCTTTTACTTGGTTTACGTGT
AAGTCTTGTTACTCACTTATTTAAAGAAGACATTATCCAGTATTATTTGAATAAGAAATTATGCAGTATGTGAAGTCCTAACCATTTAGAACCGGGAACA
AGTTTTGTTGAAGATTAATATGTAGAAGAATGATGCTGATGGTGGGAAGAAATTTAATAAGTAACTCTTCTTACACTAGCTGATCTAAAATTTGAAGTGT
ATGAGTTATTTCAGCAACCTCAGGTTAATACTCACTCTATCTTAATTTATGTGACACCATTTGGCCGGGCACGAAGTTTAAGAAATAAGGAAAGACTTTG
TTATGTATACCAAATTGCCCTTAATGTCACTTTAATGCTTACTTTAGTTGACTTTTCTTCACAATAATGTGTAAAGTTGGACCCAATAAGGATAAAATGG
GGATGATGTCATTATAGTTGCCAAATAAGGAAAAATGACACTCTTTTTGGGACAGACAGAAATGGAAAGGGTGCCACATAAATTGAGATAGAGGAAGTAT
ACATATGACTGGATCAACGTCAAATATTTATAGATATTTAATGAATTCCTTACTACATATTCAGGATTAAGAAAAAGCAATTGGATTTCTGTGACCCATA
ATAATCAATGTAGATCCACCTCCCCACCCATTATCTAAAATCTAAATTAGGACTCTTTTTATTGAAAATTATATGTAGAAAAATGATATTGTTGGTGGCA
TAGGAATTAATTTGGGATTCATTCTTACCCCCTTGGCAGGGGGATTGCCTTGTC
>LE_HBa0024I03_T7_135162 (trimmed for quality and cloning vector)
AGCTTCATGTACTTGTTGATGAAGTCACTGCAAGTTATGAGAAATTTTTCTTCGGAGATGTTGGAAGATAAATATATGACTTCTTTTGGTCTGATTTTGC
TGATTGGTATGTTCTTCCTAGTGTTTTTGGTTCTTTTACTTGGTTTACGTGTAAGTCTTGTTACTCACTTATTTAAAGAAGACATTATCCAGTATTATTT
GAATAAGAAATTATGCAGTATGTGAAGTCCTAACCATTTAGAACCGGGAACAAGTTTTGTTGAAGATTAATATGTAGAAGAATGATGCTGATGGTGGGAA
GAAATTTAATAAGTAACTCTTCTTACACTAGCTGATCTAAAATTTGAAGTGTATGAGTTATTTCAGCAACCTCAGGTTAATACTCACTCTATCTTAATTT
ATGTGACACCATTTGGCCGGGCACGAAGTTTAAGAAATAAGGAAAGACTTTGTTATGTATACCAAATTGCCCTTAATGTCACTTTAATGCTTACTTTAGT
TGACTTTTCTTCACAATAATGTGTAAAGTTGGACCCAATAAGGATAAAATGGGGATGATGTCATTATAGTTGCCAAATAAGGAAAAATGACACTCTTTTT
GGGACAGACAGAAATGGAAAGGGTGCCACATAAATTGAGATAGAGGAAGTATACATATGACTGGATCAACGTCAAATATTTATAGATATTTAATGAATTC
CTTACTACATATTCAGGATTAAGAAAAAGCAATTGGATTTCTGTGACCCATAATAATCAATG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.939402
Expected error %
0.6326
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g16715.1 Score 74 E Value 1e-13 Identity 27.00% Align. Length 110bp (14.44%) Frame 1
68418.m01957 tRNA synthetase class I (I, L, M and V) family protein similar to SP|P11931 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS) {Bacillus stearothermophilus}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_568337.3| Score 74 E Value 5e-12 Identity 27.00% Align. Length 110bp (14.44%) Frame 1
tRNA synthetase class I (I, L, M and V) family protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0024I03
Clone
Clone name
hba-24i3
LE_HBa0024I03
C12HBa0024I03
P024I03
Clone type
BAC
Contamination
none
Estimated length
43,294
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found