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Clone Read LE_HBa0024B09_T7_134838

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0024B09_T7_134838
Primer
T7
ID from Seq. Facility
LE_HBa_024_B09-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
659 trimmed for vector and quality, 983 raw
Genbank Accession
DU046805
Sequence
>LE_HBa0024B09_T7_134838 (raw untrimmed)
CGGAACCGGGGCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCATCAAGATAAGCTAGTTATATGTATGAGAAAAAAGCAATTAAGAGT
CAACGCAAGAAAATCAAACATCTAACATCTTTGAAGAAGAAAATTAGACAAAGAAATCTTTGATAATATAAAATAAACTTGAAAGTGTCAAAAGACACAT
TTTTTATCCTTAAAATATCACAGAATATCCTTGAAGTACTCTTATCAAGAGAAAAAGGCAAACTCAAGATTGAAAAACAAGAAGAATCAAAGAAATATTA
ACGTCCAACTAAAATTTATATAACAAACCTCATCACACCATTGTTGTCCAGAATGTGCCAATTTAAAAAACTAGAGTCTTTCATCTACCGTATTGATATC
CAATTCAATGCTGGATTGATAACATTAAGAGCATATATATAAGGTTTCTTTTCCCTATCAAAACAGAAAAAAATTAAAAAATACTTAAAACCGAGACAAC
AACAACTAAAAAAGAGACAAATACGAAACTAAAGAAGAGGTACAGAGCGTTCCACAAGTTCAATCAAGCTTGTATATATATTATGAATTAGGAAAATTAT
CCCAAAAACATCAAAATGAATTACCTCTTTTTTGCACCTGTATAAAGCCACTTAGTGACGTGTAACACATAAAGTTCCTCCAGATTCAAAATGTTGTGTG
AAGGGAGATGTTATTTACTTTCCAAGATTTTTCTTTCTTCCCCATTTTTTTGGTGACGCTTTCTTTTTGTTTTGATAAACTTGGGCTCTTTTTTTAGAAT
AAATTCTTTTATCCTATTGATGTGAGAAAAATCTTCATCAAGTAAAATAAAGTCAATCAAGAGTATGCAAGAATTTTTGGAAATCCTCTGGTGCCTGGGC
ATATAATAGAAAATTGGATTAATTCAAACCAAACCCTGGCAACTTTTTTTTGTTTATTTTTTTCTCACCTTACCTTCAATTTT
>LE_HBa0024B09_T7_134838 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTCATCAAGATAAGCTAGTTATATGTATGAGAAAAAAGCAATTAAGAGTCAACGCAAGAAAATCAAACATCT
AACATCTTTGAAGAAGAAAATTAGACAAAGAAATCTTTGATAATATAAAATAAACTTGAAAGTGTCAAAAGACACATTTTTTATCCTTAAAATATCACAG
AATATCCTTGAAGTACTCTTATCAAGAGAAAAAGGCAAACTCAAGATTGAAAAACAAGAAGAATCAAAGAAATATTAACGTCCAACTAAAATTTATATAA
CAAACCTCATCACACCATTGTTGTCCAGAATGTGCCAATTTAAAAAACTAGAGTCTTTCATCTACCGTATTGATATCCAATTCAATGCTGGATTGATAAC
ATTAAGAGCATATATATAAGGTTTCTTTTCCCTATCAAAACAGAAAAAAATTAAAAAATACTTAAAACCGAGACAACAACAACTAAAAAAGAGACAAATA
CGAAACTAAAGAAGAGGTACAGAGCGTTCCACAAGTTCAATCAAGCTTGTATATATATTATGAATTAGGAAAATTATCCCAAAAACATCAAAATGAATTA
CCTCTTTTTTGCACCTGTATAAAGCCACTTAGTGACGTGTAACACATAAAGTTCCTCCA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.909703
Expected error %
0.0548
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M16728.1:1-87 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.70%) Frame 2
S.aureus multiple cloning site vector upstream of beta-lactamase gene, clone pWN1818
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65333.1:1-122 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.70%) Frame 2
Cloning vector pSP73
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0024B09
Clone
Clone name
hba-24b9
LE_HBa0024B09
P024B09
Clone type
BAC
Contamination
none
Estimated length
125,415
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found