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Clone Read LE_HBa0020D06_T7_132624

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0020D06_T7_132624
Primer
T7
ID from Seq. Facility
LE_HBa_020_D06-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
679 trimmed for vector and quality, 996 raw
Genbank Accession
DU051591
Sequence
>LE_HBa0020D06_T7_132624 (raw untrimmed)
GAAAGACCGGGTCCGGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGACTCCATATAAAAACATAAGTCAACTTGACATTCATTATATCCA
AATAATCAATTTATAACATGATAAAGCCATAATACTTAAAGTAATGTGGACGAGGCCGTATAATTCATAATTCATAAAGTAGTGCCGACAAGGCCACATT
TATCAATAAATAGCACATAATCACCGCCACCATAAGTGGACCATAACAAGATTCATAATCATCTAAGAACTAGAACAAACAATACAGAGAAAGTTAGGTT
AATATACGATGAATCAAACCCCACAAATTCAATCCATAGCCAATTCATCCAACACAATACTAAAGACCCTTACCCATGGCCATGATCATCAATTAAACCC
ATTAATTACAATACATAATGAATAAAATATAATTCAACATTGATTTATGGAGTTAGGGAAGCGTACTAACAATTTTAGCGTGATTCTATCATAATTCAAT
AGCCCTAATAAATCTACACAATAATTCAATAGCATTTAACCCATAAATTAGTCAAAACTGGAAGTTCATGGTTTGCATGGGTTCATAGATTTTTTTGGTT
AAAATCATCAAATTAACATCAATTCAATCATAATATAAAGTTTCAAAACTTTTAATTCAAGAACCCATGGATAGATTACAAAATTACACAATTCATGTTT
TTGATCATCTTTTGAAATCATTGGCATTGACTCCTTTATGAAAAAGATTTCAAGGATGAAAATCTATACCCTAATTGTGACGAAAATCAAAAAGATTGAT
GATGAAAACTTGAGAAAATTAGTCTTATCCTTGGACTTTCAACCTTTCTCATGGAAGGTTTTGGAAAAAAAAAGAAGAGGTTTTGATTTGATTTAAGGTT
TTGGATTTTTTGGGTGAGGTTAGAATTTAAAAAGGAACATAGCCCTCCTTAATTCCCCCCAAATCACCCAATTCCCCCGAACTTTTTGGAAAATTA
>LE_HBa0020D06_T7_132624 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGACTCCATATAAAAACATAAGTCAACTTGACATTCATTATATCCAAATAATCAATTTATAACATGATA
AAGCCATAATACTTAAAGTAATGTGGACGAGGCCGTATAATTCATAATTCATAAAGTAGTGCCGACAAGGCCACATTTATCAATAAATAGCACATAATCA
CCGCCACCATAAGTGGACCATAACAAGATTCATAATCATCTAAGAACTAGAACAAACAATACAGAGAAAGTTAGGTTAATATACGATGAATCAAACCCCA
CAAATTCAATCCATAGCCAATTCATCCAACACAATACTAAAGACCCTTACCCATGGCCATGATCATCAATTAAACCCATTAATTACAATACATAATGAAT
AAAATATAATTCAACATTGATTTATGGAGTTAGGGAAGCGTACTAACAATTTTAGCGTGATTCTATCATAATTCAATAGCCCTAATAAATCTACACAATA
ATTCAATAGCATTTAACCCATAAATTAGTCAAAACTGGAAGTTCATGGTTTGCATGGGTTCATAGATTTTTTTGGTTAAAATCATCAAATTAACATCAAT
TCAATCATAATATAAAGTTTCAAAACTTTTAATTCAAGAACCCATGGATAGATTACAAAATTACACAATTCATGTTTTT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.918707
Expected error %
0.179
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.57%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.57%) Frame 2
Cloning vector pT3/T7-LUC
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0020D06
Clone
Clone name
hba-20d6
LE_HBa0020D06
C10HBa0020D06
P020D06
Clone type
BAC
Contamination
none
Estimated length
139,477
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found