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Clone Read LE_HBa0019A16_SP6_131731

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0019A16_SP6_131731
Primer
SP6
ID from Seq. Facility
LE_HBa_019_A16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
587 trimmed for vector and quality, 869 raw
Genbank Accession
DU048828
Sequence
>LE_HBa0019A16_SP6_131731 (raw untrimmed)
AAGGGGGCCTTTCTGTNGCATTTAAATNGACACTCTGGGTATTGTGGTTACAATTGCCATCCGGAAAAAGACCTTTGCCATGTAAATGGGTTTACAAGGT
CAAGCAAAACTCAGATGGGTCTATTGAAAGACTGAAAGCTAGATTGGTGGTTAGGGGGGACATTCAGAAAGAAGGTGTTGACTTTAGTGAGACTTTTTCT
CCAGTGGTGAAGATCACCACTATCAAATGCTTGCTCACTATTGCCGCAAAGAAGGGATGGCAAGTGTCTCAACTTGATGTAAATAATGCGTTTCTGCATG
GAGAACTACAAGAGGAAGTGTACATGAGATTTCCTACTGGGTTTTCTCCACCTTCTCCAAACCATGTTTGTCTTTTGAAGAAGTCCCTCTATGGATTAAA
GCAGGCTTCACGACAGTGGTATGCCCGACTTGCTGGTGCTTTGGCATATAAAGGGTACTCTAGTTCTCTTAATGACTATTCTTTATTCTTTAAGAAAAAT
GGTGATCTTATATCAATCTTAGCTGTGTATGTAGATGACATACTACTAACTGGGAGTGACACCTCTGAGATTGCTTCTATCACTACTTTCCTCAACTCTG
AGTTTCGGGTTAAGAATTTGGGATACATTCACTATTTCTTAGGTATCGAAGTGATTAGAGAGAAACAGGGATTCATTTTATGTCAGCGTAAATTAACTTC
GGGATCTTTTAGGCCAAGTTTTGATTCTTCTGGATCTGTGGTATCTTCCTCCCCTAGACCCCTTATTTCCAAACTACAACATGATGAATGGGCCCTTTTT
TGGAATTTTTCCTTCTATTTTCCAGAAACTTTTGATAGGAAAAATTTAATTTTTCTCTTCTCCATCACT
>LE_HBa0019A16_SP6_131731 (trimmed for quality and cloning vector)
CAATTGCCATCCGGAAAAAGACCTTTGCCATGTAAATGGGTTTACAAGGTCAAGCAAAACTCAGATGGGTCTATTGAAAGACTGAAAGCTAGATTGGTGG
TTAGGGGGGACATTCAGAAAGAAGGTGTTGACTTTAGTGAGACTTTTTCTCCAGTGGTGAAGATCACCACTATCAAATGCTTGCTCACTATTGCCGCAAA
GAAGGGATGGCAAGTGTCTCAACTTGATGTAAATAATGCGTTTCTGCATGGAGAACTACAAGAGGAAGTGTACATGAGATTTCCTACTGGGTTTTCTCCA
CCTTCTCCAAACCATGTTTGTCTTTTGAAGAAGTCCCTCTATGGATTAAAGCAGGCTTCACGACAGTGGTATGCCCGACTTGCTGGTGCTTTGGCATATA
AAGGGTACTCTAGTTCTCTTAATGACTATTCTTTATTCTTTAAGAAAAATGGTGATCTTATATCAATCTTAGCTGTGTATGTAGATGACATACTACTAAC
TGGGAGTGACACCTCTGAGATTGCTTCTATCACTACTTTCCTCAACTCTGAGTTTCGGGTTAAGAATTTGGGATACATTCACTATTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.971114
Expected error %
0.039
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 192 E Value 9e-50 Identity 16.00% Align. Length 581bp (98.98%) Frame 2
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89730.1| Score 362 E Value 3e-99 Identity 30.40% Align. Length 584bp (99.49%) Frame 2
putative polyprotein [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAR24647.1| Score 220 E Value 2e-56 Identity 18.20% Align. Length 581bp (98.98%) Frame 2
At2g23330 [Arabidopsis thaliana] gb|AAM98191.1| unknown protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0019A16
Clone
Clone name
hba-19a16
LE_HBa0019A16
P019A16
Clone type
BAC
Contamination
none
Estimated length
84,412
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found