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Clone Read LE_HBa0018G15_SP6_131249

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0018G15_SP6_131249
Primer
SP6
ID from Seq. Facility
LE_HBa_018_G15-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
661 trimmed for vector and quality, 890 raw
Sequence
>LE_HBa0018G15_SP6_131249 (raw untrimmed)
TTCCCTTTTGGCGGTTANGATTTTATGTTCAACAACAGCCTTTTAATATGAACCAGACAACTCTAAGACTAGAGAAGACATCACAGACAACCAAATAAGA
GAAAGAAACTGATTCAACCACCAAACCCAGAGGTACAACCTATCCGGATAAATTTGTAAAAGGAAACCATTATCCCAAAGCTGACCCAGTCTGAAGCAAC
TTCAGAAGAGGGAAATCACTTGGCCATCATGACCTTAACAAACTCATCGTAGTTAATTTGTCCATCGCCATCGACATCAGCCTCCCTGATCATTTCATCA
ACTTCTTCATCAGTAAGCTTCTCACCAAGGTTAGTCATCACATGACGGAGCTCAGCAGCAGAGATGAACCCATTCTGGTCCTTGTCAAAACACTCTGAAT
GCCTCCTTCAGCTCCTCCTCTGAGTCTGTATCCTTCATCTTCCTGGCCATGAGATTCAAAAACTCTGGGAAGTCGATGGTTCCATTACCATCAGCATCCA
CTTCAGTTATCATGTCCTGGTGCTCGGCGCAGCTCAGTTGGGTTCTGTCCCAACGACCTCATCACAGTCCCAAGCTCCTTAGTTGTAATGCAACCTATTC
AAACCAACAATATGAGCAAACTAAACCAGAAGATCAAACAACGTATGCTTCTGACTGCATCTTTTACCAGTGCATACTTTCAAAAGAAACCAAATGTAAA
GTTAACCATACCAGATAATATCACCTAGTGTTTCAATTTGAACATGAGAACTCGTGATTCTCCACCCACGTCATTGACCACTAGGCCCAAGGATTTTGCT
TTTTTAAAAGAAAAAAAACACAAGTACCCTTTTAAATGCTTGGGGAACTAATTACAGAAATCTGCCAGGAAATTCTCTCCTATCCCGGGC
>LE_HBa0018G15_SP6_131249 (trimmed for quality and cloning vector)
CCTTTTAATATGAACCAGACAACTCTAAGACTAGAGAAGACATCACAGACAACCAAATAAGAGAAAGAAACTGATTCAACCACCAAACCCAGAGGTACAA
CCTATCCGGATAAATTTGTAAAAGGAAACCATTATCCCAAAGCTGACCCAGTCTGAAGCAACTTCAGAAGAGGGAAATCACTTGGCCATCATGACCTTAA
CAAACTCATCGTAGTTAATTTGTCCATCGCCATCGACATCAGCCTCCCTGATCATTTCATCAACTTCTTCATCAGTAAGCTTCTCACCAAGGTTAGTCAT
CACATGACGGAGCTCAGCAGCAGAGATGAACCCATTCTGGTCCTTGTCAAAACACTCTGAATGCCTCCTTCAGCTCCTCCTCTGAGTCTGTATCCTTCAT
CTTCCTGGCCATGAGATTCAAAAACTCTGGGAAGTCGATGGTTCCATTACCATCAGCATCCACTTCAGTTATCATGTCCTGGTGCTCGGCGCAGCTCAGT
TGGGTTCTGTCCCAACGACCTCATCACAGTCCCAAGCTCCTTAGTTGTAATGCAACCTATTCAAACCAACAATATGAGCAAACTAAACCAGAAGATCAAA
CAACGTATGCTTCTGACTGCATCTTTTACCAGTGCATACTTTCAAAAGAAACCAAATGTAA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.944269
Expected error %
0.0897
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g43810.1 Score 115 E Value 3e-55 Identity 98.20% Align. Length 376bp (56.88%) Frame 2
68416.m04682 calmodulin-7 (CAM7) almost identical to calmodulin GI:16227 from [Arabidopsis thaliana], SP|P59220 Calmodulin-7 {Arabidopsis thaliana}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g27030.2 Score 114 E Value 3e-48 Identity 96.50% Align. Length 343bp (51.89%) Frame 2
68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical to calmodulin GI:474183 from [Arabidopsis thaliana], SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA54583.1| Score 117 E Value 4e-54 Identity 100.00% Align. Length 376bp (56.88%) Frame 2
calmodulin [Zea mays] pir||S51933 calmodulin cam2 - maize
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAS13433.1| Score 117 E Value 4e-54 Identity 100.00% Align. Length 376bp (56.88%) Frame 2
calmodulin [Nicotiana attenuata] emb|CAD20351.1| calmodulin 2 [Brassica oleracea] gb|AAT40502.1| calmodulin NtCaM9 [Solanum demissum] gb|AAF65511.1| calmodulin [Capsicum annuum] sp|P93087|CALM_CAPAN Calmodulin (CaM) gb|AAB46588.1| calmodulin [Capsicum annuum] dbj|BAB61916.1| calmodulin NtCaM10 [Nicotiana tabacum] dbj|BAB61915.1| calmodulin NtCaM9 [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0018G15
Clone
Clone name
hba-18g15
LE_HBa0018G15
C02HBa0018G15
P018G15
Clone type
BAC
Contamination
none
Estimated length
98,006
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found